Monitoring and biochemical characterization of beta-cypermethrin resistance in  Spodoptera exigua  (Lepidoptera: Noctuidae) in Sichuan Province, China

Wang, Xuegui; Xiang, Xiaoling; Yu, Haoqiang; Liu, Shuhua; Yin, Yong; Cui, Peng; Wu, Yaqiong; Yang, Jing; Jiang, Chengyao; Yang, Qiang

doi:10.1016/j.pestbp.2018.02.008

Cited by 49 publications

(33 citation statements)

References 40 publications

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“…The larvae live inside the cylindrical leaves that give them more protection. In addition, the development of resistance may result in failure in control if insecticides are not appropriately used (Wibisono et al, 2007;Osorio et al, 2008;Ahmad & Arif, 2010;Istiaq et al, 2012;Che et al, 2013;Ahmad et al, 2018;Wang et al, 2018;Saeed et al, 2019). Generally, the farmers identified S. exigua based on the colors of larvae.…”

Section: Resultsmentioning

confidence: 99%

Farmers’ Practices in Using Insecticides to Control Spodoptera exigua Infesting Shallot Allium cepa var. aggregatum in the Shallot Production Centers of Java

Aldini

Trisyono

Wijonarko

et al. 2020

JPTI

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The beet armyworm, Spodoptera exigua Hübn. (Lepidoptera: Noctuidae) is a major insect pest of shallot in Java. This research was aimed to determine how insecticides were used as the common farmers practice for controlling S. exigua. The research was conducted in three shallot production centers in Java: the Districts of Brebes (Central Java Province), Nganjuk (East Java Province), and Bantul (the Special Region of Yogyakarta). Surveys were conducted by interviewing thirty shallot farmers as respondents from each district. The selected thirty farmers were taken from four sub-districts. Almost all farmers (93.3%) in the three districts had similar perception that S. exigua was the major insect pest in shallot and 84.4% farmers stated that this insect was difficult to control. The four most common insecticides active ingredients used were chlorfenapyr, methomyl, chlorpyrifos, and emamectin benzoate. Insecticides remained the first choice and they were applied throughout the shallot season mostly based on the calendar (1-3 days interval). When using insecticides farmers tend to exceed the label recommended rates, and the farmers mostly mixed different insecticides into one spray solution. These results suggest that application of insecticides to control S. exigua was already exessive. The potential risks and efforts essential to minimize the risks are discussed.

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Section: Resultsmentioning

confidence: 99%

Farmers’ Practices in Using Insecticides to Control Spodoptera exigua Infesting Shallot Allium cepa var. aggregatum in the Shallot Production Centers of Java

Aldini

Trisyono

Wijonarko

et al. 2020

JPTI

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“…The exogenous stressors generate reactive oxygen species (ROS), which leads to disturbance of redox reactions and oxidative damages of macromolecules in the organisms (Ali, Rashid, Huang, Wong, & Lei, 2017). GST and CarE are the main enzymes involved in the metabolism of exogenous substances (Wang et al, 2018). In addition, the ROS generated gets scavenged by the low-molecular-weight free-radical scavengers and defensive enzymes including PPO, POD, CAT, and SOD (Qin, Lu, Zheng, & Du, 2016).…”

Section: Discussionmentioning

confidence: 99%

Variation in the pH of experimental diets affects the performance ofLymantria dispar asiaticalarvae and its gut microbiota

Zeng

Shi

Guo

et al. 2020

Arch Insect Biochem Physiol

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To study dietary pH effects on Lymantria dispar asiatica larvae and provide a theoretical basis for its control in different forests, phosphate buffers (PBs) of pH 6, 7, and 8 were used to prepare experimental diets. The diet prepared with pH 6 PB was named as DPB6, with pH 8 PB as DPB8, and with pH 7 PB as DPB7 (control). The dietary pH was 5.00 in DPB6, 6.05 in control, and 6.50 in DPB8. After feeding on the diets with different pH values for 84 hr, fourth‐instar caterpillars were randomly collected. Growth and various physiological traits were determined and 16S recombinant DNA sequencing was performed using the intestinal microflora of surviving larvae. Results showed that the mortality was 30% in DPB6, and 10% in DPB8, while no mortality was observed in control. The partial least squares discriminant analyses suggested that diets prepared with PB of different pH resulted in different food intake, amount of produced feces, weight gain, digestive enzyme activities, and antioxidant enzyme activities in larvae. Interestingly, both the highest weight gain and the lowest total antioxidant capacities were seen in control larvae. Results also showed that the larval gut microbiota community structure was significantly affected by dietary pH. Moreover, linear discriminant analysis effect size suggested that the family Acetobacteraceae in control, genus Prevotella in DPB8, and genus Lactococcus, family Flavobacteriaceae, family Mitochondria, and family Burkholderiaceae in DPB6 contributed to the diversity of the larval gut microbial community.

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“…carboxylesterase (CarE) and glutathione S-transferase (GST) are famous for their detoxification. They are indispensable enzymes for insects dealing with exogenous substance (Aranda et al, 2014;Oakley, 2011;Wang et al, 2018). Antioxidant enzymes, which play critical roles in dealing with adverse environmental stress, are determiners of oxidative resistance (H. Chen et al, 2014;Ma et al, 2017;Paithankar, Raghu, & Patil, 2018).…”

Section: Introductionmentioning

confidence: 99%

Ambient temperature‐mediated enzymic activities and intestinal microflora in Lymantria dispar larvae

Zeng

Shi

et al. 2019

Arch Insect Biochem Physiol

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To understand how ambient temperature affect the gypsy moth larvae, and provide a theoretical basis for pest control in different environments. Fourth instar gypsy moth larvae were incubating for 3 hr at 15℃, 20℃, 25℃, 30℃, 35℃, and 40℃, respectively. Afterward, digestive and antioxidant enzyme activities, total antioxidant capacity, and intestinal microflora community were analyzed to reveal how the caterpillars respond to ambient temperature stress. Results showed that both digestive and antioxidant enzymes were regulated by the ambient temperature. The optimum incubation temperatures of protease, amylase, trehalase, and lipase in gypsy moth larvae were 30℃, 25℃, and 20℃, respectively. When the incubation temperature was deviated optimum temperatures, digestive enzyme activities would be downregulated depending on the extent of temperature stress. In addition, glutathione S‐transferase, peroxidase, catalase, and polyphenol oxidase would be activated under a sufferable temperature stress, but superoxide dismutase and carboxylesterase (CarE) would be inhibited. In addition, results showed that the top two abundant phyla were Proteobacteria and Firmicutes. The phylum Firmicutes abundance was decreased and phylum Proteobacteria abundance was increased by ambient temperature stress. Moreover, it suggested that gypsy moth caterpillars at different ambient temperature mainly differed from each other by Escherichia‐Shigella and Bifidobacterium in control, Acinetobacter in T15, and Lactobacillus in T40, respectively.

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Monitoring and biochemical characterization of beta-cypermethrin resistance in Spodoptera exigua (Lepidoptera: Noctuidae) in Sichuan Province, China

Cited by 49 publications

References 40 publications

Farmers’ Practices in Using Insecticides to Control Spodoptera exigua Infesting Shallot Allium cepa var. aggregatum in the Shallot Production Centers of Java

Farmers’ Practices in Using Insecticides to Control Spodoptera exigua Infesting Shallot Allium cepa var. aggregatum in the Shallot Production Centers of Java

Variation in the pH of experimental diets affects the performance ofLymantria dispar asiaticalarvae and its gut microbiota

Ambient temperature‐mediated enzymic activities and intestinal microflora in Lymantria dispar larvae

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