Monitoring the colonization of sugarcane and rice plants by the endophytic diazotrophic bacterium Gluconacetobacter diazotrophicus marked with gfp and gusA reporter genes

Rouws, Luc Felicianus Marie; Meneses, Carlos; Guedes, Helma V.; Vidal, Márcia Soares; Baldani, José Ivo; Schwab, Stefan

doi:10.1111/j.1472-765x.2010.02899.x

Cited by 50 publications

(38 citation statements)

References 18 publications

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“…Later, microscopy studies have also been extended to seed plants such as rice (Rouws et al 2010), which was also shown to be endophytically colonized. In fact, hydroponically grown rice seedlings have proven to be a valuable model system for G. diazotrophicus-plant interactions, allowing rapid assays and presenting low autofluorescence, which facilitates microscopy studies (Rouws et al 2010). However, the micropropagation technique is highly time consuming and laborious.…”

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confidence: 99%

Exopolysaccharide Production Is Required for Biofilm Formation and Plant Colonization by the Nitrogen-Fixing Endophyte Gluconacetobacter diazotrophicus

Meneses¹,

Rouws²,

Simões‐Araújo³

et al. 2011

MPMI

183

104

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The genome of the endophytic diazotrophic bacterial species Gluconacetobacter diazotrophicus PAL5 (PAL5) revealed the presence of a gum gene cluster. In this study, the gumD gene homologue, which is predicted to be responsible for the first step in exopolysaccharide (EPS) production, was insertionally inactivated and the resultant mutant (MGD) was functionally studied. The mutant MGD presented normal growth and nitrogen (N(2)) fixation levels but did not produce EPS when grown on different carbon sources. MGD presented altered colony morphology on soft agar plates (0.3% agar) and was defective in biofilm formation on glass wool. Most interestingly, MGD was defective in rice root surface attachment and in root surface and endophytic colonization. Genetic complementation reverted all mutant phenotypes. Also, the addition of EPS purified from culture supernatants of the wild-type strain PAL5 to the mutant MGD was effective in partially restoring wild-type biofilm formation and plant colonization. These data provide strong evidence that the PAL5 gumD gene is involved in EPS biosynthesis and that EPS biosynthesis is required for biofilm formation and plant colonization. To our knowledge, this is the first report of a role of EPS in the endophytic colonization of graminaceous plants by a nitrogen-fixing bacterium.

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mentioning

confidence: 99%

Exopolysaccharide Production Is Required for Biofilm Formation and Plant Colonization by the Nitrogen-Fixing Endophyte Gluconacetobacter diazotrophicus

Meneses¹,

Rouws²,

Simões‐Araújo³

et al. 2011

MPMI

183

104

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“…Error bars represent the standard deviation. Significant differences between treatments are represented by asterisk (ANOVA,p<0.05) interaction between plants and bacteria isolated from grasses, including G. diazotrophicus (Rouws et al 2010;Meneses et al 2011;Alquéres et al 2013). In t h e c u r r e n t s t u d y, w e d e m o n s t r a t e d t h a t G. diazotrophicus endophytically colonizes A. thaliana roots and promotes plant growth.…”

Section: Discussionmentioning

confidence: 99%

Endophytic colonization of Arabidopsis thaliana by Gluconacetobacter diazotrophicus and its effect on plant growth promotion, plant physiology, and activation of plant defense

Souza

Oliveira

et al. 2015

Plant Soil

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Background and aims Gluconacetobacter diazotrophicus is a plant growth-promoting bacteria (PGPB) that colonizes several plant species. Here, we studied the internal colonization of Arabidopsis thaliana tissues by G. diazotrophicus and analyzed its effects on physiology, growth, and activation of plant immune system during such association.Methods A. thaliana seedlings were inoculated with G. diazotrophicus and grown in substrate for 50 days. Effects on plant growth were estimated by quantifying number of leaves, leaf area, and fresh and dry weight. Endophytic bacterial population was determined by colony-forming unit (CFU), and its location in plant tissues was assayed by epifluorescence microscopy of red fluorescent protein-labeled bacterium. Whole canopy gas exchange (photosynthesis and transpiration) was determined using a portable photosynthesis system. Results G. diazotrophicus efficiently promoted A. thaliana plant growth at 50 days after inoculation. Inoculated plants showed higher whole canopy photosynthesis, lower whole plant transpiration, and increased water-use efficiency. The bacterium colonized preferentially root xylem. The inoculation of plants defective in systemic acquired resistance (SAR)-associated defense revealed that plant immune system plays an important role during the early association stages. Conclusions G. diazotrophicus endophytically colonizes A. thaliana roots, promotes plant growth, and increases whole canopy photosynthesis. Our results indicate that A. thaliana is useful for molecular studies of the mechanisms involved in the interaction between plants and PGPB, especially those involving G. diazotrophicus.

