Molecular signatures of phytol-derived immunostimulants in the context of chemokine–cytokine microenvironment and enhanced immune response

Aachoui, Youssef; Chowdhury, Roshni Roy; Fitch, Richard W.; Ghosh, S.

doi:10.1016/j.cellimm.2011.07.001

Cited by 16 publications

(28 citation statements)

References 43 publications

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“…In a previous report, we showed that intraperitoneal immunization with alum, an adjuvant licensed for use in humans, promotes a pro-inflammatory microenvironment characterized by induction of cytokines (IL-4, IL-12p40p70, IL-1α, sTNF R I, sTNF R II) and chemotactic factors (MCP-1, LIX, KC, M-CSF, and TIMP-1) [24]. To assess whether SIS provokes inflammatory reactions, we studied SIS alone in peritoneal exudates 24 hours after i.p.…”

Section: Resultsmentioning

confidence: 99%

Extracellular Matrix from Porcine Small Intestinal Submucosa (SIS) as Immune Adjuvants

Aachoui

Ghosh

2011

PLoS ONE

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Porcine small intestinal submucosa (SIS) of Cook Biotech is licensed and widely used for tissue remodeling in humans. SIS was shown to be highly effective as an adjuvant in model studies with prostate and ovarian cancer vaccines. However, SIS adjuvanticity relative to alum, another important human-licensed adjuvant, has not yet been delineated in terms of activation of innate immunity via inflammasomes and boosting of antibody responses to soluble proteins and hapten-protein conjugates. We used ovalbumin, and a hapten-protein conjugate, phthalate-keyhole limpet hemocyanin. The evaluation of SIS was conducted in BALB/c and C57BL/6 mice using both intraperitoneal and subcutaneous routes. Inflammatory responses were studied by microarray profiling of chemokines and cytokines and by qPCR of inflammasomes-related genes. Results showed that SIS affected cytokine and chemokines microenvironments such as up-regulation of IL-4 and CD30-ligand and activation of chemotactic factors LIX and KC (neutrophil chemotactic factors), MCP-1 (monocytes chemotactic factors), MIP 1-α (macrophage chemotactic factor) and lymphotactin, as well as, growth factors like M-CSF. SIS also promoted gene expression of Nod-like receptors (NLR) and associated downstream effectors. However, in contrast to alum, SIS had no effects on pro-inflammatory cytokines (IL-6, IL-1β, TNF-α) or NLRP3, but it appeared to promote both Th1 and Th2 responses under different conditions. Lastly, it was as effective as alum in engendering a lasting and specific antibody response, primarily of IgG1 type.

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Section: Resultsmentioning

confidence: 99%

Extracellular Matrix from Porcine Small Intestinal Submucosa (SIS) as Immune Adjuvants

Aachoui

Ghosh

2011

PLoS ONE

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“…The differences in their immune profiles could be explained by the presence of different polar moieties, OH vs. Cl on PHIS-01 and PCl respectively. In fact, our previous studies have shown that the terminal polar residue in phytanol plays an important role in shaping the immune response, particularly T-cell polarization [24]. Additionally, physical properties, such as the size of the emulsion droplets in each formulation, could also impact the type of immune response generated.…”

Section: Effects Of Phytol-derived Immunostimulants and Alum On The Imentioning

confidence: 97%

“…Cytokines and chemokines in peritoneal fluids were assessed using the mouse inflammatory cytokine array kits (Ray Biotech Inc., GA, USA) following the manufacturer's instructions, as previously described [24]. The signal intensities were read by a chemiluminescence reader (Epi Chemi II Darkroom, UVP) and analyzed using the Ray Biotech cytokine expression analysis software.…”

Section: Determination Of Cytokines and Chemokines Secreted In The Pementioning

confidence: 99%

“…RNA quality was assessed using quality control plates (PAMM-999A-1, SA Biosciences, Frederick, MD). cDNA synthesis and expression analysis were performed using RT2 first-strand kit and RT2 Profiler PCR array (PAMM-097) respectively, from SA Biosciences, as previously described [24]. Gene expressions were normalized with respect to five house-keeping genes included in the array kit and expressed as averages of log 2 ratios.…”

Section: Real Time-pcr For Profiling Mrna Expression Of Inflammasome-mentioning

confidence: 99%

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Efficacy of phytol-derived diterpenoid immunoadjuvants over alum in shaping the murine host's immune response to Staphylococcus aureus

Chowdhury

Fitch

Ghosh

2013

Vaccine

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“…), phytanyl mannoside (2.5 and 5 mg/kg; i.p.) [69,70], phytanol (44 mg/kg, i.p.) and phytanyl chloride (44 mg/ kg; i.p) [12,71e78] have been proven to have potential immuneadjuvancy capacities in isogenic (mutant) albino mice.…”

Section: Pyt As Immune-adjuvancymentioning

confidence: 99%

Phytol in a pharma-medico-stance

Islam

Alencar

Machado

et al. 2015

Chemico-Biological Interactions

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Molecular signatures of phytol-derived immunostimulants in the context of chemokine–cytokine microenvironment and enhanced immune response

Cited by 16 publications

References 43 publications

Extracellular Matrix from Porcine Small Intestinal Submucosa (SIS) as Immune Adjuvants

Extracellular Matrix from Porcine Small Intestinal Submucosa (SIS) as Immune Adjuvants

Efficacy of phytol-derived diterpenoid immunoadjuvants over alum in shaping the murine host's immune response to Staphylococcus aureus

Phytol in a pharma-medico-stance

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