Molecular typing of nosocomial enterococci by pulsed-field gel electrophoresis

Güldemir, Dilek; Karagöz, Alper; Dal, Tuba; Tekin, Alicem; Özekinci, Tuncer; Durmaz, Rıza

doi:10.5505/turkhijyen.2015.94695

Cited by 5 publications

(5 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In accordance with our results, polyclonal spread of VRE strains was reported in Tunisia 3 and Australia. 17 In addition, several other studies have reported single clone outbreaks in Turkey 11,13 and Italy. 37 The retrospective, monocentric study design and small sample size constitute our limitations.…”

Section: Dovepressmentioning

confidence: 97%

“…To determine the clonal relationship among isolates, PFGE typing was conducted using a previously described method 11 with some modifications. In brief, the samples were digested with the SmaI restriction enzyme (New England Biolabs) and separated by electrophoresis using the CHEF-DR ® II Pulsed-Field Electrophoresis System (Bio-Rad Laboratories, Nazareth, Belgium) with the following parameters: duration, 20 h; temperature, 14°C; first shot duration, 3.5 s; end shot duration, 23.5 s; shot angle, 120°; and current, 6 V/cm 2 .…”

Section: Detection Of Clonal Relationship Among Isolatesmentioning

confidence: 99%

See 1 more Smart Citation

<p>Antibiotic Resistance and Molecular Epidemiology of Vancomycin-Resistant Enterococci in a Tertiary Care Hospital in Turkey</p>

Aşgın

Otlu

2020

IDR

View full text Add to dashboard Cite

Purpose: Vancomycin-resistant enterococci (VRE) have become a global health threat in the last two decades. In this study, we aimed to determine antibiotic resistance using phenotypic and genotypic methods in VRE strains obtained from inpatients and to investigate clonal relatedness among strains. Methods: Identification and antibiotic susceptibility of 47 VRE strains obtained from inpatients at Karabuk University Hospital from 2014 to 2015 were determined using the BD Phoenix™ automated microbiology system. Vancomycin resistance genes (Van A and B) were detected by polymerase chain reaction. Clonal relatedness among the strains was evaluated by pulsed-field gel electrophoresis (PFGE). Results: All 47 VRE strains obtained from rectal (n=35), blood (n=7), and urine (n=5) samples were confirmed as Enterococcus faecium; they were resistant to ampicillin, gentamicin, vancomycin, and teicoplanin. One E. faecium isolate was intermediately resistant to linezolid. No strain was resistant to quinupristin-dalfopristin or daptomycin. Only vanA was detected among strains. According to the PFGE results, 31 of 47 strains were clonally related with a clustering rate of 66%. No common clone was detected. Conclusion: VRE infections are associated with high mortality, morbidity, and healthcare expenditures. Increasing resistance to last-line drugs, such as linezolid and daptomycin, among VRE strains is a great concern. Therefore, comprehensive measures should be performed to reduce VRE colonization. Although there was no common clone VRE outbreak, polyclonal spread was observed in our hospital. The high clustering rate indicated cross-contamination. Thus, a more effective infection control program should be implemented.

show abstract

Section: Dovepressmentioning

confidence: 97%

Section: Detection Of Clonal Relationship Among Isolatesmentioning

confidence: 99%

<p>Antibiotic Resistance and Molecular Epidemiology of Vancomycin-Resistant Enterococci in a Tertiary Care Hospital in Turkey</p>

Aşgın

Otlu

2020

IDR

View full text Add to dashboard Cite

show abstract

“…In Turkey, for the first time, vancomycin resistant Enterococcus faecium (VREfae) was isolated from pleural fluid taken from 11-month-old boy with bronchopulmonary infection, empirically treated with vancomycin and amikacin in 1998 by Vural et al (4). In following years, incidence of these microorganisms, especially VREfae, were increasingly reported from different settings and clinical cases in Turkey (5)(6)(7). Although enterococci are known to have intrinsic resistance to certain antimicrobials such as β-lactams, aminoglycosides; recently, vancomycin resistance together with high level of ampicillin and aminoglycoside resistance have frequently been reported, and today is a major cause of concern due to the limited antimicrobial treatment options (8,9).…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Patients Admitted to the Intensive Care Unit of Hatay State Hospital

