Molecular phylogeny of Diabrotica beetles (Coleoptera: Chrysomelidae) inferred from analysis of combined mitochondrial and nuclear DNA sequences

Abstract

Meinke, Lance J.; and Foster, John E., "Molecular phylogeny of Diabrotica beetles (Coleoptera: Chrysomelidae) inferred from analysis of combined mitochondrial and nuclear DNA sequences" (2001). Faculty Publications: Department of Entomology. 633. http://digitalcommons.unl.edu/entomologyfacpub/633 AbstractThe phylogenetic relationships of thirteen Diabrotica (representing virgifera and fucata species groups) and two outgroup Acalymma beetle species (Coleoptera: Chrysomelidae) were inferred from the phylogenetic analysis of a combined data set of 1323 bp of mitochondrial DNA (mtDNA) cytochrome oxidase subunit 1 (COI) and the entire second internal transcribed spacer region (ITS-2) of nuclear ribosomal DNA of 362 characters. Species investigated were D. adelpha, D. balteata, D. barberi, D. cristata, D. lemniscata, D. longicornis, D. porracea, D. speciosa, D. undecimpunctata howardi, D. u. undecimpunctata, D. virgifera virgifera, D. v. zeae, D. viridula, and outgroup A. blandulum and A. vittatum. Maximum parsimony (MP), minimum evolution (ME), and maximum likelihood (ML) analyses of combined COI and ITS-2 sequences clearly place species into their traditional morphological species groups with MP and ME analyses resulting in identical topologies. Results generally confer with a prior work based on allozyme data, but within the virgifera species group, D. barberi and D. longicornis strongly resolve as sister taxa as well as monophyletic with the neotropical species, D. viridula, D. cristata and D. lemniscata also resolve as sister taxa. Both relationships are not in congruence with the prior allozyme-based hypothesis. Within the fucata species group, D. speciosa and D. balteata resolve as sister taxa. Results also strongly supported the D. virgifera and D. undecimpunctata subspecies complexes. Our proposed phylogeny provides some insight into current hypotheses regarding distribution status and evolution of various life history traits for Diabrotica.

Effect of Cry3Bb1-Expressing Transgenic Corn on Plant-to-Plant Movement by Western Corn Rootworm Larvae (Coleoptera: Chrysomelidae)

“…Following the methods of Clark et al (2001a), a 625-bp portion of COI was ampliÞed using the universal primers from the COI (C1-J-2441 5Ј-CCAACAGGAATTAAAATTTTTAGA-TGATTAGC-3Ј) and the tRNA leucine genes (TL2-N-3014 5Ј-TCCAATGCACTAATCTGCCATATTA-3Ј) (Simon et al 1994) by using a GeneAmp PCR system 2700 (PerkinElmer, Branchburg, NJ). PCR ampliÞcation products (2 l) were loaded onto 1.0% agarose TBE (0.089 M Tris, 0.089 M boric acid, and 0.5 M EDTA, pH 8.0) gels.…”

Comparison of Nonmaize Hosts to Support Western Corn Rootworm (Coleoptera: Chrysomelidae) Larval Biology

“…THE GENUS Diabrotica is divided into three species groups (virgifera, fucata, and signifera) that can be distinguished by morphology (Wilcox 1972, Krysan andSmith 1987), mitochondrial and nuclear DNA genes (Clark et al 2001), and biology and life history traits. One of the more intriguing distinctions between the groups is larval host selection.…”

Preliminary phylogeny of Tribolium beetles (Coleoptera: Tenebrionidae) resolved by combined analysis of mitochondrial genes

“…Future work on this genus should therefore include additional molecular markers, preferably some of the nuclear genes or genomic regions that proved useful for investigations of sub-familial interrelationships of insects, such as ribosomal RNA genes, elongation factors or ITS regions (e.g. Danforth et al, 2005;Gómez-Zurita & Galián, 2005;Gómez-Zurita et al, 2005;Becerra, 2004;Whitfield et al, 2002;Rokas et al, 2002;Cryan et al, 2001;Clark et al, 2001;Krzywinski et al, 2001;Mardulyn & Whitfield, 1999).…”