Monocyte inflammatory profile is specific for individuals and associated with altered blood lipid levels

Patel, Vyoma; Williams, Helen; Li, Stephen C.H.; Fletcher, J.; Medbury, Heather

doi:10.1016/j.atherosclerosis.2017.05.026

Cited by 51 publications

(61 citation statements)

References 48 publications

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“…*p < 0.05, **p < 0.01, ***p < 0.001, Student's unpaired t test. (2020) 10:4397 | https://doi.org/10.1038/s41598-020-61022-1 www.nature.com/scientificreports www.nature.com/scientificreports/ alterations, as well as environmentally linked changes in monocytes (with factors such as viral infections, microbiota composition, serum lipid levels, and lifestyle choices) [34][35][36][37][38][39][40][41][42][43] .…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Human Monocyte Subsets and Evidence for Phenotypic Groups Defined by Interindividual Variations of Expression of Adhesion Molecules

Merah-Mourah

Cohen

Charron

et al. 2020

Sci Rep

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Monocytes contribute to immune responses as a source for subsets of dendritic cells and macrophages. Human blood monocytes are classified as classical, non-classical and intermediate cells. However, the particular functions of these subsets have been hard to define, with conflicting results and significant overlaps. One likely reason for these ambiguities is in the heterogeneity of these monocyte subsets regrouping cells with divergent functions. To better define monocyte populations, we have analysed expression of 17 markers by multicolour flow cytometry in samples obtained from 28 control donors. Data acquisition was tailored to detect populations present at low frequencies. Our results reveal the existence of novel monocyte subsets detected as larger CD14 + cells that were CD16 + or CD16 neg. These large monocytes differed from regular, smaller monocytes with respect to expression of various cell surface molecules, such as FcR, chemokine receptors, and adhesion molecules. Unsupervised multidimensional analysis confirmed the existence of large monocytes and revealed interindividual variations that were grouped according to unique patterns of expression of adhesion molecules CD62L, CD49d, and CD43. Distinct inflammatory responses to TLR agonists were found in small and large monocytes. Overall, refining the definition of monocyte subsets should lead to the identification of populations with specific functions. Monocytes, which are mostly precursors of some macrophage and dendritic cell populations, have been hard to divide into populations with clear-cut inflammatory and immune functions. This may be due in part to the high number and complexity of phenotypes present in monocyte populations. A classification of human blood monocyte subsets based on the expression of CD14 and CD16 cell surface receptors was proposed 1 and refined over the years 2,3. It consisted initially of two populations described as "classical" monocytes, which express CD14 but no CD16, and "nonclassical" monocytes with low CD14 and strong CD16 expression. Further analysis identified CD14 + CD16 + monocytes with expression of CCR5 and intermediate expression of receptors divergently expressed in the two other subsets (e.g. CCR2 and CX3CR1 4. This additional subset was identified as "intermediate" 5. Evidence for this third subset was confirmed in transcriptome analysis 6-10. Despite progress in phenotypic analysis, immune functions associated with monocyte subpopulations in the steady state remained ill defined. Marked functional redundancies between the sub-populations were found, and contradicting results in the literature added to the puzzling difficulties in assigning functions to specific populations 11-13. Thus, intermediate monocytes were described as the major source of pro-inflammatory cytokines upon stimulation 14,15. In contrast, non-classical monocytes were also described as the most inflammatory monocytes 3,13,16. Similarly, anti-inflammatory cytokine secretion was alternatively found high in intermediate 13 or in classical monoc...

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Section: Discussionmentioning

confidence: 99%

Identification of Novel Human Monocyte Subsets and Evidence for Phenotypic Groups Defined by Interindividual Variations of Expression of Adhesion Molecules

Merah-Mourah

Cohen

Charron

et al. 2020

Sci Rep

View full text Add to dashboard Cite

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“…Yvan-Charvet et al [ 23 ] and Esteve et al [ 24 ] revealed the idea that the HDL levels are related to the cytokine levels. On the one hand, increased HDL levels can cause decreased circulating levels of proinflammatory cytokines, such as interleukin 6 (IL-6) and tumor necrosis factor (TNF-α) [ 25 ] . On the other hand, elevated HDL levels can produce increased levels of anti-inflammatory cytokines, such as IL-10 [ 26 ] .…”

