Molecular systematics support the revival of Mycobacterium salmoniphilum (ex Ross 1960) sp. nov., nom. rev., a species closely related to Mycobacterium chelonae

Whipps, Christopher M.; Butler, W. Ray; Pourahmad, Fazel; Watral, Virginia; Kent, Michael L.

doi:10.1099/ijs.0.64841-0

Cited by 81 publications

(89 citation statements)

References 31 publications

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“…salmoniphilum was first described in 1960 (65), but the new species was not validated. It was revived in 2007, on the basis of 11 strains isolated from salmonid fish (66).…”

Section: Mycobacterium Salmoniphilummentioning

confidence: 99%

Microbiological Features and Clinical Relevance of New Species of the Genus Mycobacterium

2014

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SUMMARY Nontuberculous mycobacteria (NTM) are present in the environment, mainly in water, and are occasionally responsible for opportunistic infections in humans. Despite the fact that NTM are characterized by a moderate pathogenicity, the diseases caused by NTM at various body sites are increasing on a worldwide level. Among over 150 officially recognized NTM species, only two or three dozen are familiar to clinicians, and even to most microbiologists. In this paper, approximately 50 new species described in the last 8 years are reviewed, and their role in human infections is assessed on the basis of reported clinical cases. The small number of reports concerning most of the “new” mycobacterial species is responsible for the widespread conviction that they are very rare. Their role is actually largely underestimated, mainly because they often remain unrecognized and misidentified. Aiming to minimize such bias, emphasis has been placed on more common identification pitfalls. Together with new NTM, new members of the Mycobacterium tuberculosis complex described in the last few years are also an object of the present review.

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“…salmoniphilum was first described in 1960 (65), but the new species was not validated. It was revived in 2007, on the basis of 11 strains isolated from salmonid fish (66).…”

Section: Mycobacterium Salmoniphilummentioning

confidence: 99%

Microbiological Features and Clinical Relevance of New Species of the Genus Mycobacterium

2014

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“…Shojaei et al 2000, Cousins et al 2003. Aquatic poikilotherms in particular have been a productive source of novel Mycobacterium spp., including M. montefiorense from moray eels Gymnothorax funebris (Levi et al 2003), M. shottsii and M. pseudoshottsii from striped bass Morone saxatilis (Rhodes et al 2003(Rhodes et al , 2005, M. stomatepiae from the cichlid Stomatepia mariae (Pourahmad et al 2008), and M. salmoniphilum from salmonids, a revived species name which was recently differentiated from M. chelonae (Whipps et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Characterization of photochromogenic Mycobacterium spp. from Chesapeake Bay striped bass Morone saxatilis

Gauthier¹,

Helenthal²,

Rhodes³

et al. 2011

Dis. Aquat. Org.

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A large diversity of Mycobacterium spp. has been isolated from striped bass Morone saxatilis in Chesapeake Bay, USA. The new species M. shottsii and M. pseudoshottsii are the dominant isolates, while the classical fish pathogen M. marinum is found much less frequently. M. fortuitum and M. chelonae, other Mycobacterium spp. known to commonly infect fishes, have not yet been aseptically isolated from striped bass within Chesapeake Bay. While M. pseudoshottsii and M. shottsii have been phenotypically and genotypically characterized, other less common mycobacterial isolates have not. In the present study, we describe 17 photochromogenic isolates from Chesapeake Bay striped bass using phenotypic characterization and multilocus sequencing of 16S rRNA, hsp65 and rpoB genes. Genetic characterization reveals that these isolates are related to widely divergent portions of the mycobacterial phylogeny; however, some interesting trends are observed, such as a majority of isolates (10/17) belonging to the M. simiae-related grouping. Five additional isolates were assigned to the slow-growing mycobacteria (including 2 identified as M. marinum), while 2 are clearly shown to belong genetically to the fast-growing mycobacteria.

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“…salmoniphilum, a new Mycobacterium chelonae-like organism (Whipps et al, 2007). However, using overlapping primers to Tb11 and Tb12, F927 and R927, those two isolates were easily amplified and sequenced.…”

Section: Resultsmentioning

confidence: 99%

Comparative evaluation of Polymerase Chain Reaction–Restriction Enzyme Analysis (PRA) and sequencing of heat shock protein 65 (hsp65) gene for identification of aquatic mycobacteria

Pourahmad

Thompson

Adams

et al. 2009

Journal of Microbiological Methods

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Traditional identification of mycobacteria based on cultural and biochemical tests can take several weeks and may fail to provide a precise identification. Polymerase Chain Reactionrestriction analysis (PRA) of the gene encoding heat shock protein 65 kDa (hsp65) gene has been proposed as a rapid and inexpensive alternative approach. Despite being widely used for differentiation of mammalian mycobacteria, this method has only been applied in the identification of a small number of aquatic mycobacteria. The present study aimed to evaluate the potential use of PRA of hsp65 for the identification of aquatic mycobacteria compared with sequence analysis. Seventy one mycobacterial isolates including, 10 type/ reference strains and the remainder field isolates, were subjected to PRA of a 441 bp fragment of this gene. For 68 representative isolates, sequence analysis was performed. All rapidly and slowly growing mycobacteria had best matches with 99.3% to 100% similarity with their corresponding species in the databanks.PRA proved to be a simple and rapid method for identifying aquatic mycobacteria.However, the incidence of similar or identical restriction patterns for some species of mycobacteria, and in particular, identification of new species of mycobacteria is a major problem using such a method. In contrast, the nucleic acid sequencing of the hsp65 gene yielded unambiguous results. Mycobacteriosis (fish tuberculosis) is a progressive disease of a wide range of wild and captive marine and freshwater fish species (Chinabut, 1999). The zoonotic nature of the organism, the massive economical losses which have occurred to the aquaculture industry due to this disease and the lack of effective treatment highlight the need for mycobacteria to be rapidly detected and identified to species level. IntroductionTraditional identification of mycobacteria based on cultural and biochemical tests may take several weeks, and sometimes even then fail to provide a precise identification. In addition, the procedures of these tests are time consuming and complicated. Therefore, various molecular based methods have been developed for the rapid detection and identification of mycobacteria species. There are currently several commercially available methods, including the AccuProbe system (Genprobe, San Diego, Calif), INNO-LiPA (Innogenetics, Ghent, Belgium), or GenoType Mycobacterium assay (Hain, Lifescience, Germany). Although such tests provide desirable sensitivity and specificity, they are limited to the identification of a small number of mycobacteria. Among other molecular methods, Polymerase Chain Reaction (PCR) -RestrictionEnzyme Analysis (PRA), and sequence analysis have been applied to several mycobacteria genes. In this regard, PCR of the 441 bp of heat shock protein 65 kD gene (hsp65), followed by restriction fragment pattern analysis by BstEII and HaeIII enzymes proposed by Telenti et al. (1993), has provided a rapid method for identifying mycobacteria and closely related species (Brunello et al., 2001; Devallois et ...

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Molecular systematics support the revival of Mycobacterium salmoniphilum (ex Ross 1960) sp. nov., nom. rev., a species closely related to Mycobacterium chelonae

Cited by 81 publications

References 31 publications

Microbiological Features and Clinical Relevance of New Species of the Genus Mycobacterium

Microbiological Features and Clinical Relevance of New Species of the Genus Mycobacterium

Characterization of photochromogenic Mycobacterium spp. from Chesapeake Bay striped bass Morone saxatilis

Comparative evaluation of Polymerase Chain Reaction–Restriction Enzyme Analysis (PRA) and sequencing of heat shock protein 65 (hsp65) gene for identification of aquatic mycobacteria

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