Monocyte Phenotype and Cytokine Production Profiles Are Dysregulated by Short-Duration Spaceflight

Crucian, Brian; Stowe, Raymond P.; Quiriarte, Heather; Pierson, Duane L.; Sams, Clarence F.

doi:10.3357/asem.3047.2011

Cited by 85 publications

(82 citation statements)

References 26 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Additionally, because of their ability to colonize the human body and produce toxins, volatile organic compounds, and proteases, the common molds ( Alternaria , Cladosporium , Epicoccum ) could damage airways of immunocompromised occupants, which make them more dangerous than any other allergenic source [82–85]. In this study, accumulation of Alternaria and Epicoccum genera over time was observed, which, in combination with reported decreased immunity in occupants of confined spaces, e.g., astronauts [50, 86] could lead to developing allergy and asthma symptoms.…”

Section: Discussionmentioning

confidence: 73%

Human presence impacts fungal diversity of inflated lunar/Mars analog habitat

et al. 2017

View full text Add to dashboard Cite

BackgroundAn inflatable lunar/Mars analog habitat (ILMAH), simulated closed system isolated by HEPA filtration, mimics International Space Station (ISS) conditions and future human habitation on other planets except for the exchange of air between outdoor and indoor environments. The ILMAH was primarily commissioned to measure physiological, psychological, and immunological characteristics of human inhabiting in isolation, but it was also available for other studies such as examining its microbiological aspects. Characterizing and understanding possible changes and succession of fungal species is of high importance since fungi are not only hazardous to inhabitants but also deteriorate the habitats. Observing the mycobiome changes in the presence of human will enable developing appropriate countermeasures with reference to crew health in a future closed habitat.ResultsSuccession of fungi was characterized utilizing both traditional and state-of-the-art molecular techniques during the 30-day human occupation of the ILMAH. Surface samples were collected at various time points and locations to observe both the total and viable fungal populations of common environmental and opportunistic pathogenic species. To estimate the cultivable fungal population, potato dextrose agar plate counts method was utilized. The internal transcribed spacer region-based iTag Illumina sequencing was employed to measure the community structure and fluctuation of the mycobiome over time in various locations. Treatment of samples with propidium monoazide (PMA; a DNA intercalating dye for selective detection of viable microbial populations) had a significant effect on the microbial diversity compared to non-PMA-treated samples. Statistical analysis confirmed that viable fungal community structure changed (increase in diversity and decrease in fungal burden) over the occupation time. Samples collected at day 20 showed distinct fungal profiles from samples collected at any other time point (before or after). Viable fungal families like Davidiellaceae, Teratosphaeriaceae, Pleosporales, and Pleosporaceae were shown to increase during the occupation time.ConclusionsThe results of this study revealed that the overall fungal diversity in the closed habitat changed during human presence; therefore, it is crucial to properly maintain a closed habitat to preserve it from deteriorating and keep it safe for its inhabitants. Differences in community profiles were observed when statistically treated, especially of the mycobiome of samples collected at day 20. On a genus level Epiccocum, Alternaria, Pleosporales, Davidiella, and Cryptococcus showed increased abundance over the occupation time.Electronic supplementary materialThe online version of this article (doi:10.1186/s40168-017-0280-8) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 73%

Human presence impacts fungal diversity of inflated lunar/Mars analog habitat

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Many years later, another immune system disturbance was discovered exhibiting latent virus reactivation such as varicella zoster 7, 8 . Since the first evidences of a compromised immune system in space, sensitivity of cells of the human immune system to reduced gravity has been confirmed by numerous studies in real and simulated microgravity 9–15 . However, the mechanisms underlying gravity-regulated cellular responses are still unknown.…”

