Morbidity, Mortality, and Reliability of Accounting of Patients of Different Age Groups with Liver Cancer in Russia

Мерабишвили, В. М.; Gurkalo, V. K.; Мерабишвили, Э. Н.

doi:10.1134/s2079057020040141

Cited by 1 publication

(1 citation statement)

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“…PC mortality rate is strongly correlated to the stage of the disease when first diagnosed. According to the St. Petersburg population-based cancer registry, the five-year relative survival rate of PC patients drops dramatically with increasing disease stage: 100% (stage I), 100% (stage II), 69% (stage III), and only 24% (stage IV) [ 1 ]. Data arising from other sources including the United States Surveillance, Epidemiology, and End Results (SEER) database demonstrate a similar tendency.…”

Section: Introductionmentioning

confidence: 99%

A New Approach for Prostate Cancer Diagnosis by miRNA Profiling of Prostate-Derived Plasma Small Extracellular Vesicles

Zabegina¹,

Nazarova²,

Nikiforova³

et al. 2021

Cells

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Vesicular miRNA has emerged as a promising marker for various types of cancer, including prostate cancer (PC). In the advanced stage of PC, the cancer-cell-derived small extracellular vesicles (SEVs) may constitute a significant portion of circulating vesicles and may mediate a detectable change in the plasma vesicular miRNA profile. However, SEVs secreted by small tumor in the prostate gland constitute a tiny fraction of circulating vesicles and cause undetectable miRNA pattern changes. Thus, the isolation and miRNA profiling of a specific prostate-derived fraction of SEVs can improve the diagnostic potency of the methods based on vesicular miRNA analysis. Prostate-specific membrane antigen (PSMA) was selected as a marker of prostate-derived SEVs. Super-paramagnetic beads (SPMBs) were functionalized by PSMA-binding DNA aptamer (PSMA–Apt) via a click reaction. The efficacy of SPMB–PSMA–Apt complex formation and PSMA(+)SEVs capture were assayed by flow cytometry. miRNA was isolated from the total population of SEVs and PSMA(+)SEVs of PC patients (n = 55) and healthy donors (n = 30). Four PC-related miRNAs (miR-145, miR-451a, miR-143, and miR-221) were assayed by RT-PCR. The click chemistry allowed fixing DNA aptamers onto the surface of SPMB with an efficacy of up to 89.9%. The developed method more effectively isolates PSMA(+)SEVs than relevant antibody-based technology. The analysis of PC-related miRNA in the fraction of PSMA(+)SEVs was more sensitive and revealed distinct diagnostic potency (AUC: miR-145, 0.76; miR-221, 0.7; miR-451a, 0.65; and miR-141, 0.64) than analysis of the total SEV population. Thus, isolation of prostate-specific SEVs followed by analysis of vesicular miRNA might be a promising PC diagnosis method.

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Section: Introductionmentioning

confidence: 99%