Monitoring of antioxidant properties of uric acid in humans for a consideration measuring of levels of allantoin in plasma by liquid chromatography

Kanďár, Roman; Žáková, Pavla; Mužáková, Vladimíra

doi:10.1016/j.cca.2005.09.002

Cited by 112 publications

(91 citation statements)

References 28 publications

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“…Allantoin levels have been reported as a sensitive marker of oxidative stress in multiple studies [9,10,27]. The data reported here show that allantoin might also represent a novel biomarker for gut inflammation, involving oxidative stress, in the mdr1a -/-gene targeted mutant mouse model.…”

Section: Discussionmentioning

confidence: 50%

“…Critically, allantoin has been reported as a sensitive marker of oxidative stress [9,10]. In most mammals uric acid is converted to allantoin by urate oxidase; however, in humans the urate oxidase gene is not expressed due to several "nonsense" mutations and uric acid is converted to allantoin via reactive oxygen species as shown in Fig. (1) [9,11].…”

Section: Introductionmentioning

confidence: 99%

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Allantoin as A Biomarker of Inflammation in an Inflammatory Bowel Disease Mouse Model: NMR Analysis of Urine

Dryland¹,

Love²,

Walker³

et al. 2008

Open Bioact Compd J

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Crohn's disease (CD) is an Inflammatory Bowel Disease (IBD) that is characterised by destructive inflammation of the intestinal wall. Current methods for determining inflammation of the bowel are costly, time consuming and can cause discomfort to the patients. In order to address these problems, biomarker analysis of more accessible tissues is receiving increasing attention. Oxidative stress has been implicated in the promotion of inflammation. Allantoin has recently been reported as a biomarker for oxidative stress in human serum and urine. This paper investigates allantoin as a biomarker of inflammation in a mouse model of CD. Proton nuclear magnetic resonance ( 1 H NMR) spectroscopy was used to analyse allantoin in urine from the mdr1a -/-mouse which is a model of CD. The data show that the levels of allantoin are strongly correlated with histological injury scores of mouse colonic tissue samples. Allantoin appears to be a useful biomarker of gut inflammation, involving oxidative stress, in a mouse model of CD and may be a potential biomarker in human CD studies.

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Section: Discussionmentioning

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Allantoin as A Biomarker of Inflammation in an Inflammatory Bowel Disease Mouse Model: NMR Analysis of Urine

Dryland¹,

Love²,

Walker³

et al. 2008

Open Bioact Compd J

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“…Uric acid is the end product of purine metabolism in humans [9]. Under physiological conditions, uric acid is a powerful antioxidant and scavenger of ROS/RNS found in the cytosol of the cell and in the extracellular spaces [17].…”

Section: Allantoinmentioning

confidence: 99%

“…Allantoin is a promising biomarker of systemic oxidative status in humans because concentration of allantoin does not depend on variations of uric acid level [9]. Moreover, allantoin is stable regardless of the storage or sample preparation, and additionally it is easily detected in biological material of human samples.…”

