Monocytes Subpopulations and Chemiluminescent Activity in Patients With Renal Cell Carcinoma

Савченко, А. А.; Борисов, А Г; Модестов, А А; Moshev, A. V.; Владимирович, Кудрявцев Игорь; Tonacheva, O. G.; Koshcheev, V. N.

doi:10.15789/1563-0625-2015-2-141-150

Cited by 9 publications

(5 citation statements)

References 21 publications

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“…На их поверхности активно экспрессируются CX3CR1-, CD11c- и HLA-DR-молекулы, тогда как CD62L и CD64 практически отсутствует. Данная субпопуляция активно синтезирует провоспалительные цитокины (TNFα, IL-1β и IL-6) и определяется как провоспалительная [6,12]. Необходимо отметить, что повышение содержания неклассических моноцитов отмечено при различных инфекционно-воспалительных заболеваниях [12,34].…”

Section: Discussionunclassified

“…Однако необходимо отметить, что и сами моноциты, также как и их потомки -макрофаги и дендритные (стимулирующие развитие адаптивного иммунного ответа в качестве антигенпрезентирующих клеток), являются регуляторными клетками, в том числе, и для клеток врожденного иммунитета [2,8,24]. К тому же моноциты, долгое время рассматривающиеся в качестве моноклеточной популяции, на сегодняшний день, в зависимости от экспрессии CD16рецептора, делятся на две основные субпопуляции: классические моноциты (CD14 + CD16 -) и неклас-сические (CD14 + CD16 + ) [1,6,16]. При этом регуляторная роль субпопуляций моноцитов на гранулоцитарные клетки крови при РГП до сих пор не изучена.…”

Section: Introductionunclassified

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Regulatory Influence of Blood Monocytes on the Population Composition of Granulocytes and the State of Their Respiratory Burst in the Widespread Purulent Peritonitis

Савченко¹,

Борисов²,

Черданцев³

et al. 2018

Russian Journal of Infection and Immunity

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The aim of the study was to investigate the regulatory effect of monocytes and their subpopulations on the population composition of granulocyte leukocytes and the state of their respiratory burst in widespread purulent peritonitis (WPP). The study involved 24 patients aged 30-65 with acute surgical diseases and injuries of abdominal organs complicated by WPP. As a control 25 relatively healthy people of the same age range were examined. A study of the population composition of monocytes and granulocyte leukocytes in blood was performed using a two-platform technology on the hematological analyzer Sysmex XE-5000 (Sysmex Inc., USA) and FC-500 flow cytometer (Beckman Coulter, USA) using the Cytodiff antibody kit (Beckman Coulter, USA). A study of the monocytes number expressing HLA-DR- and CD64-receptor was performed by flow cytometry using direct immunofluorescence of whole peripheral blood. The respiratory burst state of neutrophilic granulocytes was studied by chemiluminescence analysis on a 36-channel chemiluminescence analyzer BLM-3607 (MedBioTech, Russia). As indicators of chemiluminescence were used luminol and lucigenin. The enhancement of chemiluminescence induced by zymosan was evaluated by the ratio of the area of the induced chemiluminescence to the spontaneous area and was defined as the activation index. It has been established that the immune-inflammatory process in WPP is characterized by a decrease in the number of classical monocytes in the peripheral blood and an increase in the content of non-classical monocytes. In WPP in peripheral blood the level of monocytes expressing HLA-DR receptors decreases. The change in the ratio of monocytes subpopulations characterizes the increase in the role of the proinflammatory fraction in the WPP pathogenesis. Changes in the population composition of granulocytes in the blood in patients with WPP also characterize the development of an acute inflammatory process. In this case, there is a decrease in the number of basophils in the peripheral blood, which, apparently, is determined by the presence of an allergic component in WPP and, accordingly, their migration to the inflammation area. In patients with WPP activation of a respiratory burst of granulocytes of blood was detected, the intensity of which is determined by the synthesis of primary and secondary active oxygen species. The results of the correlation analysis made it possible to establish that in WPP the regulatory role of non-classical monocytes increases aimed at stimulating the inflammatory processes (an increase in the number of mature and immature forms of neutrophils and stimulation of the activity of a respiratory explosion of granulocytes). The revealed features of the regulatory effect of monocytes on the population composition and the intensity of the respiratory burst of granulocytes can be used in the development of immunotherapeutic methods aimed at reducing the activity of the inflammatory process in WPP.

