More than just a barrier: using physical models to couple membrane shape to cell function

Frey, Felix J.; Idema, Timon

doi:10.1039/d0sm01758b

Cited by 22 publications

(21 citation statements)

References 207 publications

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“…It has been suggested theoretically that the shape of lipid bilayer vesicles is given by their minimal energy configuration [35,39,58]. Moreover, it has been shown that by increasing the preferred curvature and decreasing the reduced volume, vesicles can get divided [30,32].…”

Section: Discussionmentioning

confidence: 99%

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Membrane area gain and loss during cytokinesis

Frey

Idema²

2022

Phys. Rev. E

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In cytokinesis of animal cells, the cell is symmetrically divided into two. Since the cell's volume is conserved, the projected area has to increase to allow for the change of shape. Here we aim to predict how membrane gain and loss adapt during cytokinesis. We work with a kinetic model in which membrane turnover depends on membrane tension and cell shape. We apply this model to a series of calculated vesicle shapes as a proxy for the shape of dividing cells. We find that the ratio of kinetic turnover parameters changes nonmonotonically with cell shape, determined by the dependence of exocytosis and endocytosis on membrane curvature. Our results imply that controlling membrane turnover will be crucial for the successful division of artificial cells.

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Section: Discussionmentioning

confidence: 99%

“…In this paper, we theoretically investigate the interplay between shape changes and membrane trafficking during cytokinesis, following the assumption that the interior of the cell can be inferred from modeling membrane deformations [35]. Our analysis consists of two steps.…”

Section: Introductionmentioning

confidence: 99%

Membrane area gain and loss during cytokinesis

Frey

Idema²

2022

Phys. Rev. E

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“…Unlike other approaches 72 , Ψ does not allow for a direct control of the bilayer curvature but is rather an indirect mechanism analogous to the protein-membrane surface interplay, as previously described for α-synuclein 13,36 and the N-BAR domain 52 , both known for inducing curvature in lipid bilayers 57,74 . Besides, the complex saddle-shape mode in which the bilayer bends follows two principal curvatures defined by two fitted circles with different radii 3,4,22 and requires significantly more energy than other modes of simpler geometry.…”

Section: Segregation and Opening Are Coupled Eventsmentioning

confidence: 99%

Cholesterol plays a decisive role in tetraspanin assemblies during bilayer deformations

Caparotta

Masone

2021

Preprint

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The tetraspanin family plays key roles in many physiological processes, such as, tumour invasion, cell motility, virus infection, cell attachment and entry. Tetraspanins function as molecular scaffolds organised in microdomains with interesting downstream cellular consequences. However, despite their relevance in human physiology, the precise mechanisms of their various functions remain elusive. In particular, the full-length CD81 tetraspanin has interesting cholesterol-related properties that modulate its activity in cells. In this work, we study the opening transition of CD81 under different conditions. We propose that such conformational change is a collaborative process enhanced by simultaneous interactions between multiple identical CD81 tetraspanins. With molecular dynamics simulations we describe the crucial role of a ternary lipid bilayer with cholesterol in CD81 conformational dynamics, observing two emergent properties: first, clusters of CD81 collectively segregate one tetraspanin while favouring one opening transition, second, cumulative cholesterol sequestering by CD81 tetraspanins inhibits large membrane deformations due to local density variations.

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“…Micro-and nano-particles interacting with cell membranes made of lipid bilayers may induce membrane poration, rupture, particle engulfment or simply repulsion [1][2][3][4]. Hence, understanding and controlling particle-lipid membrane interaction is crucial for drug delivery, microbial infection, toxicity from microplastics and in many other fields [1].…”

Section: Introductionmentioning

confidence: 99%

Driven Engulfment of Janus Particles by Giant Vesicles in and out of Thermal Equilibrium

2022

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The interaction between Janus colloids and giant lipid vesicles was experimentally investigated to elucidate the dynamics and mechanisms related to microparticle engulfment by lipid vesicles. Janus (Pt–SiO2 and Pt–MF, where MF is melamine formaldehyde) colloids do not spontaneously adhere to POPC or DOPC bilayers, but by applying external forces via centrifugation we were able to force the contact between the particles and the membranes, which may result in a partial engulfment state of the particle. Surface properties of the Janus colloids play a crucial role in the driven particle engulfment by vesicles. Engulfment of the silica and platinum regions of the Janus particles can be observed, whereas the polymer (MF) region does not show any affinity towards the lipid bilayer. By using fluorescence microscopy, we were able to monitor the particle orientation and measure the rotational dynamics of a single Janus particle engulfed by a vesicle. By adding hydrogen peroxide to the solution, particle self-propulsion was used to perform an active transport of a giant vesicle by a single active particle. Finally, we observe that partially engulfed particles experience a membrane curvature-induced force, which pushes the colloids towards the bottom where the membrane curvature is the lowest.

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More than just a barrier: using physical models to couple membrane shape to cell function

Abstract: Physical models can help us to infer, from the shape of the outer membrane, which biological processes happen inside the living cell.

Cited by 22 publications

References 207 publications

Membrane area gain and loss during cytokinesis

Membrane area gain and loss during cytokinesis

Cholesterol plays a decisive role in tetraspanin assemblies during bilayer deformations

Driven Engulfment of Janus Particles by Giant Vesicles in and out of Thermal Equilibrium

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