Section: Introductionmentioning

confidence: 99%

Today’s oxidative stress markers

Czerska¹,

Mikołajewska²,

Zieliński³

et al. 2015

Med Pr

152

103

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Oxidative stress represents a situation where there is an imbalance between the reactive oxygen species (ROS) and the availability and the activity of antioxidants. This balance is disturbed by increased generation of free radicals or decreased antioxidant activity. It is very important to develop methods and find appropriate biomarkers that may be used to assess oxidative stress in vivo. It is significant because appropriate measurement of such stress is necessary in identifying its role in lifestyle-related diseases. Previously used markers of oxidative stress, such as thiobarbituric acid reactive substances (TBARS) or malondialdehyde (MDA), are progressively being supplemented by new ones, such as isoprostanes (IsoPs) and their metabolites or allantoin. This paper is focusing on the presentation of new ones, promising markers of oxidative stress (IsoPs, their metabolites and allantoin), taking into account the advantage of those markers over markers used previously. Med Pr 2015;66(3):393-405Key words: isoprostanes, oxidative stress, oxidative stress markers, allantoin, metabolites of isoprostanes StreszczenieStres oksydacyjny jest stanem braku równowagi między działaniem reaktywnych form tlenu (RFT) a działaniem antyoksydantów. Równowaga ta może być zakłócona w wyniku zwiększonego działania wolnych rodników lub spadku aktywności antyoksydacyjnej. Zaburzenia te mogą występować zarówno na poziomie komórkowym, jak i całego organizmu. Ponieważ stres oksydacyjny może być podłożem wielu zespołów chorobowych, niezwykle istotne jest znalezienie odpowiednich markerów, które mogą być wykorzystane do oceny jego poziomu in vivo. Stosowane od wielu lat markery -ocenę stężenia aldehyd dimalonowy (MDA) i substancji reagujących z kwasem tiobarbiturowym (thiobarbituric acid reactive substances -TBARS) -stopniowo uzupełnia się nowymi, takimi jak alantoina czy izoprostany (IzoP) wraz z ich metabolitami (IzoP-M). W niniejszej pracy skupiono się na zaprezentowaniu nowych, obiecujących markerów stresu oksydacyjnego (alantoina, IzoP, IzoP-M), ukazując korzyści wynikające z ich stosowania i prognozując dalsze kierunki badań nad ich zastosowaniem. Med. Pr. 2015;66(3):393-405 Słowa kluczowe: izoprostany, stres oksydacyjny, markery stresu oksydacyjnego, alantoina, metabolity izoprostanów Corresponding author / Autorka do korespondencji: Marta Czerska,

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“…Therefore, UA is excreted in urine, and plasma UA levels in humans are appreciably higher as compared to those in most mammals. Studies in vitro have pointed out the important role of uric acid as an antioxidant substance, acting as a potent scavenger of singlet oxygen and hydroxyl radicals and as a chelator of transitional metal ions which are thus converted to poorly reactive forms [12,15].…”

Section: Introductionmentioning

confidence: 99%

Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV

Ferin

Pavão

Baptista

2013

Clinical Biochemistry

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This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. Objectives: Ascorbic (AA) and uric (UA) acids act as antioxidants and are capable to react with biologically relevant oxidants. We aimed to developed a simple, rapid, sensitive, and accurate ion-exclusion HPLC-UV methodology for the simultaneously determination of AA and UA in human plasma.Methods: Analytical pre-requisites, such as the use of heparin as an anticoagulant and meta-phosphoric acid as a stabilizer were added for accurate and reliable measurements. Chromatographic separation was achieved by an isocratic elution on a HEMA-BIO 1000 SB analytical column using a phosphate buffer, pH 2.4, as a mobile phase.Results: Results indicated an excellent linearity with correlation coefficients (r 2 ) ≥ 0.999. The LOD of AA and UA was 1.02 and 1.42 nmol/mL, respectively, while LOQ ranged from 0.306 to 0.426 nmol/mL. A great repeatability for both antioxidants was found, where the CV (%) values for intra-day were lower than 1.8% and under 6.5% for the inter-day assay. The recovery of AA ranged from 92% to 96% and from 99% to 100% for UA.Conclusion: This validated method allows the determination of both antioxidants within 10 min, and is well suited to routine measurements and/or high-throughput clinical analysis. The methodology was applied to assess the antioxidant status of a group of Azorean subjects.

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Monitoring of antioxidant properties of uric acid in humans for a consideration measuring of levels of allantoin in plasma by liquid chromatography

Cited by 112 publications

References 28 publications

Allantoin as A Biomarker of Inflammation in an Inflammatory Bowel Disease Mouse Model: NMR Analysis of Urine

Allantoin as A Biomarker of Inflammation in an Inflammatory Bowel Disease Mouse Model: NMR Analysis of Urine

Today’s oxidative stress markers

Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV

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