Molecular Survey of Anaplasmataceae Agents and Coxiellaceae in Non-Hematophagous Bats and Associated Ectoparasites from Brazil

Ikeda, Priscila; Torres, Jaire Marinho; Placa, Ana Julia Vidal; Mello, Victória Valente Califre de; Lourenço, Elizabete Captivo; Herrera, Heitor Miraglia; Oliveira, Carina Elisei de; Hemsley, Claudia; Titball, Richard W.; Machado, Rosangela Zacarias; André, Marcos Rogério

doi:10.3390/parasitologia1040021

Cited by 15 publications

(4 citation statements)

References 94 publications

(140 reference statements)

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“…Therefore, the primers and probes used might have not been able to hybridize to groEL gene of the putative novel genotypes of Ehrlichia and Anaplasma found in this body of work. Similar results were found when screening biological samples of armadillos, sloths, anteaters (Calchi et al, 2020), and bats (Ikeda et al, 2021) for Ehrlichia and Anaplasma using this qPCR protocol. On the other hand, Ehrlichia and Anaplasma genotypes were detected in biological samples from wild rodents (Benevenute et al, 2017) and wild birds (Sacchi et al, 2021) using the referred qPCR assay.…”

Section: 54supporting

confidence: 78%

Molecular detection of vector‐borne agents in wild boars ( Sus scrofa ) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia , Anaplasma , and haemoplasmas

Santana

Hoppe

Carraro

et al. 2022

Transbounding Emerging Dis

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The present study aimed to investigate, by molecular techniques, the occurrence of Anaplasmataceae, Bartonellaceae, Rickettsiaceae, Mycoplasmataceae, Coxiellaceae, and Babesiidae/Theileriidae agents in blood samples of free-living wild boars (Sus scrofa) and associated ticks in south-eastern Brazil. For this purpose, 67 blood samples and 265 ticks (264 Amblyomma sculptum and one Amblyomma ovale) were analysed.In the screening for Anaplasmataceae agents by a PCR assay based on the 16S rRNA gene, 5.97% blood samples and 50.54% ticks were positive. In the PCR assay for Ehrlichia spp. based on the dsb gene, 9.24% of ticks were positive. Despite the low occurrence, a possible new 16S rRNA genotype of Anaplasma sp. was detected in a wild boar's blood sample. According to phylogenetic analyses based on the 16S rRNA, gltA, and sodB genes and ITS (23S-5S rRNA) intergenic region, it was found that A. sculptum and A. ovale ticks collected from wild boars carry Ehrlichia genotypes phylogenetically associated with Ehrlichia ewingii, Ehrlichia ruminantium, and new Ehrlichia genotypes previously detected in horses, peccaries, and ticks collected from jaguars.In the screening for haemoplasmas by a qPCR based on the 16S rRNA gene, 88.06% of blood samples and 8.69% of ticks were positive. Mycoplasma suis, Mycoplasma parvum, and a possible new haemoplasma genotype were detected in wild boars in southeastern Brazil. In the screening for Bartonella spp. using a nuoG-based qPCR assay, 3.8% of tick samples were positive. Phylogenetic inferences positioned four nuoG and one r gltA Bartonella sequences into the same clade as Bartonella machadoae. No blood or tick samples from wild boars showed to be positive in the qPCR for Coxiella burnetii based on the IS1111 gene. On the other hand, only 1.6% of ticks were positive in the e2808

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Section: 54supporting

confidence: 78%

Molecular detection of vector‐borne agents in wild boars ( Sus scrofa ) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia , Anaplasma , and haemoplasmas

Santana

Hoppe

Carraro

et al. 2022

Transbounding Emerging Dis

Self Cite

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“…In 2020, in the regions of Pernambuco and Ceará, one rodent (Wiedomys pyrrhorhinos) and one marsupial (Didelphis albiventris) were seroreactive to C. burnetii at endpoint titers of 128 and 4,096, respectively, by IFA test, no phase I or phase II specification (Oliveira et al, 2020). Finally, in 2021, Mato Grosso do Sul, Ikeda et al (2021) sampled 135 non-hematophagous bats and did not find real-time PCR positivity for C. burnetii based on the IS1111 gene. Therefore, bats do not act as important hosts in epidemiology or C. burnetii in Brazil.…”

Section: Situation In Brazilmentioning

confidence: 99%

Q fever and coxiellosis in Brazil: an underestimated disease? A brief review

Souza

André

Labruna

et al. 2022

Rev. Bras. Parasitol. Vet.

