Morphological, Anatomical, and Phytochemical Studies of Carlina acaulis L. Cypsela

Strzemski, Maciej; Płachno, Bartosz J.; Mazurek, B.; Kozłowska, Weronika; Sowa, Ireneusz; Lustofin, Krzysztof; Załuski, Daniel; Rydzik, Łukasz; Szczepanek, Dariusz; Sawicki, Jan; Wójciak–Kosior, Magdalena

doi:10.3390/ijms21239230

Cited by 6 publications

(9 citation statements)

References 50 publications

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“…The oil is a semi-solid, yellow-green substance with a characteristic aroma ( Figure 8 A) absorbing UV–VIS radiation in the range of 190–430 nm and at a wavelength of 660–680 nm ( Figure 8 B). Absorbance at a wavelength of 269 nm may indicate the presence of tocopherols, especially α-tocopherol absorbing radiation in the range of 265–310 nm [ 24 ]. Absorbance at 407 and 660–680 nm may indicate the presence of chlorophylls [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

Pharmacognostic Evaluation and HPLC–PDA and HS–SPME/GC–MS Metabolomic Profiling of Eleutherococcus senticosus Fruits

et al. 2021

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Eleutherococcus senticosus (Rupr. et Maxim.) Maxim. is a medicinal plant used in Traditional Chinese Medicine (TCM) for thousands of years. However, due to the overexploitation, this species is considered to be endangered and is included in the Red List, e.g., in the Republic of Korea. Therefore, a new source of this important plant in Europe is needed. The aim of this study was to develop pharmacognostic and phytochemical parameters of the fruits. The content of polyphenols (eleutherosides B, E, E1) and phenolic acids in the different parts of the fruits, as well as tocopherols, fatty acids in the oil, and volatile constituents were studied by the means of chromatographic techniques [HPLC with Photodiode-Array Detection (PDA), headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry (HS–SPME/GC–MS)]. To the best of our knowledge, no information is available on the content of eleutherosides and phenolic acids in the pericarp and seeds. The highest sum of eleutheroside B and E was detected in the whole fruits (1.4 mg/g), next in the pericarp (1.23 mg/g) and the seeds (0.85 mg/g). Amongst chlorogenic acid derivatives (3-CQA, 4-CQA, 5-CQA), 3-CQA was predominant in the whole fruits (1.08 mg/g), next in the pericarp (0.66 mg/g), and the seeds (0.076 mg/g). The oil was rich in linoleic acid (C18:3 (n-3), 18.24%), ursolic acid (35.72 mg/g), and α-tocopherol (8.36 mg/g). The presence of druses and yellow oil droplets in the inner zone of the mesocarp and chromoplasts in the outer zone can be used as anatomical markers. These studies provide a phytochemical proof for accumulation of polyphenols mainly in the pericarp, and these structures may be taken into consideration as their source subjected to extraction to obtain polyphenol-rich extracts.

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Section: Resultsmentioning

confidence: 99%

Pharmacognostic Evaluation and HPLC–PDA and HS–SPME/GC–MS Metabolomic Profiling of Eleutherococcus senticosus Fruits

et al. 2021

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“…The content of chlorogenic acid was measured using an RP18e LiChrospher 100 column (Merck, Darmstadt, Germany) (25 cm × 4.0 mm i.d., 5 µm particle size) according to the procedure published previously [ 62 ].…”

Section: Methodsmentioning

confidence: 99%

High Temperature Alters Secondary Metabolites and Photosynthetic Efficiency in Heracleum sosnowskyi

Rysiak

Dresler

Hanaka

et al. 2021

IJMS

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Due to global warming, invasive species have spread across the world. We therefore studied the impact of short-term (1 day or 2 days) and longer (7 days) heat stress on photosynthesis and secondary metabolites in Heracleum sosnowskyi, one of the important invasive species in the European Union. H. sosnowskyi leaves exposed to short-term heat stress (35 °C/1 d) showed a decrease in chlorophyll and maximum potential quantum efficiency of photosystem II (Fv/Fm) compared to control, 35 °C/2 d, or 30 °C/7 d treatments. In turn, the high level of lipid peroxidation and increased H2O2 accumulation indicated that the 30 °C/7 d stress induced oxidative damage. The contents of xanthotoxin and bergapten were elevated in the 2 d and 7 d treatments, while isopimpinellin was detected only in the heat-stressed plants. Additionally, the levels of free proline and anthocyanins significantly increased in response to high temperature, with a substantially higher increase in the 7 d (30 °C) treatment. The results indicate that the accumulation of proline, anthocyanins, and furanocoumarins, but not of phenolic acids or flavonols, contributes to protection of H. sosnowskyi plants against heat stress. Further studies could focus on the suppression of these metabolites to suppress the spread of this invasive species.

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“…Many scientific reports have confirmed its multidirectional biological activity, including antioxidant, antibacterial, antifungal, insecticidal, and anti-ulcer properties and cytotoxicity against a few types of cancer lines [ 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. The plant has also been shown to be a rich source of valuable components including volatile compounds of essential oil, phenolic acids, and pentacyclic triterpenes [ 22 , 23 , 24 , 25 ]. These findings justify the traditional usage of Carlina species and demonstrate the enormous potential of the genus.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Profiling, Antioxidant Activity, and Protective Effect against H2O2-Induced Oxidative Stress of Carlina vulgaris Extract

et al. 2023

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Carlina vulgaris is a little-understood plant with unexplored biological potential, and the papers regarding its chemical composition are scarce. In our study, for the first time, the phytochemical profile of the plant, focusing on polar metabolites, was established using modern chromatographic techniques including LC-HRMS-QTOF-CAD, UHPLC-PDA-MS. Phytochemical analysis revealed that the species is a rich source of polyphenolic components, with the most abundant being chlorogenic acid and C-glycosides of luteolin, including carlinoside, orientin, isoorientin, and C-glycosides of apigenin, schaftoside, isoschaftoside, and vitexin. Furthermore, we assessed the impact of the polyphenolic-rich fraction of C. vulgaris extracts on human skin fibroblasts using the MTT and NR assays. It was found that the extract was non-toxic and exhibited potent antioxidant activity in the cells subjected to induced oxidative stress. Additionally, it effectively protected the cells against H2O2-induced cytotoxicity. Our study contributes to the general trend of searching for new phytotherapeutics with potential applications in pharmacy and medicine. The results indicate that further exploration of C. vulgaris species is worthwhile, as they can serve as valuable plant material for cosmetic use.

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Morphological, Anatomical, and Phytochemical Studies of Carlina acaulis L. Cypsela

Cited by 6 publications

References 50 publications

Pharmacognostic Evaluation and HPLC–PDA and HS–SPME/GC–MS Metabolomic Profiling of Eleutherococcus senticosus Fruits

Pharmacognostic Evaluation and HPLC–PDA and HS–SPME/GC–MS Metabolomic Profiling of Eleutherococcus senticosus Fruits

High Temperature Alters Secondary Metabolites and Photosynthetic Efficiency in Heracleum sosnowskyi

Phytochemical Profiling, Antioxidant Activity, and Protective Effect against H2O2-Induced Oxidative Stress of Carlina vulgaris Extract

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