Molecular typing, biofilm production, and detection of carbapenemase genes in multidrug-resistant Acinetobacter baumannii isolated from different infection sites using ERIC-PCR in Hamadan, west of Iran

Abstract

Background Acinetobacter baumannii is an opportunistic pathogen that can cause several kinds of nosocomial infections. Increasing antibiotic resistance as well as identifying genetic diversity and factors associated with pathogenicity and prevalence of this bacterium is important. The aim of this study was the investigation of molecular typing, biofilm production, and detection of carbapenemase genes in multidrug-resistant Acinetobacter baumannii isolated from different infection sites using ERIC-PCR in Iran. Methods Forty isolates of A. baumannii were obtained from various wards of the central hospital, in the west of Iran. Phenotypic identification and genetic diversity, biofilm production assay, and detection of Carbapenemase genes carried out. Results Tracheal samples 26 (61.9 %) are the most frequent isolates, and 95 % of isolates were identified as MDR. 32.5 % of all A. baumannii strains were capable to form a strong biofilm. It was founded that antimicrobial resistance patterns had a significant relationship with strong biofilm formation (P = 0.001). Most frequencies of the studied genes were in the order of VIM (81 %), SPM (45.2 %), and IMP (35.7 %) genes. The VIM gene was the most frequent in all isolates which were significant (P = 0.006). 14 different ERIC-types were observed including 7 common types and 7 unique or single types. F type is the largest common type consisting of nine isolates and B, D, and E types contain two isolates separately. Conclusions ERIC-PCR technique was used to genetically classify A. baumannii isolates as one of the most common microorganisms in nosocomial infections.

Virulence Molecular Epidemiology of Clinical Carbapenem-Resistant Acinetobacter baumannii: First Report from Jordan

“…In this study, out of 110 tested CRAB isolates, 86.4% (n=95) were biofilm producers, among which 31 (32.6%) were week biofilm producers, 40 (42.1%) were moderate biofilm producers, and 24 (25.3%) were strong biofilm producers. Similar results were reported previously [47], but still disagreement present with other study that reported different percentages [48]. This variation might be attributed with the widespread prevalence of biofilm-encoding genes among A. baumannii strains, variations of the biofilm-formation assay principles, or environmental factors [49].…”

Unmasking the Resistance: Detecting Carbapenem Genes in Acinetobacter baumannii Isolated from some Hospitals in Najaf and Baghdad

“…In terms of carbapenemase-encoding genes, bla VIM was detected in 18% of the isolates, resembling the findings of [32] but differing significantly from the studies conducted by [33][34][35]. Interestingly, bla SPM was found in 50% of the isolates, contradicting the findings of [34], where none of the tested isolates carried this gene.…”

Genetic Diversity of Acinetobacter Baumannii Strains Isolated in Different Hospitals

“…Among the PCR-based fingerprint techniques, ERIC-PCR is preferred as an economical, simple, and fast method used to identify and distinguish Acinetobacter species [16]. Therefore, it is important to control infections in hospitals and periodically monitor antimicrobial resistance patterns in different regions for infection control [17] In this study, the ERIC-PCR technique was used to see the genetic distribution of A. baumannii strains which cause common nosocomial infections. Overall, the findings of this study showed that the ERIC-PCR method is a viable method to reveal the genetic diversity of different A. baumannii strains.…”