Sakin¹,

Aslantaş²,

Bağcı³

2019

tybd

View full text Add to dashboard Cite

Bu çalışmada, Hatay Devlet Hastanesi yoğun bakım ünitesine başvuran hastalardan izole edilen 23 vankomisine dirençli Enterococcus faecium (VREfae) suşunun vankomisine olan direnç mekanizmalarının, virülans genlerinin ve klonal ilişkilerinin belirlenmesi amaçlandı. Gereç ve Yöntem: İzolatların vankomisin minimal inhibitör konsantrasyon (MİK) değerleri E-testi ile antimikrobiyallere olan duyarlılıkları ise disk difüzyon yöntemi ile saptandı. VREfae izolatları arasında klonal ilişki pulsed-field jel elektroforezi (PFGE) ile belirlendi. Vankomisin direnç (vanA, vanB, vanC, vanD, vanE ve vanG) ve virülans genleri (asa1, esp, gelE, hyl ve cylA) polimeraz zincir reaksiyonu (PZR) yöntemi ile araştırıldı. Bulgular: Bütün izolatlar vanA geni taşıdığı tespit edildi ve izolatların 17'si (%73,9) en az bir virülans geni yönünden pozitif bulundu. En yaygın virülans geni olan esp, dokuz izolatta tek başına ve iki izolatta ise hly geni ile birlikte saptandı. hly geni tek başına beş izolatta tespit edilirken ve incelenen diğer virülans genleri (asa1, gelE ve cylA) ise hiçbir izolatta tespit edilmedi. İzolatların tamamı çoğul direnç fenotipi gösterdi. İzolatların vankomisin MİK değerleri E-test ile ≥256 µg/mL olarak bulundu.

show abstract

“…They stated that the genetic similarity observed between VRE fm isolates suggested that there was cross-contamination in the hospital. In the study of Güldemir D. et al [22]. E. faecium isolates formed one main group and four clusters, and 10 E. faecalis strains formed one major group and three clusters.…”

Section: Discussionmentioning

confidence: 93%

An Outbreak of Vancomycin-Resistant Enterococci in a City Hospital Intensive Care Unit: Molecular Characterization of Resistance

Şenol,

Tanrıverdi,

Aşçı Toraman

et al. 2023

Preprint

View full text Add to dashboard Cite

Vancomisin resistant enterococcus(VRE), is a resistant microorganism which colonizates and causes infections in hospitalized patients. It was aimed to show the spread of vancomycin-resistant Enterococcus (fVREfm) step-by-step in all intensive care units, which started with the growth of vancomycin-resistant Enterococcus faecium (VREfm) on 02.12.2021 in the blood culture of a patient hospitalized in the anesthesia intensive care unit of our hospital and was found to have reached epidemic size in the surveys. Rectal swab samples were taken from all patients hospitalized in intensive care units, VRE colonization was determined and the VanA and VanB resistance genes associated with vancomycin resistance of VREfm isolates were determined by PCR method, and clonal association analysis was performed by Arbitrarily Primed-PCR (AP-PCR) and Pulsed-Field Gel Electrophoresis. In our study, VRE was detected in 61 of 2601 rectal swab samples. 54 (85.52%) of the VRE isolates were Enterococcus faecium, 3 (4.91%) was Enterococcus faecalis, 3 (4.91%) was Enterococcus gallinorum, 1 (1.63%) was Enterococcus casseliflavus. It was determined that all of the 54 VREfm isolates, which were the most detected among all VRE isolates, carried the vanA gene. In the clonal association analysis of the isolates by AP-PCR and PFGE methods, it was found that they had 12 different genotypes, 48 of them were included in any cluster, the clustering rate was 88.8%, and the largest cluster was genotype 1 cluster with 36 isolates. Of the 54 patients with VREfm isolated recently, 18.51 percent of the clinical samples were isolated before the survey, and 9.24% were isolated after the survey. It was determined that 100% of VREfm isolates were resistant to ampicillin, levofloxacin, ciprofloxacin, high-level gentamicin, trimethoprim-sulfamethoxazole, teicoplanin, 7.4% to tigecycline and 1.85% to linezolid. In our study, in the clonal association analysis performed by isolating VREfm in rectal swab samples, it was found that 88.8% of the samples were indistinguishably similar and the increase in the number of VREfm infections after the index case in our hospital was associated with the epidemic. VREfm infections cause long term hospitalization, costs and also deaths, which shows the seriousness of the event, and the importance of the combination of epidemiological and molecular analysis in epidemic research.

show abstract

Molecular typing of nosocomial enterococci by pulsed-field gel electrophoresis

Abstract: ABSTRACT

Cited by 5 publications

References 34 publications

<p>Antibiotic Resistance and Molecular Epidemiology of Vancomycin-Resistant Enterococci in a Tertiary Care Hospital in Turkey</p>

<p>Antibiotic Resistance and Molecular Epidemiology of Vancomycin-Resistant Enterococci in a Tertiary Care Hospital in Turkey</p>

Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Patients Admitted to the Intensive Care Unit of Hatay State Hospital

An Outbreak of Vancomycin-Resistant Enterococci in a City Hospital Intensive Care Unit: Molecular Characterization of Resistance

Contact Info

Product

Resources

About