Morphological analysis and DNA methylation in Conyza bonariensis L. cronquist (Asteraceae) phenotypes

Paula, Juliana Maria de; Pinto-Maglio, Cecília Alzira Ferreira; Pinto, Luciana Rossini

doi:10.1590/1678-4499.2016.379

Cited by 5 publications

(5 citation statements)

References 31 publications

(27 reference statements)

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“…These modifications play a controlled role in regulating the expression of neighboring genes [13,14]. Consequently, without altering the DNA sequence, they have a profound influence on leaf development and morphogenesis [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Heritable Epigenetic Modification of BpIAA9 Causes the Reversion Mutation of Leaf Shapes in Betula pendula ‘Dalecarlica’

Zhang,

Gu,

Jiang

et al. 2024

Forests

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The European white birch, scientifically known as Betula pendula, and its variant, B. pendula ‘Dalecarlica’, are characterized by a lobed leaf margin that enhances their aesthetic appeal. However, the lobed leaf trait can undergo random and spontaneous reversion to the typical ovoid or cordate shape during asexual reproduction. Investigating and elucidating the molecular mechanisms underlying this unpredictable reversion mutation is essential for comprehending the birch leaf development process. In this research, we employed an ovate−leaf mutant derived from a lobed–leaf birch clone during plant tissue culture. We adopted a multi-omics approach, including whole-genome resequencing, transcriptome sequencing, and methylation profiling, to analyze and compare genomic variations and gene expression modifications. The study revealed that the 24 variant genes affected by 1464 SNP/InDel sites in the genome of the ovate−leaf mutant are not associated with leaf development. While the overall methylation level in the mutant’s genome closely resembles that of the lobed–leaf birch, ten genes exhibit differential methylation accompanied by differential expression. Transcriptome sequencing demonstrated that the differentially downregulated genes in the mutant are significantly enriched in the GO:0009733 (response to auxin) and GO:0009734 (auxin-activated signaling) pathways. Validation through McrBC−PCR and qRT−PCR confirmed differential methylation and expression of BpIAA9 in the reversion mutant. The elevated methylation level in the BpIAA9 promoter leads to reduced expression, resulting in changes in the expression of auxin-responsive genes. This, in turn, leads to a transcriptional downregulation enrichment effect in auxin-related pathways in the reversion mutant, ultimately inhibiting the regulation of leaf veins by auxin during their development.

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Section: Introductionmentioning

confidence: 99%

Heritable Epigenetic Modification of BpIAA9 Causes the Reversion Mutation of Leaf Shapes in Betula pendula ‘Dalecarlica’

Zhang,

Gu,

Jiang

et al. 2024

Forests

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“…Many species of Conyza are also perennial rarely shrubs, growing to 1-2 m tall. The native area of the weed is warm temperate and tropical regions throughout the world (Paula et al, 2017). This weed has multi-stems near base and these stems are full of long white hairs.…”

Section: Introductionmentioning

confidence: 99%

“…This weed has multi-stems near base and these stems are full of long white hairs. Leaves are thin, narrow, stalkless and become further smaller towards the top of plant (Santos et al, 2014;Paula et al, 2017). Inflorescence is branched and nearly 35 cm long and produces pale yellow flowers in September-October on short, thickly hairy pedicels.…”

Section: Introductionmentioning

confidence: 99%

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Optimizing efficacy of acetochlor + atrazine and dicamba at various doses to manage Conyza stricta L. in sugarcane

et al. 2020

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“…These modifications play a controlled role in regulating the expression of neighboring genes [9,10]. Consequently, without altering the DNA sequence, they have a profound influence on leaf development and morphogenesis [11][12][13][14].…”

mentioning

confidence: 99%

Heritable Epigenetic Modification of BpIAA9 Causes the Reversion Mutation of Leaf Shapes in<em> Betula pendula</em> ‘Dalecarlica’

Zhang,

Gu,

Jiang

et al. 2023

Preprint

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The European white birch, scientifically known as Betula pendula, and its variant, B. pendula 'Dalecarlica' are characterized by a serrated leaf margin that enhances their aesthetic appeal. However, the lobed leaf trait can undergo random and spontaneous reversion to the typical ovoid or cordate shape during asexual reproduction. Investigating and elucidating the molecular mechanisms underlying this unpredictable reversion mutation is essential for comprehending the birch leaf development process. In this research, we employed a non-lobed-leaf mutant derived from a lobed-leaf birch clone during plant tissue culture. We adopted a multi-omics approach, including whole-genome resequencing, transcriptome sequencing, and methylation profiling, to analyze and compare genomic variations and gene expression modifications. The study revealed that the 24 variant genes affected by 1464 SNP/InDel sites in the genome of the non-lobed-leaf mutant are not associated with leaf development. While the overall methylation level in the mutant's genome closely resembles that of the serrated-leaf birch, ten genes exhibit differential methylation accompanied by differential expression. Transcriptome sequencing demonstrated that the differentially downregulated genes in the mutant are significantly enriched in the GO:0009733 (response to auxin) and GO:0009734 (auxin-activated signaling) pathways. Validation through McrBC-PCR and qRT-PCR confirmed differential methylation and expression of BpIAA9 in the reversion mutant. The elevated methylation level in the BpIAA9 promoter leads to reduced expression, resulting in changes in the expression of auxin-responsive genes. This, in turn, leads to a transcriptional downregulation enrichment effect in auxin-related pathways in the reversion mutant, ultimately inhibiting the regulation of leaf veins by auxin during their development.

show abstract

Morphological analysis and DNA methylation in Conyza bonariensis L. cronquist (Asteraceae) phenotypes

Cited by 5 publications

References 31 publications

Heritable Epigenetic Modification of BpIAA9 Causes the Reversion Mutation of Leaf Shapes in Betula pendula ‘Dalecarlica’

Heritable Epigenetic Modification of BpIAA9 Causes the Reversion Mutation of Leaf Shapes in Betula pendula ‘Dalecarlica’

Optimizing efficacy of acetochlor + atrazine and dicamba at various doses to manage Conyza stricta L. in sugarcane

Heritable Epigenetic Modification of BpIAA9 Causes the Reversion Mutation of Leaf Shapes in<em> Betula pendula</em> ‘Dalecarlica’

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