Section: Discussionmentioning

confidence: 99%

Association of serum lipids and severity of epithelial ovarian cancer: an observational cohort study of 349 Chinese patients

Zhang

Liang

et al. 2018

J Biomed Res

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While obesity and fat intake have been associated with the risk and prognosis of epithelial ovarian cancer, the association between the lipid levels and epithelial ovarian cancer phenotype remains controversial. We conducted a retrospective study of 349 epithelial ovarian cancer patients who received treatment at Jiangsu Cancer Hospital, China between 2011 and 2017. We analyzed age at diagnosis, blood pressure, plasma glucose content, body mass index (BMI), lipid levels and clinical parameters. Severity of epithelial ovarian cancer was classified according to the International Federation of Gynecology and Obstetrics (FIGO) grading system. Univariate analysis of the clinical factors according to the severity of epithelial ovarian cancer was followed by logistic regression analysis to identify clinical factors significantly associated with epithelial ovarian cancer severity. Univariate analysis indicated that age, BMI, triglyceride (TG), and high density lipoproteins (HDL) differed significantly among different stages of epithelial ovarian cancer (P<0.05). In the logistic regression model, elevated TG (OR: 1.883; 95% CI= 1.207–2.937), and low HDL (OR: 0.497; 95% CI= 0.298–0.829) levels were significantly associated with the high severity epithelial ovarian cancer. Our data indicate that high TG and low HDL levels correlate with a high severity of epithelial ovarian cancer. These data provide important insight into the potential relationship between the lipid pathway and epithelial ovarian cancer phenotype and development.

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“…In response to TLR agonists, classical monocyte subsets are the most producers for TNFα, IL-6 and IL-1β and non-classical monocytes are the lowest producers [ 50 ]. Nevertheless, the classical monocytes produce high levels of IL-10 and low levels of TNF-α whereas intermediate monocytes mainly releasing TNF-α have been controversially reported [ 52 , 53 ]. In this study, mAb P-3E10 induced IL-6, IL-10 and TNF-α production in monocytes.…”

Section: Discussionmentioning

confidence: 99%

Ligation of Na, K ATPase β3 subunit on monocytes by a specific monoclonal antibody mediates T cell hypofunction

et al. 2018

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T cells play a crucial role in orchestrating body immune responses. T cell hyperfunction, however, leads to inflammation and induction of autoimmune diseases. Understanding of T cell regulation mechanisms and successful modulation of T cell responses is beneficial in treatment of disease associated to T cell hyperresponsiveness. Our previous study indicated that monoclonal antibody (mAb) P-3E10, a mAb to Na, K ATPase β3 subunit, inhibited anti-CD3-induced PBMC proliferation. In the current study, we further investigated the mechanism of mAb P-3E10 in the induction of T cell hypofunction. We demonstrated that mAb P-3E10 decreased T cell proliferation and Th1, Th2 and Th17 cytokine production. Monocytes were the cells playing a key role in mediation of mAb P-3E10 induced T cell hypofunction. The inhibition of T cell activation by mAb P-3E10 required cell contact between monocytes and T cells. The mAb P-3E10 induced the down-expression level of MHC class II and CD86 and increased IL-6, IL-10 and TNF-α production of monocytes. We concluded that ligation of the Na, K ATPase β3 subunit on monocytes by mAb P-3E10 arbitrated T cell hypofunction. This mAb might be a promising novel immunotherapeutic antibody for the treatment of hyperresponsive T cell associated diseases.

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Monocyte inflammatory profile is specific for individuals and associated with altered blood lipid levels

Abstract: Our data indicates that priming of all monocytes to an inflammatory state occurs in individuals with a perturbed lipid profile, overriding the normal functional distinction attributed to the different monocyte subsets. As such, all monocytes may be important in CVD.

Cited by 51 publications

References 48 publications

Identification of Novel Human Monocyte Subsets and Evidence for Phenotypic Groups Defined by Interindividual Variations of Expression of Adhesion Molecules

Identification of Novel Human Monocyte Subsets and Evidence for Phenotypic Groups Defined by Interindividual Variations of Expression of Adhesion Molecules

Association of serum lipids and severity of epithelial ovarian cancer: an observational cohort study of 349 Chinese patients

Ligation of Na, K ATPase β3 subunit on monocytes by a specific monoclonal antibody mediates T cell hypofunction

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