Morphological and histological effects of copper sulfate on the larval development of green toad, Bufo viridis

Gürkan, Mert; Hayretdağ, Sibel

doi:10.3906/zoo-0909-18

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…Histological abnormalities caused by copper sulphate included tissue deformation observed through a cross-section of the liver as a result of degeneration of hepatocytes and increase intercellular areas. These same deformations were observed in the epithelia of the pronephric tubules and somites, in addition to poor development [15].…”

Section: Histological Changessupporting

confidence: 68%

“…Copper sulphate is the chemical compound with the chemical formula : CuSO4, 5H2O; it is a protectant foliar fungicide often used in ponds to control algae [56]. This chemical had a 120h LC50 of 0.058 mg/L (Table 2) with the larvae of the green toad Bufo viridis [15]. Note:…”

Section: Fungicidesmentioning

confidence: 99%

“…Abnormal behavioural responses were also observed with Atrazine in tadpoles and the response was positively correlated with the concentration gradient [71]. Some of these adverse behavioural effects included reduced reaction to stimuli, short swimming distance and loss of equilibrium induced by copper sulphate in tadpoles of the greed toad, Bufo viridis [15]. Atrazine reduced amphibian water-conserving behaviours, which increased their rate of water loss.…”

Section: Behavioural Changesmentioning

confidence: 99%

“…Deformation was observed in the somites of the tadpoles that were exposed to 0.05 and 0.1 mg/L of Copper Sulphate [15]. Histological abnormalities caused by copper sulphate included tissue deformation observed through a cross-section of the liver as a result of degeneration of hepatocytes and increase intercellular areas.…”

Section: Histological Changesmentioning

confidence: 99%

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Effects of Pesticides on Amphibians and Tentative Solutions: Review

Kenko

2022

5003

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Amphibians are among the main affected non-target groups, victim of pesticides. This review puts into evidence worldwide studies on lethal and sub-lethal effects of pollutants on amphibians and proposes solutions to handle the issue. Literature review was carried out from articles and books, on 123 studies. The number of pesticide formulations used worldwide in very high. Pesticide effect on the environment and the biota in general have been assessed in many studies using tools such as biomonitoring, bioassays in laboratory and semi-field conditions and Ecological Risk Assessment (EcoRA) with models. The ecological effects of pollutants are varied and often inter-related. Effects in the organism or at the ecological level are usually considered an early warning indicator of potential human health impact. Effects can either be lethal or sub-lethal comprising cancer, tumours, lesions, reduction of reproductive potential, immune suppression, disruption of endocrine system, cellular and Deoxyribonucleic acid (DNA) damage, and teratogenic effects. Amphibian conservation can be done via the protection of genetic resources, cleaning habitat, sustainable harvesting, captive breeding, cloning and reintroduction programmes. Proper use of pesticides and reinforcement of regulation remains a necessity to protect environmental health.

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Section: Histological Changessupporting

confidence: 68%

Section: Fungicidesmentioning

confidence: 99%

Section: Behavioural Changesmentioning

confidence: 99%

Section: Histological Changesmentioning

confidence: 99%

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Effects of Pesticides on Amphibians and Tentative Solutions: Review

Kenko

2022

5003

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“…The results of the present study show clearly that the tissue of the livers of the P. cuvieri tadpoles from the five sites analyzed had suffered major alterations, as well as an inflammatory response, as confirmed by the presence of melanomacrophages, steatosis, and the infiltration of inflammatory cells. These histopathological alterations, which may represent the irreversible degeneration of the liver cells, may have occurred in response to the pesticides applied to plantations adjacent to the habitats occupied by the tadpoles (Gürkan & Hayretdag ˘, 2012).…”

Section: Histopathology and Ultrastructure Of The Livermentioning

confidence: 99%

The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach

Lopes

Benvindo‐Souza

Sotero

et al. 2023

Enviro Toxic and Chemistry

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Changes in the natural landscape and the indiscriminate use of pesticides can have a major impact on aquatic environments and have contributed to the worldwide decline of amphibian populations. In the present study, we sampled tadpoles of three anuran amphibians (Boana albopunctata, Physalaemus cuvieri, and Dendropsophus minutus) from ponds in six different agricultural landscapes of the Brazilian Cerrado savanna and evaluated whether and to what extent genotoxic and mutagenic damage was related to land use (the amount of forest and agricultural remnants, and related physicochemical factors) and the presence of pesticides in the water of the study ponds. We also evaluated the hepatotoxicity in P. cuvieri, which was the most abundant species at five of the six sampling points. Clomazone and Atrazine were the most common pesticides found in the ponds. The B. albopunctata and P. cuvieri tadpoles presented similar patterns of DNA damage among the sampling points. The least DNA damage was found in the D. minutus tadpoles, although this species was present in only one of the study ponds. More binucleated and anucleated cells were observed in B. albopunctata, but there was no significant variation among species in terms of the number of micronuclei or other erythrocytic nuclear abnormalities. Land use and physicochemical factors did not explain the variation in the DNA damage observed in the three anurans. The hepatotoxicity analyses of P. cuvieri revealed the presence of a series of alterations, including the enlargement of the sinusoids, vacuolization of the hepatocytes, the infiltration of inflammatory cells, hepatic steatosis, and dilation of the blood vessels. The interaction between physicochemical factors and the biomarkers analyzed in this study is complex. In particular, it will be important to better elucidate which factors are contributing, either directly or indirectly, to the decline of anuran amphibian populations, especially in threatened biomes, such the the Brazilian Cerrado. In this case, we would encourage further in situ studies that assess the ecotoxicology of the landscape, together with the systematic monitoring of aquatic environments, in order to guarantee the long‐term integrity of amphibian populations, and those of other organisms that play an essential functional role in the ecosystem.

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