Molecular regulation of blastocyst formation

Watson, Andrew J.; Natale, David R.C.; Barcroft, Lisa C.

doi:10.1016/j.anireprosci.2004.04.004

Cited by 100 publications

(27 citation statements)

References 55 publications

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“…Blastocyst development is regulated by the expression of specific gene families that encode for cell polarity, cell junctional, cytoskeletal, ion transporter, and water channel gene products (10), and the degree of expansion of the human blastocyst is positively associated with pregnancy and implantation rates (11,12). Together, these observations suggest that culture in Global medium is more favorable for transcriptional activity and functional potential of the embryo compared with culture in ECM/Multiblast.…”

Section: Discussionmentioning

confidence: 92%

In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system

Sepúlveda¹,

García²,

Arriaga³

et al. 2009

Fertility and Sterility

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Section: Discussionmentioning

confidence: 92%

In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system

Sepúlveda¹,

García²,

Arriaga³

et al. 2009

Fertility and Sterility

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“…The trophectoderm, which is the first layer of epithelium formed during embryonic development, initiates contact with the endometrium and promotes embryonic invasion into the endometrium during implantation (Watson et al 2004). Interestingly, this process involves EMT in order to facilitate invasion into the endometrium (Kalluri and Weinberg 2009).…”

Section: Snail and Slug Expression During Early Embryonic Cleavagementioning

confidence: 99%

Expression and localization of transcription factors SNAIL and SLUG in mouse ovaries and pre-implantation embryos

et al. 2014

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SNAIL and SLUG are zinc-finger transcription factors that participate in the regulation of cell division, cell survival, mesoderm formation and epithelial-to-mesenchymal transition. We investigate the expression of SNAIL and SLUG during follicular maturation, ovulation and luteinization in the ovaries of both neonatal mice and gonadotropin-induced immature mice. Furthermore, we examine the expression and localization of these transcription factors during early embryonic cleavage. Our data demonstrate that both SNAIL and SLUG are present in the epithelial cells of the ovarian surface in immature mice. SNAIL is first evident in the interstitial cells and theca cells by postnatal day (PD) 6 and then appears in the oocytes by PD 8, remaining at a constant expression level for all stages studied thereafter. SLUG is expressed in oocytes as early as PD 1. Its expression also increases with the development of the follicles in theca and interstitial cells but not in granulosa cells. In gonadotropin-induced immature mice, both SNAIL and SLUG are expressed in the corpora lutea. During early embryo cleavage, SNAIL occurs in the nucleus and cytoplasm of the majority of the embryo, excluding the nucleolus from the germinal vesicle breakdown (GVBD) to the 8-cell stage and is then localized in the cytoplasm during the morula stage and in the nucleus during the blastocyst stage. SLUG has an identical expression pattern as SNAIL from GVBD until the morula stage, except that it is localized in the cytoplasm during the blastocyst stage. Taken together, these different localization patterns suggest that SNAIL and SLUG probably play important roles during follicular development, luteinization and early embryonic development.

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“…The reference standard for these experiments was conventional supplementation with hormones (FSH, LH, and E 2 ) and a serum-derived protein source, in our case KOSR (Invitrogen), used routinely for human embryo stem cell culture (36). Supplementation with hormones and fetal calf or human serum has been used extensively by us and other investigators in bovine and human IVM to yield oocytes with demonstrated competence for parthenogenetic and fertilized embryo development (17,37,38). After confirming that KOSR was as good or superior to fetal calf serum in bovine IVM experiments (data not shown), we elected to use it in human IVM to reduce the potential for batch variations associated with reliance on serum.…”

Section: Human Oocyte Maturation In Vitro With Bdnf and Antagonistsmentioning

confidence: 99%

Brain-derived neurotrophic factor is a regulator of human oocyte maturation and early embryo development

Anderson

Bayne

Gardner

et al. 2010

Fertility and Sterility

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Molecular regulation of blastocyst formation

Cited by 100 publications

References 55 publications

In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system

In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system

Expression and localization of transcription factors SNAIL and SLUG in mouse ovaries and pre-implantation embryos

Brain-derived neurotrophic factor is a regulator of human oocyte maturation and early embryo development

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