Monitoring of zonisamide in human breast milk and maternal plasma by solid‐phase extraction HPLC method

Abstract

An HPLC method was developed for the determination of zonisamide in human breast milk and plasma. Chromatographic separation was achieved using a Develosil CN analytical column with potassium dihydrogenphosphate buffer (pH 3.5 with milk, pH 2.5 with plasma)-acetonitrile as the mobile phase. Zonisamide and 1, 2-benzisoxazole-3-methansulfonamine acetate as internal standard were detected by ultraviolet absorbance at 240 nm. Zonisamide in breast milk and plasma was extracted by a rapid and simple procedure based on C(18) bonded-phase extraction. Determination of zonisamide in human breast milk and plasma was possible in the concentration range 0.05-20.0 microg/mL. The recoveries of zonisamide added to human breast milk and plasma were 79.5-85.0% and 86.3-93.1%, respectively, with coefficients of variation of less than 8.3% and 11.4% respectively. The mean concentrations of zonisamide in breast milk and plasma were 9.41 +/- 0.95 and 10.13 +/- 0.45 microg/mL, respectively. The average ratio between the breast milk concentration and plasma concentration (M/P ratio) was 0.93 +/- 0.09.

Desenvolvimiento y validación de métodos bioanalíticos por HPLC- DAD para la cuantificación de fenitoína e inhibidores de la Glicoproteína- P (elacridar y tariquidar) en plasma

“…Para la detección y cuantificación de fenitoína en muestras plasmáticas, se han desarrollado numerosos trabajos usando HPLC (2,6,7,9,11,12,(17)(18)(19). Sanches et al, Oliveira et al y Bhatti et al reportaron la detección de fenitoína a una longitud de onda de 210 nm, por obtenerse una mayor área de fenitoína (5,7,9), al igual que lo observado en el presente trabajo.…”

Desenvolvimiento y validación de métodos bioanalíticos por HPLC- DAD para la cuantificación de fenitoína e inhibidores de la Glicoproteína- P (elacridar y tariquidar) en plasma

“…Para la detección y cuantificación de fenitoína en muestras plasmáticas, se han desarrollado numerosos trabajos usando HPLC (2,6,7,9,11,12,(17)(18)(19). Sanches et al, Oliveira et al y Bhatti et al reportaron la detección de fenitoína a una longitud de onda de 210 nm, por obtenerse una mayor área de fenitoína (5,7,9), al igual que lo observado en el presente trabajo.…”

Development and Validation of HPLC Method for quantification of Zonisamide in Spiked Human Plasma

“…Although few methods have been reported for the estimation of ZON in biological fluids in previous literature. However, these methods have been reported for estimation of ZON in human breast milk 3 , which is not readily available. In another reported method, human serum matrix was used 4 , which is expensive.…”

New antiepileptic drugs and women

“…The calculated milk/maternal serum concentration ratios ranged from 0.7 to 0.9. 80,107,108 No adverse effects on the breastfed child have been reported. However, because of the comparably high relative infant dose (about 30%) reported in these cases, caution and close monitoring of the infant is advisable.…”

Desenvolvimiento y validación de métodos bioanalíticos por HPLC- DAD para la cuantificación de fenitoína e inhibidores de la Glicoproteína- P (elacridar y tariquidar) en plasma

“…Para la detección y cuantificación de fenitoína en muestras plasmáticas, se han desarrollado numerosos trabajos usando HPLC (2,6,7,9,11,12,(17)(18)(19). Sanches et al, Oliveira et al y Bhatti et al reportaron la detección de fenitoína a una longitud de onda de 210 nm, por obtenerse una mayor área de fenitoína (5,7,9), al igual que lo observado en el presente trabajo.…”

Development and Validation of HPLC Method for quantification of Zonisamide in Spiked Human Plasma

“…Although few methods have been reported for the estimation of ZON in biological fluids in previous literature. However, these methods have been reported for estimation of ZON in human breast milk 3 , which is not readily available. In another reported method, human serum matrix was used 4 , which is expensive.…”

New antiepileptic drugs and women

“…The calculated milk/maternal serum concentration ratios ranged from 0.7 to 0.9. 80,107,108 No adverse effects on the breastfed child have been reported. However, because of the comparably high relative infant dose (about 30%) reported in these cases, caution and close monitoring of the infant is advisable.…”

Desenvolvimiento y validación de métodos bioanalíticos por HPLC- DAD para la cuantificación de fenitoína e inhibidores de la Glicoproteína- P (elacridar y tariquidar) en plasma

“…Para la detección y cuantificación de fenitoína en muestras plasmáticas, se han desarrollado numerosos trabajos usando HPLC (2,6,7,9,11,12,(17)(18)(19). Sanches et al, Oliveira et al y Bhatti et al reportaron la detección de fenitoína a una longitud de onda de 210 nm, por obtenerse una mayor área de fenitoína (5,7,9), al igual que lo observado en el presente trabajo.…”

Development and Validation of HPLC Method for quantification of Zonisamide in Spiked Human Plasma

“…Although few methods have been reported for the estimation of ZON in biological fluids in previous literature. However, these methods have been reported for estimation of ZON in human breast milk 3 , which is not readily available. In another reported method, human serum matrix was used 4 , which is expensive.…”

New antiepileptic drugs and women

“…The calculated milk/maternal serum concentration ratios ranged from 0.7 to 0.9. 80,107,108 No adverse effects on the breastfed child have been reported. However, because of the comparably high relative infant dose (about 30%) reported in these cases, caution and close monitoring of the infant is advisable.…”