Section: Introductionmentioning

confidence: 94%

Dynamic gene expression response to altered gravity in human T cells

Thiel

Hauschild

Huge

et al. 2017

Sci Rep

100

View full text Add to dashboard Cite

We investigated the dynamics of immediate and initial gene expression response to different gravitational environments in human Jurkat T lymphocytic cells and compared expression profiles to identify potential gravity-regulated genes and adaptation processes. We used the Affymetrix GeneChip® Human Transcriptome Array 2.0 containing 44,699 protein coding genes and 22,829 non-protein coding genes and performed the experiments during a parabolic flight and a suborbital ballistic rocket mission to cross-validate gravity-regulated gene expression through independent research platforms and different sets of control experiments to exclude other factors than alteration of gravity. We found that gene expression in human T cells rapidly responded to altered gravity in the time frame of 20 s and 5 min. The initial response to microgravity involved mostly regulatory RNAs. We identified three gravity-regulated genes which could be cross-validated in both completely independent experiment missions: ATP6V1A/D, a vacuolar H + -ATPase (V-ATPase) responsible for acidification during bone resorption, IGHD3-3/IGHD3-10, diversity genes of the immunoglobulin heavy-chain locus participating in V(D)J recombination, and LINC00837, a long intergenic non-protein coding RNA. Due to the extensive and rapid alteration of gene expression associated with regulatory RNAs, we conclude that human cells are equipped with a robust and efficient adaptation potential when challenged with altered gravitational environments.

show abstract

“…Parabolic flight induces an increase in PMN number and the capability of PMNs to produce hydrogen peroxide (H 2 O 2 ) in response to soluble stimuli (Kaufmann et al 2009). These works seemingly indicate that the action of microgravity has enhanced the immune response of PMNs, which is inconsistent with the reduced capability of lymphocytes (Cogoli and Tschopp 1985;Konstantinova et al 1993;Meshkov and Rykova 1995;Mills et al 2002;Mills et al 2008Mills et al , 2013, monocytes (Kaur et al 2005(Kaur et al , 2008Crucian et al 2011), NK cells (Rykova et al 1992;Konstantinova et al 1993Konstantinova et al , 1995Mehta et al 2001), and macrophages (Armstrong et al 1995;Hsieh et al 2005;Ortega et al 2012;Adrian et al 2013;Wang et al 2014Wang et al , 2015Brungs et al 2015;Paulsen et al 2015) under microgravity. The underlying mechanisms for such the difference remain unclear.…”

Section: Introductionmentioning

confidence: 97%

Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells

Wang

Zhang

et al. 2015

Microgravity Sci. Technol.

View full text Add to dashboard Cite

Altered gravity, especially microgravity affects cellular functions of immune cells and can result in immune dysfunction for long-term, manned spaceflight and space exploration. The underlying mechanism, however, of sensing and responding to the gravity alteration is poorly understood. Here, a rotary cell culture system (RCCS) bioreactor was used to elucidate the effects of simulated microgravity on polymorphonuclear neutrophils (PMN)-like HL-60 cells. Alteration of cell morphology, up-regulation of (nitric oxide) NO production, enhancement of interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein 1 (MCP-1) secretion, and diversity of cellular adhesion molecule expression were observed for the cells cultured in RCCS, leading to the up-regulated inflammatory immune responses and host defense. It was also indicated that such alterations in biological responses of PMNs mediated the reduced rolling velocity and decreased adhesion of PMNlike HL-60 cells on endothelial cells under shear flow. This work furthers the understandings in the effects and mechanism of microgravity on PMN functions, which are Chengzhi Wang and Ning Li, both authors contributed equally to this work. Electronic supplementary material

show abstract

Monocyte Phenotype and Cytokine Production Profiles Are Dysregulated by Short-Duration Spaceflight

Cited by 85 publications

References 26 publications

Human presence impacts fungal diversity of inflated lunar/Mars analog habitat

Human presence impacts fungal diversity of inflated lunar/Mars analog habitat

Dynamic gene expression response to altered gravity in human T cells

Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells

Contact Info

Product

Resources

About