Mono- and dicarboxylic polypyridyl-Ru complexes as potential cell DNA dyes and transfection agents

Musatkina, E. A.; Amouri, Hani; Lamoureux, M.; Chepurnykh, T. V.; Cordier, C.

doi:10.1016/j.jinorgbio.2007.03.011

Cited by 36 publications

(20 citation statements)

References 18 publications

(25 reference statements)

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“…The fact that these complexes have a strong capacity to condense DNA, which can be fine-tuned by small structural alterations, also makes them potential candidates for gene delivery. An interest in ruthenium(II) complexes as gene delivery vectors has recently emerged, and successful transfection using mononuclear ruthenium complexes has indeed been reported [38, 39]. In light of these findings, it would be of great interest to investigate the potential of our complexes, displaying great DNA-condensing capability combined with efficient internalization into cells, to function as DNA carriers.…”

Section: Discussionmentioning

confidence: 99%

Effects of chirality on the intracellular localization of binuclear ruthenium(II) polypyridyl complexes

et al. 2012

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Interest in binuclear ruthenium(II) polypyridyl complexes as luminescent cellular imaging agents and for biomedical applications is increasing rapidly. We have investigated the cellular localization, uptake, and biomolecular interactions of the pure enantiomers of two structural isomers of [μ-bipb(phen)4Ru2]4+ (bipb is bis(imidazo[4,5-f]-1,10-phenanthrolin-2-yl)benzene and phen is 1,10-phenanthroline) using confocal laser scanning microscopy, emission spectroscopy, and linear dichroism. Both complexes display distinct enantiomeric differences in the staining pattern of fixed cells, which are concluded to arise from chiral discrimination in the binding to intracellular components. Uptake of complexes in live cells is efficient and nontoxic at 5 μM, and occurs through an energy-dependent mechanism. No differences in uptake are observed between the structural isomers or the enantiomers, suggesting that the interactions triggering uptake are rather insensitive to structural variations. Altogether, these findings show that the complexes investigated are promising for future applications as cellular imaging probes. In addition, linear dichroism shows that the complexes exhibit DNA-condensing properties, making them interesting as potential gene delivery vectors.Electronic supplementary materialThe online version of this article (doi:10.1007/s00775-012-0877-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Effects of chirality on the intracellular localization of binuclear ruthenium(II) polypyridyl complexes

et al. 2012

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“…Several kinds of electrodes such as Pt, glassy carbon, TiO 2 and polypyrrole have been used for the oxidation and determination of BPA [34][35][36][37][38]. Based on the rich redox activities and well-known luminescence properties of polypyridyl ruthenium(II) complexes [39,40], they have been electrochemically used as novel probes of DNA [41][42][43], guanine [43][44][45] and hypoxanthine [46,47]. However, to our knowledge, there is no report on the electrochemical oxidation and detection of BPA mediated by the ruthenium(II) complexes.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical detection of bisphenol A mediated by [Ru(bpy)3]2+ on an ITO electrode

et al. 2010

Journal of Hazardous Materials

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“…Metal-ligand complexes of ruthenium, osmium and rhenium have been used as fluorescent labels in fluorescence polarisation immunoassays [5][6][7] and also in the detection of DNA through the hybridisation of an oligonucleotide probe that was labelled with a transition-metal complex and the target DNA molecule. [8][9][10] The complex [Ru II A C H T U N G T R E N N U N G (bpy) 2 A C H T U N G T R E N N U N G (deabpy)] 2 + (deabpy = 4,4-diethylaminomethyl-2,2-bipyridine) changes its emission spectrum depending on the concentration of H + in aqueous solutions and has been used as a fluorescent pH sensor. [11] A [Ru-A C H T U N G T R E N N U N G (bpy) 3 ] 2 + fragment covalently attached to a calix [4]arene receptor varied its luminescence in response to cations such as barium(II).…”

Section: Introductionmentioning

confidence: 99%

Use of an Osmium Complex as a Universal Luminescent Probe for Enzymatic Reactions

Virel

Sanchez-Lopez

Saá

et al. 2009

Chemistry A European J

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The water-soluble bis(bipyridine)chloro(4-picolinic acid) osmium complex, [Os(III)(bpy)2Cl(PyCOOH)]2+ (bpy=2,2'-bipyridine, Py=pyridine), is fluorescent in aqueous solution, whereas the reduced form of the complex, [Os(II)(bpy)2Cl(PyCOOH)]+, shows no significant fluorescence under the same conditions. Such reversible redox control of the fluorescence of the complex can be easily adapted to follow any enzymatic reaction to yield oxidising or reducing products that are capable of interacting with [Os(III)(bpy)2Cl(PyCOOH)]2+ or [Os(II)(bpy)2Cl(PyCOOH)]+. Based on the redox reaction between products of biocatalytic reactions and the osmium complex, we have designed a simple bioanalytical assay for the detection of nerve gases, alpha-ketoglutarate, hydrogen peroxide and glucose.

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Mono- and dicarboxylic polypyridyl-Ru complexes as potential cell DNA dyes and transfection agents

Cited by 36 publications

References 18 publications

Effects of chirality on the intracellular localization of binuclear ruthenium(II) polypyridyl complexes

Effects of chirality on the intracellular localization of binuclear ruthenium(II) polypyridyl complexes

Electrochemical detection of bisphenol A mediated by [Ru(bpy)3]2+ on an ITO electrode

Use of an Osmium Complex as a Universal Luminescent Probe for Enzymatic Reactions

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