Molecular typing of Toxoplasma gondii strains by GRA6 gene sequence analysis

Fazaeli, Asghar; Carter, P.E.; Dardé, Marie‐Laure; Pennington, T. H.

doi:10.1016/s0020-7519(00)00036-9

Cited by 132 publications

(115 citation statements)

References 21 publications

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“…Sequence alignment revealed that 25 mutant nucleotide positions (0-0.34%) compared to the T. gondii RH strain are lower than that of ROP38 (Xu et al, 2014), GRA5 (Chen et al, 2012), and GRA6 (Fazaeli et al, 2000), and result in 17 amino acid substitutions (0-0.73%) (Figure 1). Our data indicate there are lower rates of variation in the amino acid sequence of the MIC8 gene among different genotypes of T. gondii, suggesting that the MIC8 protein may be a potential vaccine candidate against acute and chronic T. gondii infections, which has been demonstrated in our previous studies (Liu et al, 2010;Li et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Nucleotide variation in the Toxoplasma gondii micronemal protein 8 gene

Song

Wang

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Toxoplasma gondii is a successful opportunistic protozoan distributed worldwide, which can infect all vertebrates, leading to serious infection, blindness, and abortion. Micronemal (MIC) proteins are critically important for T. gondii infection, as they participate in various stages of the Toxoplasma life cycle, including invasion and attachment to host cells. MIC8 secretion relies on the concentration of intracellular calcium, and can mediate the invasion of T. gondii by interacting with soluble MIC3. To investigate genetic diversity of the MIC8 gene, 16 T. gondii strains from different hosts and geographical locations, and two reference isolates (ToxoDB: TGME49_245490 and TGVEG_245490) were examined in this study. The results showed that all the examined MIC8 genes are 2055 bp, with an A+T content ranging from 50.2 to 50.6%. Conversely, lower levels of variation were detected within their nucleotide and amino acid sequences. Phylogenetic analyses indicated that three classical genotypes of T. gondii and the ToxoDB#9 genotype did not group exclusively via Bayesian inference, maximum parsimony, neighbor joining, and/or maximum likelihood assays based on the nucleotide and amino acid sequences of the MIC8 gene. In summary, the T. gondii MIC8 gene is not a suitable marker for population genetic studies of this parasite.

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Section: Resultsmentioning

confidence: 99%

Nucleotide variation in the Toxoplasma gondii micronemal protein 8 gene

Song

Wang

et al. 2016

Genet. Mol. Res.

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“…PCR had positive results with two of the 100 blood samples from renal transplant recipients group but there were no positive results with those of the control group samples. The size of the PCR products was around 800 base pair (bp) (20) (Figure 1). …”

Section: Resultsmentioning

confidence: 99%

“…It is rare but potentially life-threatening in patients with immune-depression, such as transplant patients (22). In these patients, transmission of T. gondii from a seropositive donor to a seronegative recipient is a significant potential cause of the disease (20,21). Toxoplasmosis in renal transplantation can cause mortality in up to 65% of recipients (15).…”

Section: Discussionmentioning

confidence: 99%

“…Target of the GRA6 gene was amplified with specific primers, forward primer, 5'-GTAGCGTGCTTGTTGGC-GAC-3' and reverse primer, 5'-ACAAGACATAGAGTGCCCC-3' (20). Amplification was carried out with 10 pmol of each primer and 10 µl of extracted DNA in 20 ml of modified Taq DNA polymerase Master Mix RED reaction (Bioneer Korea) containing 75 mM Tris-HCl (PH 8.5), 20 mM (NH4)2SO4, 1.5 mM MgCl2, 0.1% Tween 20, 0.2 mM dNTPs, 0.025 U/µL Amplicon Taq DNA polymerase, inert red dye and a stabilizer.…”

Section: Pcr Amplificationmentioning

confidence: 99%

“…Amplification was carried out with 10 pmol of each primer and 10 µl of extracted DNA in 20 ml of modified Taq DNA polymerase Master Mix RED reaction (Bioneer Korea) containing 75 mM Tris-HCl (PH 8.5), 20 mM (NH4)2SO4, 1.5 mM MgCl2, 0.1% Tween 20, 0.2 mM dNTPs, 0.025 U/µL Amplicon Taq DNA polymerase, inert red dye and a stabilizer. The PCR conditions were 5 minutes at 95°C followed by 35 cycles of 30 seconds at 94°C, 1 minute at 60°C, 2 minutes at 72°C, and a final elongation of 72°C for 7 minutes (20). The PCR products were detected in 1.5% ethidium-bromide-stained agarose gels.…”

Section: Pcr Amplificationmentioning

confidence: 99%

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Detection of Toxoplasmosis in Renal Transplant Recipients by ELISA and PCR Methods in Ahvaz, South-West of Iran

Soltani

Khademvatan

Saki

et al. 2013

Jundishapur J Microbiol

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Background:Toxoplasmosis is an opportunistic infection caused by Toxoplasma gondii. That is asymptomatic in the majority of patients, but it can be life threatening in immune-compromised subjects, such as patients with HIV and organ transplant recipients. Objectives: This study aimed to determine the Toxoplasmosis in Renal Transplant Recipients in Ahvaz, South-West of Iran. Materials and Methods: A total of 100 patients and 100 healthy subjects participated in this study. The specific IgG and IgM antibodies of T. gondii were detected in the two groups by ELISA and the presence of T. gondii in the blood samples was evaluated by GRA6 PCR. Results: 34 (34%) of the renal transplant recipients and 26 (26%) of the control group were positive for anti-Toxoplasma IgG. In addition 18 (18%) of the renal transplant recipients and 4 (4%) of the control group were positive for anti-Toxoplasma IgM. For both values of the antibodies, differences were statistically significant (P < 0.025 and P < 0.001 respectively). The PCR results indicated the presence of T. gondii parasite in 2 blood samples of recipients, but not in those of the healthy control. Conclusions: According to the findings of the current study, renal transplant recipients have a higher prevalence of toxoplasmosis than healthy people and also there is disseminated infection in the patients. Therefore to prevent the severe complications screening and follow up of renal transplant recipients for toxoplasmosis should not be neglected.

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Toxoplasmosis

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Foodborne Parasites

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Molecular typing of Toxoplasma gondii strains by GRA6 gene sequence analysis

Cited by 132 publications

References 21 publications

Nucleotide variation in the Toxoplasma gondii micronemal protein 8 gene

Nucleotide variation in the Toxoplasma gondii micronemal protein 8 gene

Detection of Toxoplasmosis in Renal Transplant Recipients by ELISA and PCR Methods in Ahvaz, South-West of Iran

Toxoplasmosis

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