Morphological and Genetic Investigation of Mediaeval Grape Seeds

Bodor, P.; Deák, Tamás; Bacsó, Renáta; Velich, I.; Bisztray, Gy.; Facsar, G.; Gyulai, Ferenc

doi:10.17660/actahortic.2006.725.100

Cited by 4 publications

(2 citation statements)

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“…It also does not require template DNA to be ultra pure, which is a requirement in AFLP analysis since contaminated or impure DNA is often recalcitrant in restriction enzyme digestions to produce nonspecific spurious bands. This is a real benefit when one is dealing with specimens that are dry, contaminated, mummified or even in fossilized form in the wild [ 87 , 88 ].…”

Section: Advantages Of Ssr Analysismentioning

confidence: 99%

Simple Sequence Repeat Polymorphisms (SSRPs) for Evaluation of Molecular Diversity and Germplasm Classification of Minor Crops

2009

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Evaluation of the genetic diversity among populations is an essential prerequisite for the preservation of endangered species. Thousands of new accessions are introduced into germplasm institutes each year, thereby necessitating assessment of their molecular diversity before elimination of the redundant genotypes. Of the protocols that facilitate the assessment of molecular diversity, SSRPs (simple sequence repeat polymorphisms) or microsatellite variation is the preferred system since it detects a large number of DNA polymorphisms with relatively simple technical complexity. The paucity of information on DNA sequences has limited their widespread utilization in the assessment of genetic diversity of minor or neglected crop species. However, recent advancements in DNA sequencing and PCR technologies in conjunction with sophisticated computer software have facilitated the development of SSRP markers in minor crops. This review examines the development and molecular nature of SSR markers, and their utilization in many aspects of plant genetics and ecology.

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Section: Advantages Of Ssr Analysismentioning

confidence: 99%

Simple Sequence Repeat Polymorphisms (SSRPs) for Evaluation of Molecular Diversity and Germplasm Classification of Minor Crops

2009

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“…In this regard, molecular marker techniques are advantageous over the morphological measurements for the assessment of genetic diversity as they directly reflect variations in the DNA sequence which are free from environmental influences and provide complementary information. Among many molecular marker techniques currently available, microsatellites or Simple sequence repeats (SSRs) are very useful for DNA fingerprinting, assessing genetic diversity and genetic relationships in plants as they are highly polymorphic [6], co-dominant [8,9], able for multiplex PCR [10], reproducible [11,12], well distribution in genome [13,14] and can be integrated with high throughput instrumentation [15] making them valuable tool for genetics and breeding. Advancement in sequencing technologies, directly made sequencing increasingly available to embark into genomic level.…”

Section: Introduction mentioning

confidence: 99%

Development of Starfruit Simple Sequence Repeat (SSR) Using Next Generation Sequencing

Nasir¹,

Yusof²,

Firdaus³

et al. 2019

JFSE

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Starfruit (Averrhoa carambola L.) is an important fruit for Malaysian export and great attention has been made to improve starfruit fruit quality at Malaysian Agricultural Research and Development Institute (MARDI). The current study used next generation sequencing (NGS) technologies to develop starfruit simple sequence repeat (SSR) from 2 varieties namely B11 and B17 using Illumina HiSeq. The pre-processed reads were de novo assembled to generate approximately 75,000 and 74,000 scaffolds respectively. Total genome size for B11 and B17 were around 345 Mbp and 342 Mbp based on K-mer distribution analysis. In-silico microsatellite mining of each variety has identified more than 17,000 SSR in B11 and B17 respectively. Dinucleotides were the most abundant, accounting for more than 70% of all SSR and repeat motif GA (49%) was most common. A total of 239 SSR primer pairs were designed from contigs larger than 350 nucleotides and tested for amplification. The 30 polymorphic SSRs were used to DNA fingerprint of 12 starfruit hybrids. Polymorphism information content (PIC) ranged from 0.1411 to 0.6838, with an average of 0.3919. The Unweighted Pair-Group Method for Arithmetic Averages (UPGMA) dendrogram clustered 12 starfruit accessions into 2 groups.

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QTL analysis for phytonutrient compounds and the antioxidant molecule in mustard (Brassica juncea L.)

et al. 2014

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Morphological and Genetic Investigation of Mediaeval Grape Seeds

Cited by 4 publications

References 0 publications

Simple Sequence Repeat Polymorphisms (SSRPs) for Evaluation of Molecular Diversity and Germplasm Classification of Minor Crops

Simple Sequence Repeat Polymorphisms (SSRPs) for Evaluation of Molecular Diversity and Germplasm Classification of Minor Crops

Development of Starfruit Simple Sequence Repeat (SSR) Using Next Generation Sequencing

QTL analysis for phytonutrient compounds and the antioxidant molecule in mustard (Brassica juncea L.)

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