Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human

Alsalihi, Saba F; Jawad, Ali; Alrodhan, Mohsen

doi:10.1088/1742-6596/1294/6/062097

Cited by 2 publications

(2 citation statements)

References 20 publications

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“…The human beings are being transmitted due to dromedary camels which are infected previously. MERS-CoVis spreaded Mostly in Middle east countries like Africa, south asia (Alsalihi et al, 2019) [1] .…”

Section: Source Of the Virusmentioning

confidence: 99%

A review on human virus

Evangelin¹,

Kala²,

Bala³

et al. 2020

Int. J. Chem. Stud.

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The Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in human for the first time in the history. This is a zoonotic pathogen which was appeared first in Saudi Arabia and Jordan. According to WHO report, 858 deaths out of 27 countries were reported Between April 2012 and December 2019.The substrate at position P1 in glutamine is important for the protease of corona virus which is similar to that of 3Cprotease in entero viruses. In the development of viral drugs, the best target is proteases having specificity and plays an important role in the processing of viral polyprotein. Based on the structure. Peptidomimetic ketoamides designed as potent, broad spectrum inhibitor of 3c proteases against corona viruses. The present review is focused on the symptoms, transmission, target and prevention of the virus.

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Section: Source Of the Virusmentioning

confidence: 99%

A review on human virus

Evangelin¹,

Kala²,

Bala³

et al. 2020

Int. J. Chem. Stud.

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“…Alsalihi and her colleagues (2019) have reported MERS-CoV infection among Iraqi individuals and camels. Most of the Iraqi variants were grouped in clade B and were closely related to Saudi Arabia and Jordan variants in the phylogenetic tree ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

Expression and purification of MERS-CoV envelope protein, an essential viroporin, using the baculovirus expression system

Alsaadi¹,

Alghezi²,

Jones³

2023

IJM

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Background and Objectives: The causative agent of Middle East Respiratory Syndrome (MERS) is a zoonotic Coronavirus (MERS-CoV) identified in Saudi Arabia in 2012. The envelope (E) protein of MERS-CoV is a small viral protein which plays several essential roles during virus replication. To facilitate study of the structure and function of the E protein, recombinant MERS-CoV E protein was expressed using the baculovirus expression system. Materials and Methods: A recombinant E open reading frame including an 8-histidine tag at the amino terminus was de-signed and cloned into a baculovirus transfer vector. Following construction of a recombinant virus insect cells were infected and the expression of the E protein assessed by SDS-PAGE and Western blotting. Results: Recombinant E protein, tagged at the N-terminus with a polyhistidine sequence, with a molecular mass of 10.18 kD was identified by Western blotting with an anti-His antibody. Following large scale infection E protein was released by detergent mediated lysis of infected cells and purified by Immobilized Metal Ion Affinity Chromatography (IMAC). Conclusion: Purified full length recombinant MERS-CoV E protein can be isolated by IMAC and is suitable for further functional, biophysical or immunological studies.

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Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human

Cited by 2 publications

References 20 publications

A review on human virus

A review on human virus

Expression and purification of MERS-CoV envelope protein, an essential viroporin, using the baculovirus expression system

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