Monitoring the excited state of a fluorophore in a microscope by stimulated emission

Lens-based fluorescence nanoscopy

“…1990), and, in a pulsed STED scheme, it is well known that photons should be detected after the STED pulse has left (Schrader et al . 1995; Westphal & Hell, 2005), as shown in a recent experiment using time-correlated single photon counting (Auksorius et al . 2008).…”

Lens-based fluorescence nanoscopy

“…1990), and, in a pulsed STED scheme, it is well known that photons should be detected after the STED pulse has left (Schrader et al . 1995; Westphal & Hell, 2005), as shown in a recent experiment using time-correlated single photon counting (Auksorius et al . 2008).…”

Fluorescence Lifetime Imaging Microscopy with Subdiffraction-Limited Resolution

“…In pulsed STED microscopy, the depletion was maximal at a delay of approximately the temporal duration of the STED pulse. 19) In our experiment, the pulse width of STED was stretched to approximately 300 ps. Therefore, the initial part of the fluorescence decay curve was strongly affected by the STED beam, resulting in the forced rapid drop that can be seen in the curve.…”

Photon counting based pump-probe technique for quantitative characterization of fluorescence in a lock-in free detection manner

“…Figure 1(a) shows the two-level energy diagram involved in typical fluorophores excitation and emission processes [18][19][20]. The symbols S 0 and S 1 represent the relaxed ground state and first singlet electronic excited state.…”

Lens-based fluorescence nanoscopy

“…1990), and, in a pulsed STED scheme, it is well known that photons should be detected after the STED pulse has left (Schrader et al . 1995; Westphal & Hell, 2005), as shown in a recent experiment using time-correlated single photon counting (Auksorius et al . 2008).…”

Fluorescence Lifetime Imaging Microscopy with Subdiffraction-Limited Resolution

“…In pulsed STED microscopy, the depletion was maximal at a delay of approximately the temporal duration of the STED pulse. 19) In our experiment, the pulse width of STED was stretched to approximately 300 ps. Therefore, the initial part of the fluorescence decay curve was strongly affected by the STED beam, resulting in the forced rapid drop that can be seen in the curve.…”

Photon counting based pump-probe technique for quantitative characterization of fluorescence in a lock-in free detection manner

“…Figure 1(a) shows the two-level energy diagram involved in typical fluorophores excitation and emission processes [18][19][20]. The symbols S 0 and S 1 represent the relaxed ground state and first singlet electronic excited state.…”

Lens-based fluorescence nanoscopy

“…1990), and, in a pulsed STED scheme, it is well known that photons should be detected after the STED pulse has left (Schrader et al . 1995; Westphal & Hell, 2005), as shown in a recent experiment using time-correlated single photon counting (Auksorius et al . 2008).…”

Fluorescence Lifetime Imaging Microscopy with Subdiffraction-Limited Resolution

“…In pulsed STED microscopy, the depletion was maximal at a delay of approximately the temporal duration of the STED pulse. 19) In our experiment, the pulse width of STED was stretched to approximately 300 ps. Therefore, the initial part of the fluorescence decay curve was strongly affected by the STED beam, resulting in the forced rapid drop that can be seen in the curve.…”

Photon counting based pump-probe technique for quantitative characterization of fluorescence in a lock-in free detection manner

“…Figure 1(a) shows the two-level energy diagram involved in typical fluorophores excitation and emission processes [18][19][20]. The symbols S 0 and S 1 represent the relaxed ground state and first singlet electronic excited state.…”