Monophyletic group of unclassified γ- Proteobacteria dominates in mixed culture biofilm of high-performing oxygen reducing biocathode

Rothballer, Michael; Picot, Matthieu; Sieper, Tina; Arends, Jan; Schmid, Michael; Hartmann, Anton; Boon, Nico; Buisman, Cees J.N.; Barrière, Frédéric; Strik, D.P.B.T.B.

doi:10.1016/j.bioelechem.2015.04.004

Cited by 50 publications

(36 citation statements)

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“…from biomass degradation) and cyanobacteria via oxidation of stored organic carbon (Yagishita et al 1993). The cathodic current observed during illumination was previously shown to be the result of cathodic oxygen reduction and correlated to the presence of gamma-proteobacteria (Darus et al 2015;Rothballer et al 2014) (see Graphical abstract).…”

Section: Microbial Communitymentioning

confidence: 93%

Marine phototrophic consortia transfer electrons to electrodes in response to reductive stress

et al. 2015

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This work studies how extracellular electron transfer (EET) from cyanobacteria-dominated marine microbial biofilms to solid electrodes is affected by the availability of inorganic carbon (Ci). The EET was recorded chronoamperometrically in the form of electrical current by a potentiostat in two identical photo-electrochemical cells using carbon electrodes poised at a potential of +0.6 V versus standard hydrogen electrode under 12/12 h illumination/dark cycles. The Ci was supplied by the addition of NaHCO3 to the medium and/or by sparging CO2 gas. At high Ci conditions, EET from the microbial biofilm to the electrodes was observed only during the dark phase, indicating the occurrence of a form of night-time respiration that can use insoluble electrodes as the terminal electron acceptor. At low or no Ci conditions, however, EET also occurred during illumination suggesting that, in the absence of their natural electron acceptor, some cyanobacteria are able to utilise solid electrodes as an electron sink. This may be a natural survival mechanism for cyanobacteria to maintain redox balance in environments with limiting CO2 and/or high light intensity.

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Section: Microbial Communitymentioning

confidence: 93%

Marine phototrophic consortia transfer electrons to electrodes in response to reductive stress

et al. 2015

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“…The FISH-method as described in Manz et al (1992) and Amann et al (1991) was applied modified for root samples as described in Rothballer et al (2015). For A. radicis N35 the specific probe ACISP 145 (TTTCGCTCCGTTATCCCC), combined with an equimolar mixture of the universal bacterial probes EUB 338 I (GCTGCCTCCCGTAGGA), EUB 338 II (GCAGCCACCCGTAGGTGT), and EUB 338 III (GCTGCCACCCGTAGGTGT) were used.…”

Section: Methodsmentioning

confidence: 99%

Systemic Responses of Barley to the 3-hydroxy-decanoyl-homoserine Lactone Producing Plant Beneficial Endophyte Acidovorax radicis N35

Han

Trost

et al. 2016

Front. Plant Sci.

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Quorum sensing auto-inducers of the N-acyl homoserine lactone (AHL) type produced by Gram-negative bacteria have different effects on plants including stimulation on root growth and/or priming or acquirement of systemic resistance in plants. In this communication the influence of AHL production of the plant growth promoting endophytic rhizosphere bacterium Acidovorax radicis N35 on barley seedlings was investigated. A. radicis N35 produces 3-hydroxy-C10-homoserine lactone (3-OH-C10-HSL) as the major AHL compound. To study the influence of this QS autoinducer on the interaction with barley, the araI-biosynthesis gene was deleted. The comparison of inoculation effects of the A. radicis N35 wild type and the araI mutant resulted in remarkable differences. While the N35 wild type colonized plant roots effectively in microcolonies, the araI mutant occurred at the root surface as single cells. Furthermore, in a mixed inoculum the wild type was much more prevalent in colonization than the araI mutant documenting that the araI mutation affected root colonization. Nevertheless, a significant plant growth promoting effect could be shown after inoculation of barley with the wild type and the araI mutant in soil after 2 months cultivation. While A. radicis N35 wild type showed only a very weak induction of early defense responses in plant RNA expression analysis, the araI mutant caused increased expression of flavonoid biosynthesis genes. This was corroborated by the accumulation of several flavonoid compounds such as saponarin and lutonarin in leaves of root inoculated barley seedlings. Thus, although the exact role of the flavonoids in this plant response is not clear yet, it can be concluded, that the synthesis of AHLs by A. radicis has implications on the perception by the host plant barley and thereby contributes to the establishment and function of the bacteria-plant interaction.

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“…Great attention has been paid to microbial biocathodes due to their advantages, and both mixed microbial communities and pure cultures have been reported to catalyze ORR. Mixed microbial communities give broad bacterial diversity, and can be derived from seawater [5,6], freshwater [7], and wasterwater treatment sludge [8][9][10]. Although mixed microbial communities display robust catalysis efficiency towards ORR, their complex multispecies nature brings great difficulty for mechanism investigation, which stimulates the research of ORR catalyzed by pure cultures.…”

Section: Introductionmentioning

confidence: 99%

Catalysis of oxygen reduction reaction by an iron-reducing bacterium isolated from marine corrosion product layers

Wang

Zhang

et al. 2016

Journal of Electroanalytical Chemistry

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Monophyletic group of unclassified γ- Proteobacteria dominates in mixed culture biofilm of high-performing oxygen reducing biocathode

Cited by 50 publications

References 50 publications

Marine phototrophic consortia transfer electrons to electrodes in response to reductive stress

Marine phototrophic consortia transfer electrons to electrodes in response to reductive stress

Systemic Responses of Barley to the 3-hydroxy-decanoyl-homoserine Lactone Producing Plant Beneficial Endophyte Acidovorax radicis N35

Catalysis of oxygen reduction reaction by an iron-reducing bacterium isolated from marine corrosion product layers

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