Monoclonal Antibody-Based Enzyme Immunoassay for Detection of Ricin

Shyu, Huey-Fen; Chiao, Der-Jiang; Liu, Hwan-Wun; Tang, Shiao-Shek

doi:10.1089/15368590252917665

Cited by 68 publications

(37 citation statements)

References 12 publications

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“…Of these, ricin immuno-based detection methods, such as enzyme-linked immunosorbent assays (ELISA) and handheld lateral flow immunochromatographic devices (LFID) (Dayan-Kenigsberg et al, 2008;Fulton and Thompson, 2007), are heavily represented in the literature and have advantages in specificity, simplicity, and analysis time. Surprisingly, some of the earliest ELISA methods developed using colorimetric and chemi-luminescence detection are still competitive in terms of detection limits (Koja et al, 1980;Poli et al, 1994;Shyu et al, 2002a). The use of fluorescence-based fiber optics (Narang et al, 1997), colloidal gold particles (Shyu et al, 2002b), and electrochemiluminescence (Garber and O'Brien, 2008), has significantly improved assay times without sacrificing the sensitivity associated with the classical ELISAs.…”

Section: Methods That Cannot Identify Biologically Active Ricinmentioning

confidence: 99%

Ricin detection: Tracking active toxin

Bozza

Tolleson

Rosado

et al. 2015

Biotechnology Advances

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Ricin is a plant toxin with high bioterrorism potential due to its natural abundance and potency in inducing cell death. Early detection of the active toxin is essential for developing appropriate countermeasures. Here we review concepts for designing ricin detection methods, including mechanism of action of the toxin, advantages and disadvantages of current detection assays, and perspectives on the future development of rapid and reliable methods for detecting ricin in environmental samples.

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Section: Methods That Cannot Identify Biologically Active Ricinmentioning

confidence: 99%

Ricin detection: Tracking active toxin

Bozza

Tolleson

Rosado

et al. 2015

Biotechnology Advances

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show abstract

“…The CWAs and volatile degradation products are analyzed by gas chromatographymass spectrometry (GC-MS), 10,70) and polar decomposition products are structurally analyzed by liquid chromatography (LC)-MS. 71) The low MW biological toxins and the degradation products are analyzed by LC-MS, 72) and the toxin activity is measured by bioassay 73) or lateral flow immunoassay. 61) The proteinous toxins are analyzed quantitatively by spectroscopy (protein assay, organic materials index) and enzyme-linked immunoabsorbent assay, 74) and qualitatively by capillary electrophoresis, 75) gel electrophoresis, 76) surface plasmon resonance (SPR) spectrometry, 77) LC-MS 78) and matrixassisted laser ionization MS. 75,76) The cytotoxic activity is measured by cell culture assay 79) and bioassay, 80) and the enzymatic activity is measured by colorimetric assay. 81,82) The viruses are measured by plaque forming test, and analyzed structurally by electron microscopy.…”

Section: Methods For Estimating Decontamination Technologymentioning

confidence: 99%

Research and Development of On-site Decontamination System for Biological and Chemical Warfare Agents

Seto

2011

JOURNAL OF HEALTH SCIENCE

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Biological and chemical warfare agents (BCWAs) are diverse in nature including synthetic low molecular weight chemical warfare agents (gaseous choking and blood agents), volatile nerve gases and blister agents, nonvolatile vomit agents and lachrymators, biological toxins (polar low molecular weight toxins and proteinous toxins), and microorganisms (viruses, rickettsia, chlamydia and bacteria). In the consequence management against chemical and biological warfare terrorism, speedy decontamination of casualties, goods, items and equipments, buildings and field is required for the minimization of the terrorism damage. At present, washing casualties and contaminated materials with large volumes of water is the basic way, and hypochlorite solution is mainly used to decompose BCWAs. However, it still remains unsolved how to dispose the waste water contaminated with BCWAs, and decontaminating reagents have serious problems of high toxicity to human, despoiling nature to environment, long finishing time and non-durability. In addition, the present decontamination technologies are not effective, nonspecifically affecting the surrounding non-target materials. Therefore, it is the urgent matter to build up usable decontamination system surpassing the present system. The author introduces the joint project of research and development of the novel decontamination system against BCWAs, in the purpose of realizing non-dangerous, rapid, specific, effective and economical on-site decontamination. The project consists of establishment of the evaluation methods for decontamination, and verification of the present technologies and utilization of bacterial organophosphorus hydrolase, development of specific adsorptive elimination technologies using molecular recognition devices, and development of deactivation technologies using photocatalysis.

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“…Original methods employed animal experiments for measuring toxicity as well as quantitation of the biologically active material [5]. As immunological techniques became available, antibody microassays were successfully used to detect and quantify specific ricin proteins in complex mixtures [6,7]. Many antibody microassays utilized ELISA methods in either the indirect format or the sandwich format.…”

Section: Introductionmentioning

confidence: 99%

The DNA aptamers that specifically recognize ricin toxin are selected by twoin vitro selection methods

Tang¹,

Xie²,

Shao³

et al. 2006

Electrophoresis

114

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Aptamers which specifically recognize cytotoxin ricin were successfully selected using the two different in vitro selection methods. One selection method was used to isolate aptamers by affinity chromatography. Another selection method, named CE-SELEX, was carried out using CE as a separation approach. The high separation efficiency of CE evidently improved the rate of enrichment and obviously shortened the selection rounds, with near 87.2% binding just after the fourth round of selection. The aptamers A3, C1, and C5, derived from the two selection methods, were found to possess high affinity and specificity for ricin with the Kd values in the low nanomolar range, and did not recognize abrin toxin similar to ricin in the structures and properties, or BSA. Among the aptamers selected, A3 isolated by affinity chromatography shared extensive sequence similarity with C1 and C5 derived from CE-SELEX. They differed by only one base from each other. Their stable secondary structures predicted also had very similar structure motifs, and all folded a long and internal loop-embedded loop stem structure by base pairing. The ELISA and dot-blot analysis also proved that the selected DNA aptamers had the high specificity to ricin toxin.

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Monoclonal Antibody-Based Enzyme Immunoassay for Detection of Ricin

Cited by 68 publications

References 12 publications

Ricin detection: Tracking active toxin

Ricin detection: Tracking active toxin

Research and Development of On-site Decontamination System for Biological and Chemical Warfare Agents

The DNA aptamers that specifically recognize ricin toxin are selected by twoin vitro selection methods

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