2014
DOI: 10.1016/j.dci.2013.09.017
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Abstract: Infectious diseases represent the most serious threat to shrimp farming worldwide. Understanding the molecular mechanisms driving shrimp-pathogen interactions is necessary for developing strategies to control disease outbreaks in shrimp production systems. In the current study, we experimentally reproduced mortality events using standardized infections to characterize the hemocyte transcriptome response of the shrimp Litopenaeus vannamei succumbing to infectious diseases. By using a high-throughput microfluidi… Show more

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Cited by 31 publications
(23 citation statements)
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“…The viral inoculum was prepared as previously described in [ 25 ], with some modifications. In brief, the abdomen tissues of WSSV-infected shrimp were homogenized in a Tris-saline solution (20 mM Tris, 330 mM NaCl, pH 7.4) (1:10 w/v ) using a hand blender.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The viral inoculum was prepared as previously described in [ 25 ], with some modifications. In brief, the abdomen tissues of WSSV-infected shrimp were homogenized in a Tris-saline solution (20 mM Tris, 330 mM NaCl, pH 7.4) (1:10 w/v ) using a hand blender.…”
Section: Methodsmentioning
confidence: 99%
“…In brief, the abdomen tissues of WSSV-infected shrimp were homogenized in a Tris-saline solution (20 mM Tris, 330 mM NaCl, pH 7.4) (1:10 w/v ) using a hand blender. The tissue homogenate was centrifuged twice (2000× g for 20 min and 9000× g for 10 min) at 4 °C and the supernatant filtered (0.45 µm), aliquoted and stored at −80 °C [ 25 ]. Eighty shrimp from each condition were challenged with WSSV inoculum (100 µL containing 5 × 10 6 viral copies) that was gently flowed into the oral cavity by using a micropipette.…”
Section: Methodsmentioning
confidence: 99%
“…In the shrimp L. vannamei, an infection by the fungal pathogen Fusarium solani dramatically repressed the expression of penaeidins, stylicins and Type II crustin [98]. Similarly, immune suppression of AMPs was reported in the coral P. damicornis, in which V. coralliilyticus exhibits temperaturedependent virulence, resulting in coral bleaching above 248C and tissue lysis above 258C [7].…”
Section: (B) Responses Of Pathogens To Amps (I) Immune Suppression Ofmentioning
confidence: 97%
“…The second line is based on cellular and humoral effectors, which combine to combat pathogens, heal wounds and respond to biotic and abiotic stressors (Lemaitre and Hoffman, 2007). The cellular effectors in shrimp are hemocytes (granular, semigranular, and hyaline), which play a central role in phagocytosis, melanization, encapsulation, nodulation, cytotoxicity, apoptosis, interfering RNA, autophagy, interferon-type system, and coagulation of the hemolymph (Goncalves et al, 2014;Sun et al, 2010). The humoral effectors are the second line of defense and include agglutinins, profenoloxidase system, coagulation proteins, hydrolytic enzymes, antimicrobial peptides, nitric oxide, respiratory burst, and opsonization (Morales and Cuéllar et al, 2014;Cerenius and Söderhäll, 2004;Destoumieux et al, 2000;Jiravanichpaisal et al, 2006).…”
Section: Introductionmentioning
confidence: 99%