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“…B. pseudomallei has been found in soil at levels of up to 10 5 CFU/g (6), so growth retardation in natural soils may not be as obvious or may allow persistent interactions between the plant and the microbe. The brevity of this experiment (7 days) and growth in Hoagland agar, although it is also commonly used (26,33,34), are not adequate for full rice growth and may not mimic growth in soil. It would be interesting to study B. pseudomallei-O.…”

Section: Discussionmentioning

confidence: 99%

Effects of Colonization of the Roots of Domestic Rice (Oryza sativa L. cv. Amaroo) by Burkholderia pseudomallei

Prasertsincharoen

Constantinoiu

Gardiner

et al. 2015

Appl Environ Microbiol

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bBurkholderia pseudomallei is a saprophytic bacterium that causes melioidosis and is often isolated from rice fields in Southeast Asia, where the infection incidence is high among rice field workers. The aim of this study was to investigate the relationship between this bacterium and rice through growth experiments where the effect of colonization of domestic rice (Oryza sativa L. cv Amaroo) roots by B. pseudomallei could be observed. When B. pseudomallei was exposed to surface-sterilized seeds, the growth of both the root and the aerosphere was retarded compared to that in controls. The organism was found to localize in the root hairs and endodermis of the plant. A biofilm formed around the root and root structures that were colonized. Growth experiments with a wild rice species (Oryza meridionalis) produced similar retardation of growth, while another domestic cultivar (O. sativa L. cv Koshihikari) did not show retarded growth. Here we report B. pseudomallei infection and inhibition of O. sativa L. cv Amaroo, which might provide insights into plant interactions with this important human pathogen. Melioidosis is caused by the aerobic Gram-negative bacillus Burkholderia pseudomallei, which is common in Northern Australia and Southeast Asia, where the mortality rate can be as high as 40% (1). B. pseudomallei is recognized as a soil-and waterborne pathogen and is commonly isolated from the soil of rice fields and other environmental sources where the organism is endemic (2-7). Exposure to the organism in rice paddies is a significant risk to rice farmers, particularly those who are also diabetic and are generally more predisposed to infection (8).The rhizosphere is a rich habitat for bacteria. Microbial interaction with plants can result in a variety of effects, ranging from pathogenesis to growth promotion (9), and plants may facilitate the persistence of the microorganism (10). Bacteria in the Burkholderia genus are well known to be plant endophytes, some with plant growth promotion capacity and some as pathogens (11). As B. pseudomallei has a broad host range (12-17) and has been shown to infect other species of plants (18,19), it may be possible that it will infect some cultivars of rice and a plant-microbe interaction may assist in the persistence of B. pseudomallei in the rice fields where it is commonly found. However, no studies have so far identified any growth promotion or pathogenic relationship between B. pseudomallei and rice. Lee et al. (19) found no effects on a Japanese rice cultivar (Oryza sativa cv. Nipponbare), while Kaestli et al. (18) found no effects on an Australian wild rice species (Oryza rufipogon). An examination of the interaction of B. pseudomallei with more cultivars and species of rice could provide more information about whether rice is, in general, resistant to B. pseudomallei or identify a potential host for an infection model. The development of a rice model of B. pseudomallei infection has the potential to assist in further plant-microbe interaction studies and the developmen...

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Monitoring the colonization of sugarcane and rice plants by the endophytic diazotrophic bacterium Gluconacetobacter diazotrophicus marked with gfp and gusA reporter genes

Cited by 50 publications

References 18 publications

Exopolysaccharide Production Is Required for Biofilm Formation and Plant Colonization by the Nitrogen-Fixing Endophyte Gluconacetobacter diazotrophicus

Exopolysaccharide Production Is Required for Biofilm Formation and Plant Colonization by the Nitrogen-Fixing Endophyte Gluconacetobacter diazotrophicus

Endophytic colonization of Arabidopsis thaliana by Gluconacetobacter diazotrophicus and its effect on plant growth promotion, plant physiology, and activation of plant defense

Effects of Colonization of the Roots of Domestic Rice (Oryza sativa L. cv. Amaroo) by Burkholderia pseudomallei

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