Monkey Interleukin 2: Optimal Conditions for Production and Partial Characterization

Abstract

Cynomolgus monkey spleen cells and peripheral blood lymphocytes were tested for their ability to produce interleukin 2 (IL2) upon stimulation with phytomitogens. By assessing proliferation of murine IL 2-dependent cells, maximal release of IL 2 activity was found in the supernatants of nonadherent cells cultured for 48 h, after stimulation with 25 μg/ml concanavalin A. Addition of phorbol myristate acetate to the cultures resulted in enhancement of IL 2 production, but there was no significant promotion when indomethacin or human lymphoblastoid cell lines were added to the cultures. Adherent macrophages appeared to inhibit rather than enhance IL 2 production in this system. Monkey IL 2 exhibited a molecular weight in the range of 15,000–18,000 daltons in Sephadex G-100 gel chromatography.

Follow-up of stable chromosomal aberrations in gamma-ray irradiated non-human primates

“…Translocation frequency did not vary significantly according to a variety of culture conditions that we tested: the presence or absence of IL2, whether FCS accounted for 10% or 20% of the medium, and whether the culture lasted two or three days. These results are consistent with previously published results (Small et al 1985, Tatsumi et al 1986.…”