Monitoring ovarian cycles and pregnancy in brown brocket deer (Mazama gouazoubira) by measurement of fecal progesterone metabolites

Pereira, Ricardo José Garcia; Polegato, Bruna Furlan; Souza, Simone de; Negrão, João Alberto; Duarte, José Maurício Barbanti

doi:10.1016/j.theriogenology.2005.02.019

Cited by 45 publications

(42 citation statements)

References 21 publications

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“…All but 4 ewes had low plasma P 4 and faecal P 4 -met concentrations (< 1.50 nmol/l and < 250 nmol/g, respectively) at the time of AI, representing the range known as physiological in the follicular phase of cyclic small ruminants (Schwarzenberger et al, 1996;Pereira et al, 2006). None of these 4 individuals with elevated plasma P 4 (1.57 to 4.54 nmol/l) and faecal P 4 -met (268 to 424 nmol/g) contents conceived at that time.…”

Section: Resultsmentioning

confidence: 99%

Pregnancy stage and number of fetuses may influence maternal plasma leptin in ewes

Kulcsár¹,

Dankó²,

Magdy³

et al. 2006

Acta Veterinaria Hungarica

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Maternal plasma leptin is elevated in ewes during pregnancy. The authors studied whether there was any relation between maternal plasma leptin and insulin concentrations, the number of fetuses and the circulating and faecal levels of gestagens. At the end of the breeding season in January the ovarian activity of Prolific Merino ewes was induced/synchronised with gestagen + eCG treatment. Ewes were inseminated artificially (AI) by laparoscopy. Blood and faecal samples were collected before AI (day 0) and again 41, 81 and 101 days later. The plasma levels of leptin (pL), insulin and progesterone (pP 4 ), and the faecal P 4 metabolite (P 4 -met) content were determined. The day 0 level of pL was significantly higher in pregnant (n = 24) than in non-pregnant ewes (n = 32). By day 41 the pL of pregnant animals had doubled, it showed a further moderate increase on day 81, and decreased slightly thereafter. During pregnancy pP 4 and faecal P 4 -met rose continuously and were positively correlated at all stages. The mean levels of pL and pP 4 and the faecal content of P 4 -met were lower in ewes bearing single (n = 12) than in those with 2 (n = 6) or 3-5 fetuses (n = 6). Analysis of variance demonstrated significant differences according to the number of fetuses in the pL and pP 4 , but not in P4-met (p = 0.042, 0.044, and 0.051, respectively). Leptin showed positive correlation with insulin before the AI but not during pregnancy. On days 41 and 81 pL showed a slight positive correlation with P 4 and P 4 -met, which decreased slightly by day 101. This study shows that although leptinaemia is affected by the number of fetuses and the level of P 4 , pregnancy stage is a more important regulator than these additional factors.

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Section: Resultsmentioning

confidence: 99%

Pregnancy stage and number of fetuses may influence maternal plasma leptin in ewes

Kulcsár¹,

Dankó²,

Magdy³

et al. 2006

Acta Veterinaria Hungarica

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“…Los resultados obtenidos pueden deberse a varios factores, uno de ellos son las variaciones individuales en la excreción y metabolización de progesterona, las cuales pueden influir en los perfiles hormonales [14]; este hecho debe ser tomado en cuenta con cuidado, ya que se encontró gran variación en los resultados analizados de manera individual; si este hecho se debiera a las variaciones individuales, estas serían demasiado grandes para considerar que las progestinas fecales reflejan de manera indirecta la actividad lútea; sin embargo, estos metabolitos han sido considerados buenos indicadores de la actividad ovárica en felinos, primates, ungulados y especialmente en Mazama americana [15] y M. gouazoubira [16] y otras especies, tanto domésticas como de zoológico [14]. Brousset et al [17] reportan que las variables que afectan la concentración hormonal contenida en los excrementos son: constipación, diarrea, restricción de alimentos, cantidad de fibra en la dieta, tratamientos con antibióticos que afecten la microbiota intestinal y las características metabólicas de cada individuo, lo cual también ha sido reportado por Palme et al [18].…”

Section: Resultados Y Discusiónunclassified

Método no invasivo de diagnóstico de gestación en venadas Odocoileus virginianus. (Non-Invasive Method for Pregnancy Diagnosis in Odocoileus Virginianus.)

