Morphofunctional evaluation of end-to-side neurorrhaphy through video system magnification

Barros, Rui Sergio Monteiro de; Brito, Marcus Vinicius Henriques; Brito, Marcelo Houat de; Coutinho, Jean Vitor de Aguiar Lédo; Teixeira, Renan Kleber Costa; Yamaki, Vítor Nagai; Costa, Felipe Lobato da Silva; Somensi, Danusa Neves

doi:10.1016/j.jss.2017.08.003

Cited by 13 publications

(23 citation statements)

References 17 publications

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“…The behavioral and functional assessment of peripheral nerve regeneration are simple tests that could score the grade of injury/recovery of motor function 12 , 15 , 16 . These assets are no invasive procedures, then could be applied several times different than histological and electrophysiological parameters 20 , 21 . Nonetheless, a researcher must be trained in how to do the test, before applying in research to avoid misinterpretation, as was identified in this paper where some normal animals need to execute more than three times one test to have the real score.…”

Section: Discussionmentioning

confidence: 99%

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Criteria for assessing peripheral nerve injury. Behavioral and functional assessment in non-operated Wistar rats

et al. 2020

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Section: Discussionmentioning

confidence: 99%

“…These tests do not evaluate reflex acts, but complex motor acts involving agonist and antagonist muscles 11 , 13 , 16 . And when there is an injury to a particular peripheral nerve, these quantify and qualify the degree of loss and allow to assess whether there was a functional recovery 20 , 21 .…”

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confidence: 99%

Criteria for assessing peripheral nerve injury. Behavioral and functional assessment in non-operated Wistar rats

et al. 2020

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“…Specific instruments and materials were used to perform anastomoses and dissections. The surgical procedures were performed using a video system 16,17 consisting of a high-definition Sony Handycam HDR-XR160 set to Â50 magnification, a lightemitting diode high-definition 55-inch television, and a digital HDMI cable. Two low-intensity halogen light sources were used near the camera to provide adequate illumination of the operative field.…”

Section: Methodsmentioning

confidence: 99%

Novel Porcine Kidney-Based Microsurgery Training Model for Developing Basic to Advanced Microsurgical Skills

Reis

Teixeira

Santos

et al. 2020

J Reconstr Microsurg

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Background Microsurgery training is critical to the practice of microvascular procedures in many surgical areas. However, even simple procedures require different levels of complex skills. Therefore, simulation-based surgical training, mainly in the area of vascular anastomosis, is of great importance. In this paper, we present a new microsurgery training model for the development of basic to advanced microsurgical skills. Methods Porcine kidneys were purchased from a legal butchery slaughterhouse. First, kidneys were washed with water to remove blood and clots inside vessels. Then, dissection was performed throughout the vascular pedicle from the renal arteries to the segmentary branches. Finally, the longitudinal sectioning of the kidney parenchyma was performed to expose the vessels necessary for training. Sixty end-to-end anastomoses were performed. Specific instruments and materials were used to perform anastomoses and dissections with magnification by a video system. We evaluated the diameter of vessels, time to perform anastomosis, and patency of anastomosis. Results There was no great anatomical variation among the porcine kidneys. The total length for dissection training was 25.80 ± 7.44 cm using the arterial and venous vessel. The average time to perform arterial anastomoses was 23.79 ± 4.55 minutes. For vessel diameters of ≤ 3, 4 to 6, and 7 to 10 mm, the average procedure times were 27.68 ± 3.39, 22.92 ± 4.12, and 20.77 ± 3.44 minutes, respectively. Regarding venous anastomosis, the average duration of the procedure was 26.17 ± 4.80 minutes, including durations of 31.61 ± 3.86, 25.66 ± 4.19, and 21.24 ± 3.79 minutes for vessel diameters of ≤ 7, 8 to 10, and >10 mm, respectively. Positive patency was achieved in all surgeries. Conclusion The porcine kidney provides an inexpensive and convenient biological model for modeling microanastomosis with high fidelity to vascular structures.

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“…The temperature was set on 24ºC. The electrophysiologic testing protocol was the following: 1) The rats were anesthetized with intraperitoneal ketamine (70 mg/kg) and xylazine (10 mg/kg) 8 , 9 and then shaved and placed in a horizontal supine position. 2) The temperature was measured with infrared thermometer at the time of examination.…”

Section: Methodsmentioning

confidence: 99%

“…4) All animals were submitted to two electroneuromyography analysis: first with surface electrode and then by needle electrode; by a Neuropack S1 MEB- 9400 © (Nihon Kohden, Japan). 5) For each compound muscle action potential, the latency, amplitude, duration and area of the negative peak of the gastrocnemius and cranial tibial muscles were measured and recorded 5 , 8 , 9 .…”

Section: Methodsmentioning

confidence: 99%

Does the type of electrode affect the electromyoneurographic parameters in rats?

et al. 2019

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Purpose To evaluate if the type of electrode (needle vs. surface) affects the electromyoneurography parameters in rats. Methods Twenty male rats were anesthetized, then compound muscle action potential were recorded using a Neuropack S1 MEB- 9400 © . All animals were submitted to two electroneuromyography analysis: first with surface electrode and then by needle electrode. We evaluated the latency, amplitude, duration and area of the negative peak of the gastrocnemius and cranial tibial muscles. Results There were no significant differences between the groups in the mean of duration, latency, amplitude or area of the negative peak in gastrocnemius and cranial tibial muscles. Conclusion The type of electrode does not affect the electroneuromyography parameters.

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Morphofunctional evaluation of end-to-side neurorrhaphy through video system magnification

Cited by 13 publications

References 17 publications

Criteria for assessing peripheral nerve injury. Behavioral and functional assessment in non-operated Wistar rats

Criteria for assessing peripheral nerve injury. Behavioral and functional assessment in non-operated Wistar rats

Novel Porcine Kidney-Based Microsurgery Training Model for Developing Basic to Advanced Microsurgical Skills

Does the type of electrode affect the electromyoneurographic parameters in rats?

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