Monitoring of Lymphocyte Micronuclei among Newborns from Kragujevac in Central Serbia before and after Environmental Contamination

Milošević-Djordjević, Olivera; Grujičić, Darko; Arsenijevic, Slobodan; Marinković, Darko

doi:10.1620/tjem.205.1

Cited by 21 publications

(11 citation statements)

References 28 publications

(29 reference statements)

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“…In literature, all studies focusing on MN levels in umbilical cord blood reported low frequencies in BN T lymphocytes from newborns, consistent with our results of 1.53 per 1,000 cells (median) in the total population (Das and Karuppasamy 2009; Decordier et al 2007; Hando et al 1994; Levario-Carrillo et al 2005; Lope et al 2010, Milošević-Djordjević et al 2005, 2007; Nath et al 1995; Neri et al 2005; Pedersen et al 2009, 2010; Stankovic et al 2004; Wyatt et al 2007; Zalacain et al 2006). We observed a median MNMONO frequency of 0.44 per 1,000 cells and a median CBPI value of 1.58.…”

Section: Discussionsupporting

confidence: 92%

Maternal and Gestational Factors and Micronucleus Frequencies in Umbilical Blood: The NewGeneris Rhea Cohort in Crete

Loock

Fthenou

Decordier

et al. 2011

Environ Health Perspect

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Background: The use of cancer-related biomarkers in newborns has been very limited.Objective: We investigated the formation of micronuclei (MN) in full-term and preterm newborns and their mothers from the Rhea cohort (Crete), applying for the first time in cord blood a validated semiautomated analysis system, in both mono- and binucleated T lymphocytes.Methods: We assessed MN frequencies in peripheral blood samples from the mothers and in umbilical cord blood samples. We calculated MN in mononucleated (MNMONO) and binucleated (MNBN) T lymphocytes and the cytokinesis block proliferation index (CBPI) in 251 newborns (224 full term) and 223 mothers, including 182 mother–child pairs. Demographic and lifestyle characteristics were collected.Results: We observed significantly higher MNBN and CBPI levels in mothers than in newborns. In newborns, MNMONO and MNBN were correlated (r = 0.35, p < 0.001), and we found a moderate correlation between MNMONO in mothers and newborns (r = 0.26, p < 0.001). MNMONO frequencies in newborns were positively associated with the mother’s body mass index and inversely associated with gestational age and mother’s age, but we found no significant predictors of MNBN or CBPI in newborns.Conclusions: Although confirmation is needed by a larger study population, the results indicate the importance of taking into account both mono- and binucleated T lymphocytes for biomonitoring of newborns, because the first reflects damage expressed during in vivo cell division and accumulated in utero, and the latter includes additional damage expressed as MN during the in vitro culture step.

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Section: Discussionsupporting

confidence: 92%

Maternal and Gestational Factors and Micronucleus Frequencies in Umbilical Blood: The NewGeneris Rhea Cohort in Crete

Loock

Fthenou

Decordier

et al. 2011

Environ Health Perspect

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“…In the previous paper (Milošević-Djordjević et al 2005), we showed that environmental pollution, caused by the air strikes on "Zastava" complex in Kragujevac (the city in the central Serbia), in the spring of 1999, signifi cantly increased MN frequency in lymphocytes of newborns born 12 months after pollution. After 18 months of the pollution, environmental components had no noticeable effects on MN frequencies in newborns.…”

Section: Methodsmentioning

confidence: 90%

Micronuclei in Cord Blood Lymphocytes as a Biomarker of Transplacental Exposure to Environmental Pollutants

Milošević-Djordjević

Grujičić

Arsenijevic³

et al. 2007

Tohoku J. Exp. Med.

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“…Factors that may increase fetal susceptibility include higher rates of cell proliferation, the greater number of target cells at risk, lower immunologic competence, and decreased capacity to activate and detoxify carcinogens as well as to repair DNA (Anderson et al 2000;Perera et al 2004;Whyatt et al 2004). A great number of authors showed that the frequency of damages in genetic material of neonates and children, such as MN and chromosome aberrations, increased with the concentration of environmental agents (Bocskay et al 2005;Milošević-Djordjević et al 2005, 2007bNeri et al 2006). In addition to the environmental pollutants, the therapy prescribed to pregnant women as a uterine relaxant attracts great attention as well.…”

Section: Discussionmentioning

confidence: 99%

Treatment of Pregnant Women with a Betamimetic and Verapamil Increases the Micronuclei Frequency in Umbilical Cord Blood Lymphocytes

Grujičić

Milošević-Djordjević

Arsenijevic

et al. 2008

Tohoku J. Exp. Med.

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In prevention of preterm labor, betamimetics are used in gynecological practice mostly combined with antiarrhythmic verapamil because of their therapeutic cardiovascular side effects. The aim of this study was to investigate the influence of a betamimetic (ritodrine hydrochloride, fenoterol or hexoprenaline) and verapamil (administered to mothers) on the frequency of micronuclei (MN) in umbilical cord blood lymphocytes of neonates, using cytokinesis-block micronucleus test. The analyzed sample included 23 babies whose mothers received the therapy and 30 control babies whose mothers received no therapy. The average MN frequency was significantly higher in the neonates whose mothers received the therapy (8.13 ± 2.69 MN/1000 BN cells), in comparison with the baseline frequency in untreated controls (3.30 ± 2.63 MN/1000 BN cells), with probability p < 0.05. The highest MN frequency was found in neonates whose mothers received fenoterol and verapamil (2.8-fold i.e. 9.10 ± 3.00 MN/1000 BN cells), while ritodrine hydrochloride and hexoprenaline combined with verapamil induced 2.3-fold and 2.2-fold higher MN values than in controls (7.50 ± 3.33 MN/1000 BN cells and 7.29 ± 0.95 MN/1000 BN cells). Multiple linear regression analysis showed that MN frequency was affected only by the maternal therapeutic treatment, while the neonates' sex, maternal age, cigarette smoking, and therapeutic doses did not affect the MN frequency in umbilical lymphocytes of neonates. We conclude that the treatment of pregnant women with a betamimetic and verapamil significantly increases the MN frequency in umbilical cord blood lymphocytes of neonates, regardless to therapeutic doses.umbilical cord blood lymphocytes; micronuclei; betamimetic; verapamil.Tohoku

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Monitoring of Lymphocyte Micronuclei among Newborns from Kragujevac in Central Serbia before and after Environmental Contamination

Cited by 21 publications

References 28 publications

Maternal and Gestational Factors and Micronucleus Frequencies in Umbilical Blood: The NewGeneris Rhea Cohort in Crete

Maternal and Gestational Factors and Micronucleus Frequencies in Umbilical Blood: The NewGeneris Rhea Cohort in Crete

Micronuclei in Cord Blood Lymphocytes as a Biomarker of Transplacental Exposure to Environmental Pollutants

Treatment of Pregnant Women with a Betamimetic and Verapamil Increases the Micronuclei Frequency in Umbilical Cord Blood Lymphocytes

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