et al. 2015

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ResumenObjetivo: Valorar la eficiencia del diagnóstico de gestación mediante la estimación de niveles de progestinas fecales en venadas cola blanca O. virginianus en la época no reproductiva. Materiales y métodos: Se analizaron muestras sanguíneas y heces de nueve venadas cola blanca (VCB), semanalmente, durante dos meses en la época reproductiva. Adicionalmente, las VCB fueron expuestas a macho durante un mes durante la época reproductiva, y se colectaron heces dos veces fuera de esta, para medir los niveles de metabolitos de progestinas. Se determinó el valor de corte para diagnosticar gestación a partir de los niveles de metabolitos de progestinas fecales en fase luteal y folicular. La progesterona sanguínea fue medida por radioinmunoanálisis, y se correlacionó con los niveles de progestinas fecales. Los diagnósticos de gestación fueron relacionados con los partos. Resultados: La correlación total entre niveles de progestinas sanguíneas y metabolitos de progestinas fecales fue 0,37 (p<0,01). La variación de la correlación individual fue 0,104 hasta 0,99. El valor de corte para diagnosticar gestación fue 4000 ng/g en heces. La eficiencia del diagnóstico de gestación fue 77,78 %; el valor predictivo del diagnóstico positivo fue 75 %, y el negativo fue 80 %, respectivamente. Conclusiones: Es posible diagnosticar gestación fuera de la época de reproducción mediante la estimación de los niveles de metabolitos de progestinas fecales, en VCB en cautiverio, tomando como valor de corte 4000 ng/g de heces.

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“…Durante este período, o comportamento do macho foi observado quanto à ocorrência de agressividade, aproximação do operador e aceite de alimento diretamente da mão do operador. Com o intuito de facilitar a detecção e otimizar a utilização de todo o período de estro das fêmeas (duração aproximada de 2,2±0,3 dias a cada 24,7±1,2 dias em M. gouazoubira - Pereira et al 2006), optou-se por realizar a manipulação farmacológica do ciclo estral com o uso de 250μg de cloprostenol sódico (1,0mL Ciosin ® -Intervet/Schering Plough Coopers ® , Brasil), por via intramuscular (Zanetti et al 2010). O fármaco foi administrado a cada 11 dias até a detecção de quatro estros em cada uma das espécies.…”

Section: Desvio Lateral Do Pênis Para Uma Vagina Artiϐicial (Fedl)unclassified

Avaliação de dois métodos para condicionamento e coleta de sêmen em quatro espécies do gênero Mazama

2012

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Pesq. Vet. Bras. 32(7):658-662, julho 2012 658 RESUMO.-O desenvolvimento de técnicas não invasivas para a obtenção de sêmen de cervídeos facilita a criação de bancos genômicos, que são importantes instrumentos para a conservação ex situ e in situ. Este trabalho teve como objetivo criar uma metodologia não-invasiva de coleta de sêmen e comparar duas técnicas de coleta em quatro espécies do The development of noninvasive techniques for obtaining semen from deer facilitates the creation of genome banks, which are important tools for ex situ and in situ conservation. This study aimed to establish a noninvasive method of semen collection and compare two techniques of collection in four species of the genus Mazama: M. americana, M. gouazoubira, M. nana and M. nemorivaga. To achieve this, 6 males (M) and 2 females (F) of the species M. Americana, 3M and 2F of M. gouazoubira, 1M and 1F of M. nana and 2M and 1F of M. nemorivaga were used. For each technique tested, a period of habituation to animal handling was conducted; then, the two conditioning techniques and collection were evaluated. In the ϐirst, a female in estrus was used with lateral deviation of the penis to an artiϐicial vagina (FEDL), yielding collection from 50% of the males (100% from M. gouazoubira and 50% from M. americana), with no ejaculate from the remaining species. In the second technique, using a taxidermized dummy with urine from females in estrus (MUFE), no semen collection was possible. During all stages, male behavior was observed regarding the time of interest and approximation, the "Flehmen" response, the act of snifϐing or licking, exposure of the penis, erection, number of false mounts, attempts at copulation and the occurrence of aggression between the deer.

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Monitoring ovarian cycles and pregnancy in brown brocket deer (Mazama gouazoubira) by measurement of fecal progesterone metabolites

Cited by 45 publications

References 21 publications

Pregnancy stage and number of fetuses may influence maternal plasma leptin in ewes

Pregnancy stage and number of fetuses may influence maternal plasma leptin in ewes

Método no invasivo de diagnóstico de gestación en venadas Odocoileus virginianus. (Non-Invasive Method for Pregnancy Diagnosis in Odocoileus Virginianus.)

Avaliação de dois métodos para condicionamento e coleta de sêmen em quatro espécies do gênero Mazama

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