Morphoagronomic characterization and genetic diversity of a common bean RIL mapping population derived from the cross Rudá x AND 277

Abstract

ABSTRACT. Recombinant inbred lines (RILs) are a valuable resource for building genetic linkage maps. The presence of genetic variability in the RILs is essential for detecting associations between molecular markers and loci controlling agronomic traits of interest. The main goal of this study was to quantify the genetic diversity of a common bean 2 L.C. Silva et al. Genetics and Molecular Research 15 (3): gmr.15038112 RIL population derived from a cross between Rudá (Mesoamerican gene pool) and AND 277 (Andean gene pool). This population was developed by the single seed descent method from 500 F 2 plants until the F 10 generation. Seven quantitative traits were evaluated in the field in 393 RILs, the parental lines, and five control cultivars. The plants were grown using a randomized block design with additional controls and three replicates. Significant differences were observed among the RILs for all evaluated traits (P < 0.01). A comparison of the RILs and parental lines showed significant differences (P < 0.01) for the number of days to flowering (DFL) and to harvest (DH), productivity (PROD) and mass of 100 beans (M100); however, there were no significant differences for plant architecture, degree of seed flatness, or seed shape. These results indicate the occurrence of additive x additive epistatic interactions for DFL, DH, PROD, and M100. The 393 RILs were shown to fall into 10 clusters using Tocher's method. This RIL population clearly contained genetic variability for the evaluated traits, and this variability will be crucial for future studies involving genetic mapping and quantitative trait locus identification and analysis.

Assessment of the genetic diversity of cultivated common beans (Phaseolus vulgaris L.) from Cameroon and Kenya using allozymes markers

“…Characterization of crop germplasm using genetic markers provides estimates of genetic diversity, information which is essential for a rational utilization of genetic resources in breeding programs. Conventionally, plant genetic diversity is estimated using variations in morphoagronomics traits such as yield, resistance, tolerance, colour and size (Szilagyi et al, 2011;Silva et al, 2016). Recently, biochemical markers such as allozymes (Kouam et al, 2012;Pasquet, 1999) and DNA based markers (Coulibaly et al, 2002;Semagn et al, 2006;Adesoye & Ojobo, 2012) have been more frequently used.…”