Morphological and molecular characterization of Paragonimus westermani in northeastern India

Devi, K. Rekha; Narain, Kanwar; Agatsuma, Takeshi; Blair, David; Nagataki, Mitsuru; Wickramasinghe, Susiji; Yatawara, Lalani; Mahanta, Jagadish

doi:10.1016/j.actatropica.2010.05.003

Cited by 30 publications

(32 citation statements)

References 16 publications

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“…It is also difficult to determine metacercariae and eggs of P. westermani to species level, since they are very similar to that of other digeneans morphologically. Furthermore, freshwater crabs or crayfish with only a few metacercariae harbored cannot be reliably recognized as infected using microscopic examination (Devi et al 2010). Detection of eggs in early or late infection stage or extrapulmonary infection stage is difficult, because eggs rarely exist in these stages.…”

Section: Introductionmentioning

confidence: 99%

Sensitive and rapid detection of Paragonimus westermani infection in humans and animals by loop-mediated isothermal amplification (LAMP)

Chen

Zhang

et al. 2010

Parasitol Res

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In the present study, a loop-mediated isothermal amplification (LAMP) assay was developed and validated for the detection of Paragonimus westermani adults, metacercariae, and eggs in human and animal samples. The LAMP amplification can be finished in 45 min under isothermal condition at 60°C by employing a set of four species-specific primer mixtures and the results can be checked by naked-eye visualization. No amplification products were detected with deoxyribunucleic acid (DNA) of related trematode species including Fasciola hepatica, Fasciola gigantica, Clonorchis sinensis, Opisthorchis viverrini, Schistosoma mansoni, and Schistosoma japonicum. The method was further validated by examining P. westermani DNA in intermediate hosts including freshwater crabs and crayfish, as well as in sputum and pleural fluid samples from patients of paragonimiasis. These results indicated that the LAMP assay was highly specific, sensitive, and rapid, and it was approximately 100 times more sensitive than conventional specific PCR. The LAMP assay established in this study provides a rapid and sensitive tool for the detection of P. westermani DNA in freshwater crabs, crayfish, sputum, and pleural fluid samples, which has important implications for effective control of human paragonimiasis.

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Section: Introductionmentioning

confidence: 99%

Sensitive and rapid detection of Paragonimus westermani infection in humans and animals by loop-mediated isothermal amplification (LAMP)

Chen

Zhang

et al. 2010

Parasitol Res

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“…As in the present study, natural infections of P. westermani metacercariae have been reported in B. masoniana in several foci of Arunachal Pradesh (North-east India) (Tandon et al 2007;Devi et al 2010). Potamiscus manipurensis, Alcomon superciliosum and Barytelphusa lugubris are identified as common potential second intermediate hosts for other Paragonimus species as well including P. heterotremus, P. hueit'ungensis, and P. skrjabini in Northeast India (Singh and Singh 1997;Singh 2002Singh , 2003Singh et al 2006Singh et al , 2007Singh et al , 2009Singh et al , 2012.…”

Section: Discussionmentioning

confidence: 69%

“…In context of India, the focal transmission of paragonimiasis has been documented in north-eastern states of the country, such as Arunachal Pradesh, Manipur and Nagaland (WHO 2009). Several species of Paragonimus have been reported to occur in the commonly edible crab species prevailing in the mountainous ranges of Northeast India (Singh 2002(Singh , 2003Narain et al 2003;Singh et al 2006Singh et al , 2007Singh et al , 2009Tandon et al 2007;Devi et al 2010). The regions, where crabs are commonly consumed as part of their traditional cuisine are suspected foci for human infection (Tandon et al 2007).…”

Section: Introductionmentioning

confidence: 99%

A survey of metacercarial infections in commonly edible fish and crab hosts prevailing in Manipur, Northeast India

Athokpam

Tandon

2013

J Parasit Dis

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Food-borne trematode infections, which are mainly transmitted through consumption of inadequately cooked or raw fish and crabs, affect a large section of population, particularly in Southeast Asian countries, thus eliciting a remarkable morbidity and causing serious damage to health. In India, centering in several mountainous regions of the Northeast, the natives have the habit of consuming such fish or crabs that still sustain viable infective larval stage (metacercaria) of trematode flukes in their muscle tissue. The present study was undertaken to ascertain the spectrum of metacercarial diversity in commonly edible freshwater fishes and crab species in the northeastern state of Manipur and to adjudge their zoonotic potential, if any. Commonly edible fishes belonging to 15 species from 12 localities and crabs belonging to 2 species from 11 localities across Manipur state were surveyed for the purpose. The study revealed that 3 species of fishes (Channa punctatus, C. straitus and Wallago attu) harboured 4 different types of metacercariae belonging to 4 trematode families-Euclinostomum heterostomum (Clinostomidae); Lophosicyadiplostomum sp. and Posthodiplostomum sp. (Diplostomidae); and Polylekithum sp. (Allocreadiidae) in addition to adult flukes of Isoparorchis hypselobagri (Isoparorchiidae). Among these, metacercariae of Posthodiplostomum showed the highest prevalence (2.33 %) though a low abundance, while for other species the prevalence ranged between 0.25 and 1.19 %. The crab species (Barythelphusa lugubris masoniana and Potamiscus manipuriensis) were found infected with 4 different types of metacercariae representing the genera Paragonimus (Troglotrematidae) and Microphallus (Microphallidae). The paragonimids showed a higher rate of occurrence (*4-25 %) compared to microphallids (*15 %). The crustaceans surveyed emerged as prospective intermediate hosts for lungflukes. Identifying the potent vectors for zoonotic parasites helps in control measures towards their transmission to higher mammals.

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“…Populations differ in their prepatent period [ 79 ]. Populations also differ in their preferred mammal hosts and in their snail hosts.…”

Section: Taxonomy Of Paragonimus Speciesmentioning

confidence: 99%

“…This is most apparent in the type species of the genus, Paragonimus westermani (Kerbert, 1878) Braun, 1899, described from the lungs of a Bengal tiger that had died in Amsterdam Zoo in 1877 [ 77 ]. It turns out that " P. westermani " is very widespread, occurring in eastern and southern Asia, from China ( and adjacent parts of SE Russia [ 78 ]), Japan and Korea west to India [ 79 ] and south to Sri Lanka [ 80 ], the Philippines [ 81 ], and possibly Papua New Guinea [ 82 ]. (Note that reports of endemic paragonimiasis caused by Paragonimus westermani in other parts of the world are likely to be erroneous, often based on the assumption that human cases are always due to this species.…”

Section: Taxonomy Of Paragonimus Speciesmentioning

confidence: 99%

Paragonimiasis

Blair

2014

Advances in Experimental Medicine and Biology

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Morphological and molecular characterization of Paragonimus westermani in northeastern India

Cited by 30 publications

References 16 publications

Sensitive and rapid detection of Paragonimus westermani infection in humans and animals by loop-mediated isothermal amplification (LAMP)

Sensitive and rapid detection of Paragonimus westermani infection in humans and animals by loop-mediated isothermal amplification (LAMP)

A survey of metacercarial infections in commonly edible fish and crab hosts prevailing in Manipur, Northeast India

Paragonimiasis

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