Morphological and molecular characteristics of Plasmodium juxtanucleare in layer chicken from three districts of Yogyakarta, Indonesia

Abstract

Background and Aim: Blood parasite infections in poultry, such as Plasmodium, are a serious threat to the poultry industry due to their potential to cause economic losses. To date, there has been inadequate research on the morphological and molecular detection of the different Plasmodium species that infect poultry in Indonesia. Therefore, this study aimed to analyze the morphological and molecular characteristics of Plasmodium spp. and the several predisposing factors for Plasmodium infection in layer chickens from three districts of Yogyakarta, Indonesia. Materials and Methods: One hundred and five blood samples from layer chickens were collected from 13 farms located in three districts of Yogyakarta (Sleman, Bantul, and Kulon Progo) between September and November 2022. Blood samples were subjected to microscopic and polymerase chain reaction (PCR) analyses. Sequencing was performed using basic local alignment search tools to identify the nucleotide structure of cytochrome b. Phylogenetic analysis of Plasmodium was performed using the MEGA-X software. Results: Microscopic examination revealed that 17/105 positives (16.19%) were positive for blood parasite infection. Trophozoites, erythrocytic meronts, and microgametocytes of Plasmodium were found in blood samples. Based on the morphological examination, the species found in the samples was close to Plasmodium juxtanucleare. Polymerase chain reaction examination revealed that 21/60 samples were positive for Plasmodium (35%). The Plasmodium species identified from the sequenced samples were proven to be P. juxtanucleare. The P. juxtanucleare from Thailand was closely related to samples (99.64%–100%) with a genetic distance of 0%–1%. In addition, age, population, and cage type were not significantly associated with Plasmodium infection. Conclusion: Based on microscopic and PCR examinations, the Plasmodium species found in the three districts of Yogyakarta was P. juxtanucleare. The genetic distance between samples from the three districts of Yogyakarta was closely related (0%–1%) to P. juxtanucleare from Thailand and Japan. There was no correlation between Plasmodium infection and age, cage type, or population. Keywords: avian malaria, cytochrome b gene, layer chicken, polymerase chain reaction.

Molecular prevalence, phylogeny and hematological impact of Toxoplasma gondii and Plasmodium spp. in common quails from Punjab, Pakistan

“…The presence of this parasite was detected among all enrolled bird species. Several Plasmodium species have been reported from birds, including poultry and farmed birds such as Plasmodium durae, Plasmodium gallinaceum, P. relictum, and P. juxtanucleare [13]. During the present study, DNA sequencing followed by BLAST analysis confirmed the presence of P. relictum in local quails.…”

“…Additionally, over 250 species of haemosporidians parasites, including Plasmodium, Haemoproteus, Leucocytozoon, and Fallisia, have been reported in birds [11]. Avian malarial parasites, specifically avian Plasmodium, are capable of developing and completing their life cycles in various bird species and their invertebrate vectors [12,13]. Mosquitoes from the Culicidae family, including genera such as Culex, Coquillettidia, Aedes, Mansonia, Culisetta, Anopheles, and Psorophora, are involved in the transmission of these parasites [14].…”