volume 66, issue 9, P5329-5337 1992
DOI: 10.1128/jvi.66.9.5329-5337.1992
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Abstract: We have cloned Moloney murine sarcoma virus (MuSV) MuSVtsllO DNA by assembly of polymerase chain reaction (PCR)-amplified segments of integrated viral DNA from infected NRK cells (6m2 cells) and determined its complete sequence. Previously, by direct sequencing of MuSVts1lO RNA transcribed in 6m2 cells, we established that the thermosensitive RNA splicing phenotype uniquely characteristic of MuSVtsllO results from a deletion of 1,487 nucleotides of progenitor MuSV-124 sequences. As anticipated, the sequence ob…

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