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Q fever, caused by the γ-proteobacterium Coxiella burnetii, is a zoonosis of great importance and global impact. This agent has high transmissibility and can spread over long distances via wind, in which a small number of aerosolized particles are needed to infect susceptible hosts. The clinical diagnosis of Q fever is difficult owing to the variety of clinical signs shared with other diseases. In Brazil, studies related to C. burnetii are constantly being conducted, and this review aims to increase the number of approaches already studied, leading to the following question: is Q fever an unknown, neglected disease, or does it have a focal occurrence in certain areas (exotic/rare) in the country?

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“…Clearly, a broad diversity of bat-associated bacterial pathogens are likely infecting humans, but the link to bats is often not recognized. are often detected in ectoparasites of bats, such as Anaplasma and Ehrlichia in ticks, mites, and bat fies [21,[89][90][91][92]; Bartonella in bat fies, feas, ticks, mites, and bat bugs [21,23,60,63,80,85,93,104]; Borrelia fainii, Borrelia johnsonii, Borrelia miyamotoi, and other Borrelia spp. in ticks [29,85,86,92,[105][106][107]; Coxiella in ticks [108]; Mycoplasma in bat fies and ticks [21,26,109]; and Rickettsia in bat fies, feas, mites, and ticks [21,85,90,92,95,104,107,[110][111][112][113][114][115][116][117][118].…”

Section: Zoonotic Potential Of Bat-associated Bacterial Pathogensmentioning

confidence: 99%

Trends in Bacterial Pathogens of Bats: Global Distribution and Knowledge Gaps

Szentiványi

McKee

Jones

et al. 2023

Transboundary and Emerging Diseases

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Bats have received considerable recent attention for infectious disease research because of their potential to host and transmit viruses, including Ebola, Hendra, Nipah, and multiple coronaviruses. These pathogens are occasionally transmitted from bats to wildlife, livestock, and to humans, directly or through other bridging (intermediate) hosts. Due to their public health relevance, zoonotic viruses are a primary focus of research attention. In contrast, other emerging pathogens of bats, such as bacteria, are vastly understudied despite their ubiquity and diversity. Here, we describe the currently known host ranges and geographic distributional patterns of potentially zoonotic bacterial genera in bats, using published presence-absence data of pathogen occurrence. We identify apparent gaps in our understanding of the distribution of these pathogens on a global scale. The most frequently detected bacterial genera in bats are Bartonella, Leptospira, and Mycoplasma. However, a wide variety of other potentially zoonotic bacterial genera are also occasionally found in bats, such as Anaplasma, Brucella, Borrelia, Coxiella, Ehrlichia, Francisella, Neorickettsia, and Rickettsia. The bat families Phyllostomidae, Vespertilionidae, and Pteropodidae are most frequently reported as hosts of bacterial pathogens; however, the presence of at least one bacterial genus was confirmed in all 15 bat families tested. On a spatial scale, molecular diagnostics of samples from 58 countries and four overseas departments and island states (French Guiana, Mayotte, New Caledonia, and Réunion Island) reported testing for at least one bacterial pathogen in bats. We also identified geographical areas that have been mostly neglected during bacterial pathogen research in bats, such as the Afrotropical region and Southern Asia. Current knowledge on the distribution of potentially zoonotic bacterial genera in bats is strongly biased by research effort towards certain taxonomic groups and geographic regions. Identifying these biases can guide future surveillance efforts, contributing to a better understanding of the ecoepidemiology of zoonotic pathogens in bats.

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Molecular Survey of Anaplasmataceae Agents and Coxiellaceae in Non-Hematophagous Bats and Associated Ectoparasites from Brazil

Cited by 15 publications

References 94 publications

Molecular detection of vector‐borne agents in wild boars ( Sus scrofa ) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia , Anaplasma , and haemoplasmas

Molecular detection of vector‐borne agents in wild boars ( Sus scrofa ) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia , Anaplasma , and haemoplasmas

Q fever and coxiellosis in Brazil: an underestimated disease? A brief review

Trends in Bacterial Pathogens of Bats: Global Distribution and Knowledge Gaps

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