Monoclonal antibodies to rhodopsin and other proteins of rod outer segments

Expression of CNTF Receptor-α in Chick Violet-Sensitive Cones with Unique Morphologic Properties

“…12 To determine the identity of CNTFR␣-positive photoreceptors, retinas were double labeled using the anti-CNTFR␣ antibody and the monoclonal antibody rho-4D2 raised against bovine rhodopsin, which has been shown to recognize rod OS in the chick retina. 8,26 The OS labeled by the two antibodies were very similar in shape, but the density of rho-4D2-positive OS was substantially higher ( Figs. 2A, 2B) and there was no colocalization of the two signals ( Fig.…”

Expression of CNTF Receptor-α in Chick Violet-Sensitive Cones with Unique Morphologic Properties

“…12 To determine the identity of CNTFR␣-positive photoreceptors, retinas were double labeled using the anti-CNTFR␣ antibody and the monoclonal antibody rho-4D2 raised against bovine rhodopsin, which has been shown to recognize rod OS in the chick retina. 8,26 The OS labeled by the two antibodies were very similar in shape, but the density of rho-4D2-positive OS was substantially higher ( Figs. 2A, 2B) and there was no colocalization of the two signals ( Fig.…”

Highly Differentiated Human Fetal RPE Cultures Are Resistant to the Accumulation and Toxicity of Lipofuscin-Like Material

“…In addition, UAM-laden hfRPE were less efficient at fully digesting a single regular OS bolus. While initial OS digestion rates were similar, UAM-laden hfRPE demonstrated longer retention of partially degraded rhodopsin fragments, as assessed by anti-rhodopsin antibodies targeting both rhodopsin C-terminus (1D4)23 and N-terminus (MCA-B630)22 (Fig. 5A).…”

Mislocalized Rhodopsin Does Not Require Activation to Cause Retinal Degeneration and Neurite Outgrowth inXenopus laevis

“…We identified an antibody, 2B2, generated against bovine rhodopsin N-terminal region that did not cross-react with X. laevis rhodopsin (Hicks and Molday, 1986). The region corresponding to the mapped epitope (Molday, 1988) differs by only one amino acid between bovine and X. laevis rhodopsin (F13 vs M13). By introducing the mutation M13F, we conferred 2B2 reactivity to X. laevis rhodopsin.…”

Expression of CNTF Receptor-α in Chick Violet-Sensitive Cones with Unique Morphologic Properties

“…12 To determine the identity of CNTFR␣-positive photoreceptors, retinas were double labeled using the anti-CNTFR␣ antibody and the monoclonal antibody rho-4D2 raised against bovine rhodopsin, which has been shown to recognize rod OS in the chick retina. 8,26 The OS labeled by the two antibodies were very similar in shape, but the density of rho-4D2-positive OS was substantially higher ( Figs. 2A, 2B) and there was no colocalization of the two signals ( Fig.…”

Highly Differentiated Human Fetal RPE Cultures Are Resistant to the Accumulation and Toxicity of Lipofuscin-Like Material

“…In addition, UAM-laden hfRPE were less efficient at fully digesting a single regular OS bolus. While initial OS digestion rates were similar, UAM-laden hfRPE demonstrated longer retention of partially degraded rhodopsin fragments, as assessed by anti-rhodopsin antibodies targeting both rhodopsin C-terminus (1D4)23 and N-terminus (MCA-B630)22 (Fig. 5A).…”

Mislocalized Rhodopsin Does Not Require Activation to Cause Retinal Degeneration and Neurite Outgrowth inXenopus laevis

“…We identified an antibody, 2B2, generated against bovine rhodopsin N-terminal region that did not cross-react with X. laevis rhodopsin (Hicks and Molday, 1986). The region corresponding to the mapped epitope (Molday, 1988) differs by only one amino acid between bovine and X. laevis rhodopsin (F13 vs M13). By introducing the mutation M13F, we conferred 2B2 reactivity to X. laevis rhodopsin.…”

Expression of CNTF Receptor-α in Chick Violet-Sensitive Cones with Unique Morphologic Properties

“…12 To determine the identity of CNTFR␣-positive photoreceptors, retinas were double labeled using the anti-CNTFR␣ antibody and the monoclonal antibody rho-4D2 raised against bovine rhodopsin, which has been shown to recognize rod OS in the chick retina. 8,26 The OS labeled by the two antibodies were very similar in shape, but the density of rho-4D2-positive OS was substantially higher ( Figs. 2A, 2B) and there was no colocalization of the two signals ( Fig.…”

Highly Differentiated Human Fetal RPE Cultures Are Resistant to the Accumulation and Toxicity of Lipofuscin-Like Material

“…In addition, UAM-laden hfRPE were less efficient at fully digesting a single regular OS bolus. While initial OS digestion rates were similar, UAM-laden hfRPE demonstrated longer retention of partially degraded rhodopsin fragments, as assessed by anti-rhodopsin antibodies targeting both rhodopsin C-terminus (1D4)23 and N-terminus (MCA-B630)22 (Fig. 5A).…”

Mislocalized Rhodopsin Does Not Require Activation to Cause Retinal Degeneration and Neurite Outgrowth inXenopus laevis

“…We identified an antibody, 2B2, generated against bovine rhodopsin N-terminal region that did not cross-react with X. laevis rhodopsin (Hicks and Molday, 1986). The region corresponding to the mapped epitope (Molday, 1988) differs by only one amino acid between bovine and X. laevis rhodopsin (F13 vs M13). By introducing the mutation M13F, we conferred 2B2 reactivity to X. laevis rhodopsin.…”