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Section: Discussionunclassified

Section: Introductionunclassified

Regulatory Influence of Blood Monocytes on the Population Composition of Granulocytes and the State of Their Respiratory Burst in the Widespread Purulent Peritonitis

Савченко¹,

Борисов²,

Черданцев³

et al. 2018

Russian Journal of Infection and Immunity

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“…CD16-антиген является низкоаффинным Fcрецептором для IgG [9,14,19]. Моноциты, не экспрессирующие CD16-антиген, определяются как «классические» [5,8,18]. Данная фракция представлена крупными клетками с высоким уровнем фагоцитарной активности.…”

Section: Discussionunclassified

Blood Leukocytes Phenotyping by Hematoflow Method in Patients With Onychomycosis

Савченко

Борисов

Анисимова

et al. 2016

Russian Journal of Infection and Immunity

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Инфекция и иммунитет А.А. Савченко и др. врожденного и адаптивного иммунитета. Он позволяет оценивать степень тяжести иммунопатологического процесса, механизм и уровень повреждения иммунной системы, можно рекомендовать его применение для персонифицированного подхода к назначению иммунотропного лечения.

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“…ДК, дифференцированные из моноцитов крови больных раком почки (РП), характеризовались сниженной костимуляторной и антигенпрезентирующей активностью за счет пониженного уровня экспрессии CD83 и CD86 [4]. У больных РП в крови нарушается соотношение моноцитарных субпопуляций, наблюдается дисбаланс экспрессии активационных молекул и снижается активность респираторного взрыва моноцитов [5]. Более того, в ряде работ повышение уровня провоспалительных моноцитов (CD14 + CD16 + ) в циркуляции связывают с увеличением содержания TAM и усилением роста опухоли [16,30].…”

Section: дендритные клетки при раке почкиunclassified

Interdependence Between the Phenotype of Dendritic Cells and Amounts of Blood Proinflammatory Monocytes in Patients With Kidney Cancer

et al. 2019

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The aim of the study was to investigate an interdependence between the phenotype of dendritic cells (DC) differentiated from monocytes and the number of pro-inflammatory monocytes in peripheral blood of patients with kidney cancer (KC). The study involved 28 patients at the age of 40-55 years suffering with KC (Т3N0М0, clear cell type) before surgical treatment. The diagnosis was verified histologically. 31 healthy agematched persons were examined as a control group. Mononuclear cells were isolated from heparinized venous blood by centrifugation in a Histopaque®-1077 density gradient followed by plastic adsorption in RPMI 1640 medium supplied with 10% autologous serum. Immature DCs (iDCs) were generated from blood monocytes by culturing for 5 days with GM-CSF and IFNα. Activation of DCs (mDCs) was induced by incubation with the tumor cell lysate and TNFα, followed by incubation for 48 hours. A tumor fragment was used to prepare the lysate of autologous tumor cells. Phenotyping of blood monocytes and DC at various maturation stages was performed by flow cytometry. The numbers of CD14+CD16+ monocytes in peripheral blood of KC patients were decreased (up to 42% of the total monocyte level) against the control ranges. In this regard, the analysis of the dependence between the phenotype of DCs differentiated from monocytes and the number of pro-inflammatory blood monocytes was carried out by comparing the groups with a high content of pro-inflammatory monocytes in the blood in KC patients (> 42%, near-control range) and low content (resp., < 42%). We have found that the contents of tolerogenic iDC in cell culture are increased in KC patients with low amounts of pro-inflammatory monocytes in blood (< 42%). A relatively increased expression of antigen-presenting and co-stimulatory molecules proved to be the specific feature of iDC phenotype in patients with high contents (> 42%) of proinflammatory monocytes in blood. The phenotype of dendritic cells in KC patients with different content of proinflammatory monocytes during maturation/activation showed more differences. In the patients with low levels of pro-inflammatory monocytes, the cell pool of in vitro maturing DCs was characterized by low level of CD86 and HLA-DR receptor expression, thus reflecting a weak co-stimulating and antigen-presenting activity. In the patients with high levels of pro-inflammatory monocytes in blood, the in vitro activated DCs showed higher level of functional activity using the above markers. The revealed differences in the DC phenotype and interrelations with amounts of blood monocyte subpopulations in KC patients may presume the programmed cell differentiation mechanisms depending on the microenvironment, under pathogenic conditions (i.e., in presence of malignant tumor growth).

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Monocytes Subpopulations and Chemiluminescent Activity in Patients With Renal Cell Carcinoma

Cited by 9 publications

References 21 publications

Regulatory Influence of Blood Monocytes on the Population Composition of Granulocytes and the State of Their Respiratory Burst in the Widespread Purulent Peritonitis

Regulatory Influence of Blood Monocytes on the Population Composition of Granulocytes and the State of Their Respiratory Burst in the Widespread Purulent Peritonitis

Blood Leukocytes Phenotyping by Hematoflow Method in Patients With Onychomycosis

Interdependence Between the Phenotype of Dendritic Cells and Amounts of Blood Proinflammatory Monocytes in Patients With Kidney Cancer

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