1982
DOI: 10.1073/pnas.79.24.7929
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Abstract: A panel of 148 monoclonal antibodies directed against Drosophila neural antigens has been prepared by using mice immunized with homogenates ofDrosophila tissue. Antibodies were screened immunohistochemically on cryostat sections of fly heads. A large diversity of staining patterns was observed. Some antigens were broadly distributed among tissues; others were highly specific to nerve fibers, neuropil, muscle, the tracheal system, cell nuclei, photoreceptors, or other structures. The antigens for many of the an… Show more

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Cited by 465 publications
(270 citation statements)
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“…Nervous system development was examined by staining with mAb 22C10, a monoclonal antibody that recognizes the microtubule-associated protein futsch (5). mAb 22C10 stains cell bodies and axons of all neurons in the peripheral nervous system (PNS) and a subset of neurons in the ventral nerve cord (VNC) of the central nervous system (CNS) (6). Therefore, RNAiinduced mutant phenotypes, such as disruption of the nervous system, collapse of axon tracts, fasciculation defects, or loss or gain of neurons, often can be distinguished from wild-type embryos.…”
Section: Resultsmentioning
confidence: 99%
“…Nervous system development was examined by staining with mAb 22C10, a monoclonal antibody that recognizes the microtubule-associated protein futsch (5). mAb 22C10 stains cell bodies and axons of all neurons in the peripheral nervous system (PNS) and a subset of neurons in the ventral nerve cord (VNC) of the central nervous system (CNS) (6). Therefore, RNAiinduced mutant phenotypes, such as disruption of the nervous system, collapse of axon tracts, fasciculation defects, or loss or gain of neurons, often can be distinguished from wild-type embryos.…”
Section: Resultsmentioning
confidence: 99%
“…Adult CNSs were dissected as described previously (Malpel et al, 2002), except for anti-CRY labeling, in which dissections were performed under red light, and fixation was done in the dark. Dilutions for the primary antibodies were as follows: mouse antichaoptin mAb24B10 (Fujita et al, 1982) and anti-GLASS mAb9B2.1 (Ellis et al, 1993) monoclonal antibodies, 1/100; rabbit anti-PER serum (Stanewsky et al, 1997) adsorbed against adult per 0 acetone powder, 1/15000; and guinea-pig anti-PDF antiserum (Neosystem Laboratoire, Strasbourg, France), 1/200. The rabbit anti-CRY antiserum was also raised by Neosystem Laboratoire against a peptide in the N-terminal half of Drosophila CRY (amino acids 176 -193).…”
Section: Methodsmentioning
confidence: 99%
“…Analysis of SNARE complex levels was performed using a monoclonal antibody (mAb) to syntaxin1A (Fujita et al, 1982) as previously described (Tolar and Pallanck, 1998). To minimize experimental variability arising from Western blot analysis, all experimental samples analyzed were loaded adjacent to samples prepared from Canton-S flies (wild-type).…”
Section: Methodsmentioning
confidence: 99%