1977
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Abstract: The chemotactic activity of normal and diluted human serum and plasma for human monocytes and neutrophils was investigated. Undiluted, neither serum nor plasma attract monocytes, but this is attributable to an inhibitor masking chemotactic activity. When diluted 3- to 100-fold, appreciable activity for monocyte is detected. In contrast, chemotactic activity for neutrophils is already demonstrable in undiluted normal serum. Chemotactic activities are heat-labile, behave as pseudoglobulins, do not pass through a… Show more

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Cited by 13 publications
(10 citation statements)
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References 18 publications
(38 reference statements)
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“…Nevertheless, serum-stimulated chemokinesis comparable with the 25 % observed in response to serum would be expected since this does not depend upon the activation of complement (Jungi, 1977). However, no migration whatsoever was observed.…”
Section: Effects Of Altering Lntracellular and Extracellular Ca 2÷ Onsupporting
confidence: 52%
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“…Nevertheless, serum-stimulated chemokinesis comparable with the 25 % observed in response to serum would be expected since this does not depend upon the activation of complement (Jungi, 1977). However, no migration whatsoever was observed.…”
Section: Effects Of Altering Lntracellular and Extracellular Ca 2÷ Onsupporting
confidence: 52%
“…Chemokinesis stimulated by serum alone can be studied in these chemotaxis chambers by simply omitting the step of coating the erythrocytes with antibody. Unopsonized sheep erythrocytes do not activate the human complement cascade to any significant extent (Whaley, 1987), and the neutrophils migrate randomly on the surface in response to the chemoattractant present in human serum in which the complement cascade has not been activated (Jungi, 1977).…”
Section: Resultsmentioning
confidence: 99%
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“…After standing 40 min at room temperature, the red cells had sedimented and the leukocyte-rich plasma was aspirated and collected by centrifugation at 180 g for 10 min [Jungi, 1977]. Remaining erythrocytes were lysed by a 3-min exposure to buffered ammonium chloride after which the residual cells were washed, resuspended in medium 199 containing 1% BSA, and adjusted to 2 x l0 6 PMNs per milliliter.…”
Section: Pm N Chemotaxismentioning
confidence: 99%
“…The process of heat inactivation routinely employed in preparing PHS may itself generate substances which stimulate PMN locomotion (29). It is also known that fresh human serum contains chemotactic and chemokinetic factors (30,31), and further, that exposure of untreated serum to agarose can activate the comple-, ment system (8). Although the latter properties of serum are reportedly heat sensitive, there may be sufficient variation in lability among some donor sera to leave detectable residual activity.…”
Section: Duncan's Multiple Range Test (22)mentioning
confidence: 99%