Morphological and Mechanical Characterization of Extracellular Vesicles and Parent Human Synoviocytes under Physiological and Inflammatory Conditions

Filali, Samira; Darragi-Raies, Nesrine; Ben-Trad, Layth; Piednoir, Agnès; Hong, Saw-See; Pirot, Fabrice; Landoulsi, Ahmed; Girard-Egrot, Agnès; Granjon, Thierry; Maniti, Ofélia; Miossec, Pierre; Trunfio-Sfarghiu, Ana-Maria

doi:10.3390/ijms232113201

Cited by 3 publications

(1 citation statement)

References 71 publications

(102 reference statements)

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“…WBC counts obtained from HT-stented mice were notably higher than those from EP-stented animals, even at 8 weeks postimplantation, indicating a persistent source of peri-implant inflammation. At the micro (cellular) level, it is well-known that several pathological conditions are closely related to alterations in cells’ structure, morphology, and nanomechanical properties. − The mechanical properties of live cells can affect their physical interactions with the surrounding extracellular matrix, potentially influencing the process of mechanical signal transduction in living tissues. Previous studies have described the infiltration of inflammatory cells and immunocytes (T-lymphocytes) in the restenotic tissue.…”

Section: Discussionmentioning

confidence: 99%

Multilevel Assessment of Stent-Induced Inflammation in the Adjacent Vascular Tissue

Kapnisis

Stylianou

Kokkinidou

et al. 2023

ACS Biomater. Sci. Eng.

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A recent U.S. Food and Drug Administration report presented the currently available scientific information related to biological response to metal implants. In this work, a multilevel approach was employed to assess the implant-induced and biocorrosion-related inflammation in the adjacent vascular tissue using a mouse stent implantation model. The implications of biocorrosion on peri-implant tissue were assessed at the macroscopic level via in vivo imaging and histomorphology. Elevated matrix metalloproteinase activity, colocalized with the site of implantation, and histological staining indicated that stent surface condition and implantation time affect the inflammatory response and subsequent formation and extent of neointima. Hematological measurements also demonstrated that accumulated metal particle contamination in blood samples from corroded-stetted mice causes a stronger immune response. At the cellular level, the stent-induced alterations in the nanostructure, cytoskeleton, and mechanical properties of circulating lymphocytes were investigated. It was found that cells from corroded-stented samples exhibited higher stiffness, in terms of Young’s modulus values, compared to noncorroded and sham-stented samples. Nanomechanical modifications were also accompanied by cellular remodeling, through alterations in cell morphology and stress (F-actin) fiber characteristics. Our analysis indicates that surface wear and elevated metal particle contamination, prompted by corroded stents, may contribute to the inflammatory response and the multifactorial process of in-stent restenosis. The results also suggest that circulating lymphocytes could be a novel nanomechanical biomarker for peri-implant tissue inflammation and possibly the early stage of in-stent restenosis. Large-scale studies are warranted to further investigate these findings.

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Section: Discussionmentioning

confidence: 99%

Multilevel Assessment of Stent-Induced Inflammation in the Adjacent Vascular Tissue

Kapnisis

Stylianou

Kokkinidou

et al. 2023

ACS Biomater. Sci. Eng.

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Effects of pro-inflammatory cytokines and cell interactions on cell area and cytoskeleton of rheumatoid arthritis synoviocytes and immune cells

Filali

Noack

Géloën

et al. 2023

European Journal of Cell Biology

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Causal influences of osteoarthritis on COVID-19: a Mendelian randomization study

Fu,

Baranova,

Cao

et al. 2023

Front. Med.

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ObjectiveAlthough observational and genetic studies have indicated a correlation between OA and COVID-19, it remains uncertain whether osteoarthritis (OA) contributes to the severity of COVID-19. Here, we aimed to investigate the potential causal links between the two.MethodsIn this study, we conducted Mendelian randomization (MR) analysis to investigate whether there is a potential causal connection between OA and COVID-19 outcomes. The analysis utilized publicly available GWAS summary datasets, incorporating data on OA (N = 455,221), SARS-CoV-2 infection (N = 2,597,856), hospitalized COVID-19 (N = 2,095,324), and critical COVID-19 (N = 1,086,211). Additionally, we performed a literature analysis to establish a molecular network connecting OA and COVID-19.ResultsThe MR analysis showed causal effects of OA on hospitalized COVID-19 (OR: 1.21, 95% CI: 1.02–1.43, p = 0.026) and critical COVID-19 (OR: 1.35, 95% CI: 1.09–1.68, p = 0.006) but not on SARS-CoV-2 infection as such (OR: 1.00, 95% CI: 0.92–1.08, p = 0.969). Moreover, the literature-based pathway analysis uncovered a set of specific genes, such as CALCA, ACE, SIRT1, TNF, IL6, CCL2, and others, that were found to mediate the association between OA and COVID-19.ConclusionOur findings indicate that OA elevates the risk of severe COVID-19. Therefore, larger efforts should be made in the prevention of COVID-19 in OA patients.

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Morphological and Mechanical Characterization of Extracellular Vesicles and Parent Human Synoviocytes under Physiological and Inflammatory Conditions

Cited by 3 publications

References 71 publications

Multilevel Assessment of Stent-Induced Inflammation in the Adjacent Vascular Tissue

Multilevel Assessment of Stent-Induced Inflammation in the Adjacent Vascular Tissue

Effects of pro-inflammatory cytokines and cell interactions on cell area and cytoskeleton of rheumatoid arthritis synoviocytes and immune cells

Causal influences of osteoarthritis on COVID-19: a Mendelian randomization study

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