Molecular tracing of Bradyrhizobium strains helps to correctly interpret Acacia mangium response to inoculation in a reforestation experiment in Madagascar

Prin, Yves; Galiana, Antoine; Roux, Christine Le; Méléard, Benoît; Razafimaharo, V.; Ducousso, Marc; Chaix, Gilles

doi:10.1007/s00374-002-0564-3

Cited by 11 publications

(11 citation statements)

References 11 publications

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“…Nonetheless, this is possible, since we isolated the inoculant strain twice in nodules sampled in the non-inoculated field trial at Itatinga, proving that the inoculated strain was able, after short-distance migration, to settle in a new environment and colonize the roots of local A. mangium. The same "invasion" was also observed in Madagascar [49].…”

Section: Artificial Inoculation and Persistence Of The Inoculumsupporting

confidence: 70%

“…Inoculation was carried out in 1999 and 2003 at the Porto Trombetas and Itatinga sites respectively, theoretically leaving enough time for recombination and gene transfer to take place. Several studies have shown that, outside its native area, A. mangium inoculated with native Australian Bradyrhizobium strains generally gives excellent growth responses compared to the local spontaneous bacterial strains [15], although A. mangium promiscuity results in non-inoculated plants being extremely easily colonized by local bacteria in field trials [49]. In our case, the local strains might have been as competitive and efficient as the inoculant strains, at least in terms of nodulation, thereby not favouring symbiotic gene acquisition by local strains.…”

Section: Genic Diversity Recombination and Persistence Of The Inoculummentioning

confidence: 99%

“…In two A. mangium field trials in Ivory Coast in 1994, Galiana et al [16] recovered an inoculated strain 42 months after field transplantation, whereas another one disappeared after 19 months. Martin-Laurent et al [29] and Prin et al [49] analysed nodules from A. mangium 4 and 6 months after transfer to the field in Singapore and Madagascar respectively. Molecular analyses showed the persistence and the overgrowth of one of the two inoculums to the detriment of the other.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of symbiotic Bradyrhizobium elkanii populations recovered from inoculated and non-inoculated Acacia mangium field trials in Brazil

Perrineau

Roux

Faria

et al. 2011

Systematic and Applied Microbiology

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Acacia mangium is a legume tree native to Australasia. Since the eighties, it has been introduced into many tropical countries, especially in a context of industrial plantations. Many field trials have been set up to test the effects of controlled inoculation with selected symbiotic bacteria versus natural colonization with indigenous strains. In the introduction areas, A. mangium trees spontaneously nodulate with local and often ineffective bacteria. When inoculated, the persistence of inoculants and possible genetic recombination with local strains remain to be explored. The aim of this study was to describe the genetic diversity of bacteria spontaneously nodulating A. mangium in Brazil and to evaluate the persistence of selected strains used as inoculants. Three different sites, several hundred kilometers apart, were studied, with inoculated and non-inoculated plots in two of them. Seventy-nine strains were isolated from nodules and sequenced on three housekeeping genes (glnII, dnaK and recA) and one symbiotic gene (nodA). All but one of the strains belonged to the Bradyrhizobium elkanii species. A single case of housekeeping gene transfer was detected among the 79 strains, suggesting an extremely low rate of recombination within B. elkanii, whereas the nodulation gene nodA was found to be frequently transferred. The fate of the inoculant strains varied depending on the site, with a complete disappearance in one case, and persistence in another. We compared our results with the sister species Bradyrhizobium japonicum, both in terms of population genetics and inoculant strain destiny.

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Section: Artificial Inoculation and Persistence Of The Inoculumsupporting

confidence: 70%

Section: Genic Diversity Recombination and Persistence Of The Inoculummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of symbiotic Bradyrhizobium elkanii populations recovered from inoculated and non-inoculated Acacia mangium field trials in Brazil

Perrineau

Roux

Faria

et al. 2011

Systematic and Applied Microbiology

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“…Both theoretical (Parker, 2001) and empirical studies (Prin et al, 2003;Rodríguez-Echeverría et al, 2007;Rodríguez-Echeverría, 2010; this study) suggest that the introduction of inoculants with Australian acacias has been a key factor for the establishment of these species in new areas. Nevertheless, the different degree of invasiveness of Australian acacias might still be related to the symbiotic specificity.…”

Section: Conclusion and Priorities For Future Workmentioning

confidence: 88%

“…How these bacteria got introduced into Europe remains unclear. Either they were intentionally co-introduced during plantation, as for other acacias (Prin et al, 2003), or accidentally introduced attached to seed coats (Perez-Ramirez et al, 1998). Although Australian bradyrhizobia are the predominant symbionts of invasive A. longifolia in Portugal, this species can also nodulate with other bradyrhizobia present in the invaded soils (Rodríguez-Echeverría et al, 2007).…”

Section: The Legume-rhizobia Symbiosis In Australian Acaciasmentioning

confidence: 99%

Jack‐of‐all‐trades and master of many? How does associated rhizobial diversity influence the colonization success of Australian Acacia species?

Rodríguez‐Echeverría

Roux

Crisóstomo

et al. 2011

Diversity and Distributions

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Aim To evaluate the role of rhizobial diversity, and symbiotic promiscuity, on the invasive ability of Australian acacias (Acacia species in subgenus Phyllodineae native to Australia). Location Global. Methods A bibliographic review of the rhizobial diversity associated with Australian Acacia species was performed to assess symbiotic promiscuity for invasive and non‐invasive species. The rhizobial diversity associated with Acacia dealbata and A. saligna in Australia and Portugal and with A. pycnantha in Australia and South Africa was assessed by 16S rDNA and intergenic spacer sequencing of bacteria isolated from field‐collected nodules. Results All studied Australian acacias are nodulated by strains in the genus Bradyrhizobium, which appears to be the dominant group of acacia symbionts in native and non‐native soils. Both literature and experimental data from this study suggest that Australian bradyrhizobia might have been co‐introduced with acacias to new geographical regions. The studied Acacia species can also harbour other root‐nodulating alpha and betaproteobacteria genera, although these are less abundant than Bradyrhizobium. Main conclusions There is no clear difference in the diversity of rhizobial species associated with invasive and non‐invasive Australian acacias. All studied invasive acacias nodulate in both native and non‐native regions, harbouring predominantly Bradyrhizobium strains but showing some degree of symbiotic promiscuity. The co‐introduction of compatible root‐nodulating bacteria from Australia might explain the establishment of invasive populations, but novel associations with rhizobia from the invaded soils are also possible. Invasive legumes might use both strategies but species with low symbiotic promiscuity would become invasive only if compatible bacteria are co‐introduced in the new regions. The progress of invasion and the impacts on the invaded ecosystems might also differ depending on the nodulation strategy.

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Co-inoculation of Acacia mangium with Glomus intraradices and Bradyrhizobium sp. in aeroponic culture

et al. 2005

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Molecular tracing of Bradyrhizobium strains helps to correctly interpret Acacia mangium response to inoculation in a reforestation experiment in Madagascar

Cited by 11 publications

References 11 publications

Genetic diversity of symbiotic Bradyrhizobium elkanii populations recovered from inoculated and non-inoculated Acacia mangium field trials in Brazil

Genetic diversity of symbiotic Bradyrhizobium elkanii populations recovered from inoculated and non-inoculated Acacia mangium field trials in Brazil

Jack‐of‐all‐trades and master of many? How does associated rhizobial diversity influence the colonization success of Australian Acacia species?

Co-inoculation of Acacia mangium with Glomus intraradices and Bradyrhizobium sp. in aeroponic culture

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