1996
DOI: 10.1021/bk-1996-0645.ch023
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Abstract: We discuss assay approaches for monitoring the sensitivity of Lepidoptera to Bacillus thuringiensis (Bt) insecticidal proteins and compare the relative sensitivity of larval feeding bioassays in which, respectively, mortality or growth inhibition were scored. Heliothis virescens (F.) and Helicoverpa zea (Boddie), major lepidopteran pests targeted for control by transgenic cotton, were used for assay comparison. Larval growth inhibition assays using sublethal CryIA(c) protein concentrations were considerably mo… Show more

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Cited by 50 publications
(60 citation statements)
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“…Stone and Sims (1993) found considerable interpopulation variation in B. thuringiensis susceptibility to Cry1Ac and formulated Dipel among U.S. populations of both corn earworm, Helicoverpa zea (Boddie), and tobacco budworm Heliothis virescens (16-and 4-fold, respectively). These data were reexamined by Sims et al (1996), who suggested that interpopulation variation in B. thuringiensis susceptibility may reßect nongenetic variation or sampling error, because the populations tested represented a small sample, taken at one point in time, of considerably larger multivoltine populations. Similar levels of variability in susceptibility to Cry1Ab and Cry1Ac were observed among European corn borer populations (Març on et al 1999) that included populations of different voltine ecotypes and pheromone strains and among geographically distinct populations of H. zea exposed to Cry1Ab (Siegfried et al 2000).…”
Section: Resultsmentioning
confidence: 99%
“…Stone and Sims (1993) found considerable interpopulation variation in B. thuringiensis susceptibility to Cry1Ac and formulated Dipel among U.S. populations of both corn earworm, Helicoverpa zea (Boddie), and tobacco budworm Heliothis virescens (16-and 4-fold, respectively). These data were reexamined by Sims et al (1996), who suggested that interpopulation variation in B. thuringiensis susceptibility may reßect nongenetic variation or sampling error, because the populations tested represented a small sample, taken at one point in time, of considerably larger multivoltine populations. Similar levels of variability in susceptibility to Cry1Ab and Cry1Ac were observed among European corn borer populations (Març on et al 1999) that included populations of different voltine ecotypes and pheromone strains and among geographically distinct populations of H. zea exposed to Cry1Ab (Siegfried et al 2000).…”
Section: Resultsmentioning
confidence: 99%
“…The ratio between the weight of treated and untreated larvae at day seven was used to calculate larval growth inhibition. The effective concentrations that produce a reduction in larval growth of 50 % (EC 50 ) and 99 % (EC 99 ) were calculated by adjusting the curve as proposed by Sims et al (1996).…”
Section: Methodsmentioning
confidence: 99%
“…The nonlinear logistic models used for the computation of EC50 (Sims et al 1996) and EC90 (adapted from Sims et al 1996) were, respectively: Where W0 is the expected control weight, concentration is the amount of Vip3Aa20 protein/cm 2 of diet, and B is the logistic function slope parameter (SIMS et al, 1996).…”
Section: Discussionmentioning
confidence: 99%
“…To estimate the diagnostic concentration, the concentration-mortality data of all populations were analyzed jointly, according to the method proposed by SIMS et al (1996). In the joint analysis, mortality data were fitted with a binomial model using the log-log complement connection function (gompit) in SAS 9.1 (PROC PROBIT, SAS INSTITUTE INC., 2004) to obtain LC99.…”
Section: Discussionmentioning
confidence: 99%
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