Morphological and molecular evidence for a new species of Lutzia (Diptera: Culicidae: Culicini) from Thailand

Phanitchakun, Thanari; Namgay, Rinzin; Miyagi, Ichiro; Tsuda, Yuichi; Walton, Catherine; Harbach, Ralph E.; Somboon, Pradya

doi:10.1016/j.actatropica.2018.12.029

Cited by 14 publications

(20 citation statements)

References 7 publications

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“…Because their COI nucleotide sequences were low interspecific differences (the distance to NN = 1.6%). Similarly, a previous study in Thailand reported that nucleotide sequences of Lt. fuscana and Lt. chiangmaiensis COI exhibited low interspecific variations (genetic distances = 0.2-2.4%) [68]. However, ML phylogenetic analysis can assist in the assessment of both species based on this study.…”

Section: Plos Onementioning

confidence: 75%

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Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae) in Thailand

et al. 2022

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The correct identification of mosquito species is important for effective mosquito vector control. However, the standard morphological identification of mosquito species based on the available keys is not easy with specimens in the field due to missing or damaged morphological features during mosquito collections, often leading to the misidentification of morphologically indistinguishable. To resolve this problem, we collected mosquito species across Thailand to gather genetic information, and evaluated the DNA barcoding efficacy for mosquito species identification in Thailand. A total of 310 mosquito samples, representing 73 mosquito species, were amplified using mitochondrial cytochrome c oxidase subunit I (COI) primers. The average maximum intraspecific genetic variation of the 73 mosquito species was 1% ranged from 0–5.7%. While, average minimum interspecific genetic variation (the distance to the nearest neighbour) of the 73 mosquito species was 7% ranged from 0.3–12.9%. The identification of success rates based on the “Best Match,” “Best Close Match,” and “All Species Barcodes” methods were 97.7%, 91.6%, and 81%, respectively. Phylogenetic analyses of Anopheles COI sequences demonstrated a clear separation between almost all species (except for those between An. baimaii and An. dirus), with high bootstrap support values (97%–99%). Furthermore, phylogenetic analyses revealed potential sibling species of An. annularis, An. tessellatus, and An. subpictus in Thailand. Our results indicated that DNA barcoding is an effective molecular approach for the accurate identification of mosquitoes in Thailand.

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Section: Plos Onementioning

confidence: 75%

“…fuscana and Lt . chiangmaiensis COI exhibited low interspecific variations (genetic distances = 0.2–2.4%) [ 68 ]. However, ML phylogenetic analysis can assist in the assessment of both species based on this study.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae) in Thailand

et al. 2022

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“…Distribution: Bhutan, China, India, Indonesia, Japan, Malaysia, Mariana Islands, Myanmar, Nepal, South-Korea, Sri Lanka, Taiwan and Thailand [ 56 , 66 ]. Collected in Kampong Cham and Battambang provinces.…”

Section: Subfamily Culicinae Meigen 1818mentioning

confidence: 99%

Checklist of the mosquito fauna (Diptera, Culicidae) of Cambodia

et al. 2021

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Between 2016 and 2020, the Medical and Veterinary Entomology unit of the Institut Pasteur du Cambodge collected over 230,000 mosquitoes. Based on this sampling effort, a checklist of 290 mosquito species in Cambodia is presented. This is the first attempt to list the Culicidae fauna of the country. We report 49 species for the first time in Cambodia. The 290 species belong to 20 genera: Aedeomyia (1 sp.), Aedes (55 spp.), Anopheles (53 spp.), Armigeres (26 spp.), Coquillettidia (3 spp.), Culex (57 spp.), Culiseta (1 sp.), Ficalbia (1 sp.), Heizmannia (10 spp.), Hodgesia (3 spp.), Lutzia (3 spp.), Malaya (2 spp.), Mansonia (5 spp.), Mimomyia (7 spp.), Orthopodomyia (3 spp.), Topomyia (4 spp.), Toxorhynchites (4 spp.), Tripteroides (6 spp.), Uranotaenia (27 spp.), and Verrallina (19 spp.). The Cambodian Culicidae fauna is discussed in its Southeast Asian context. Forty-three species are reported to be of medical importance, and are involved in the transmission of pathogens.

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“…Some species of the subgenus Metalutzia have limited distribution: Lt. agranensis is reported only in India; Lt. chiangmaiensis is reported only in northern Thailand. Other species of this subgenus are widely distributed: Lt. fuscana and Lt. halifaxii are distributed in the Oriental, Australian, and eastern Palearctic regions; Lt. tigripes is distributed throughout the Afrotropical region; and Lt. vorax is distributed in the Oriental and Australian regions [ 1 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Modern Techniques for Species Identification of Lutzia Mosquitoes (Diptera: Culicidae) in Thailand: Geometric Morphometrics and DNA Barcoding

Laojun

Changbunjong

Chaiphongpachara

2023

Insects

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There are four species of Lutzia mosquitoes in Thailand, including Lutzia chiangmaiensis, Lt. fuscana, Lt. halifaxii, and Lt. vorax. The accurate species identification of adult Lutzia mosquitoes based on morphological features requires many body parts, including the abdominal terga and wing. However, species identification is difficult in the case of damaged specimens when some of their morphological character is missing due to transit or gathering in the field. Thus, we evaluated the efficacy of the landmark-based geometric morphometric (GM) approach for the discrimination of Lutzia species in Thailand. In addition, DNA barcoding was also used in parallel with the GM approach to identify the species. Larvae of Lutzia were collected, raised into adults, and identified based on their morphological characteristics. The validated reclassification test results clearly demonstrated that wing shape resulted in a high level of success in identification (correct identifications ranged from 92.50% to 100%); however, based on the DNA barcoding analyses, our results showed that it was poorly effective in identifying Lt. fuscana and Lt. halifaxii based on an overlap between the intraspecific and interspecific divergence. Moreover, our survey results provide updates on the distribution of Lt. chiangmaiensis and Lt. vorax in Thailand. This research will help medical entomologists more efficiently identify mosquitoes in the genus Lutzia, resulting in more effective mosquito control and surveillance.

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Morphological and molecular evidence for a new species of Lutzia (Diptera: Culicidae: Culicini) from Thailand

Cited by 14 publications

References 7 publications

Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae) in Thailand

Mitochondrial DNA barcoding of mosquito species (Diptera: Culicidae) in Thailand

Checklist of the mosquito fauna (Diptera, Culicidae) of Cambodia

Evaluation of Modern Techniques for Species Identification of Lutzia Mosquitoes (Diptera: Culicidae) in Thailand: Geometric Morphometrics and DNA Barcoding

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