Molecular phylogeny in Indian Citrus L. (Rutaceae) inferred through PCR-RFLP and trnL-trnF sequence data of chloroplast DNA

Jena, Satya Narayan; Kumar, Susheel; Nair, Narayanan K.

doi:10.1016/j.scienta.2008.08.030

Cited by 54 publications

(36 citation statements)

References 42 publications

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“…The PCR-RFLP approach to study organellar genome diversity has been reported in many plants (Tsumura et al, 1995(Tsumura et al, , 1996Lakshmi et al, 2000;Parani et al, 2000Parani et al, , 2001Komatsu et al, 2001;Kishimoto et al, 2003;Nwakanma et al, 2003;Zhu et al, 2003;Asadi Abkenar et al, 2004Van Droogenbroeck et al, 2004;Ibrahim et al, 2007;Sehgal et al, 2008;Jena et al, 2009;Poczai et al, 2011). This work aimed at deciphering the polymorphism in the chloroplast and mitochondrial genomes in the Saccharum spp.…”

Section: Discussionmentioning

confidence: 99%

“…Specific chloroplast genes and/or intergenic spacers can be amplified (Taberlet et al, 1991;Demesure et al, 1995;Tsumura et al, 1995Tsumura et al, , 1996Dhingra and Folta, 2005;Heinze, 2007). The amplicons can be directly sequenced or restriction endonuclease digested (PCR-RFLP) or subjected to cleaved amplified polymorphic sequence (Tsumura et al, 1995(Tsumura et al, , 1996Lakshmi et al, 2000;Parani et al, 2000Parani et al, , 2001Komatsu et al, 2001;Kishimoto et al, 2003;Nwakanma et al, 2003;Zhu et al, 2003;Asadi Abkenar et al, 2004Van Droogenbroeck et al, 2004;Ibrahim et al, 2007;Sehgal et al, 2008;Jena et al, 2009;Poczai et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Organellar Genome Diversity in Saccharum and Erianthus spp. revealed by PCR-RFLP

Nerkar¹,

Farsangi²,

Devarumath³

2015

mpb

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The organellar genome diversity in Saccharum and Erianthus species was analysed by chloroplast deoxyribonucleic acid (cpDNA) and mitochondrial deoxyribonucleic acid (mtDNA) polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Six different chloroplast primers trnL, psaA, clpP, matK and ccsA); and ten mitochondrial primers (nad1, coxI, matR, cob and mttb) were used to amplify the genes/intergenic spacers of 8 different members of Saccharum complex namely S. officinarum, S. robustum, S. spontaneum, S. barberi, E. arundinaceus, E. ciliaris, E. elegans and CoC 671 (S. officinarum hybrid). The amplified PCR-products were digested with ten different restriction enzymes namely AluI, BamHI, BglII, DraI, EcoRI, HaeIII, HindIII, HinfI and PstI, TaqI. Our results suggest that although monomorphic bands were observed with the PCR using chloroplast and mitochondrial primers; there exists restriction fragment length polymorphism in these genes/intergenic spacers. Out of the sixty primer-enzyme combinations studied, thirty primer-enzyme combinations revealed cpPCR-RFLP while out of hundred primer-enzyme combinations studied fifty-seven primer-enzyme combinations revealed mtPCR-RFLP in sugarcane. Differentiation in Saccharum and Erianthus species was found in the psbC-trnS region of the chloroplast digested using enzyme HaeIII and also in the trnL region digested using enzyme TaqI. One new finding in our work was the mtPCR-RFLP revealed by nad4/2-3 region restriction digested using enzyme AluI which was able to differentiate Saccharum species and Erianthus species. Our results may add to the knowledge about organellar genome diversity in sugarcane and may be useful for identification of sugarcane hybrids.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Organellar Genome Diversity in Saccharum and Erianthus spp. revealed by PCR-RFLP

Nerkar¹,

Farsangi²,

Devarumath³

2015

mpb

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“…The citron (Citrus medica L.) belongs to the family Rutaceae, subfamily Aurantiaca, tribe Citrae (1). Systematic studies highlight the major role of citron in the phylogeny of several varieties of the Citrus genus as the pollinator parent (2)(3)(4)(5)(6). All taxonomists recognize the contribution of the citron to the development of several important cultivated genotypes.…”

Section: Introductionmentioning

confidence: 99%

Volatile and Flavonoid Composition of the Peel of Citrus medica L. var. Corsican Fruit for Quality Assessment of Its Liqueur

Venturini¹,

Barboni²,

Curk³

et al. 2014

Food. Technol. Biotechnol.

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SummaryThe volatile and fl avonoid compositions of the peel of Citrus medica L. var. Corsican fruits cultivated in Corsica were studied according to the maturity of the citron fruits measured using growing degree-days. Quantitative variation with the stage of development of the fruit was observed using gas chromatography, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry/mass spectrometry. Thirty volatile compounds were identifi ed in the peel essential oil. Limonene and γ-terpinene were the major compounds. The volatile compositions of commercial citron liqueurs were also characterized by high amounts of monoterpene hydrocarbons with the same two major components. The main fl avonoid components of citron fruits and derived liqueurs were rutin and neohesperidin. This chemical characterization can be used for quality assessment of food products from C. medica var. Corsican.

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“…Furthermore, the same authors (EL-Mouei et al, 2011b) found that among the four Citrus groups, Lemon is distant from the others (Mandarins, Grapefruits and Sweet orange) and the highest genetic diversity was detected in the Mandarin and the lowest in the Grapefruit group. Several molecular studies supported C. maxima, Citrus medica and Citrus reticulata as the basal species of edible Citrus and identified probable hybrid origins of several commercial cultivars (Jena et al, 2009;Pessina et al, 2011;Ramadugu et al, 2013). In an attempt to identify the paternal and maternal origins of 30 accessions of cultivated Citrus, Li and Xie (2010) has employed three marker systems; the chloroplast DNA and internal transcribed spacer sequences and AFLP fingerprints.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of citrus (Rutaceae) in Iraq based on random amplified polymorphic DNA (RAPD) markers

Al-Anbari¹,

Kanawapee²,

AL-Kazragi³

et al. 2014

Afr. J. Agric. Res.

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Citrus is an economically important fruit crop with a long history of cultivation worldwide. A great number of varieties of citrus are extensively grown in the Middle East including Iraq for domestic consumption and exports. However, the genetic diversity of this genus in Iraq has not been reported. Therefore, the objective of this study was to evaluate genetic relationships of Iragi citrus genotypes to provide useful information for germplasm conservation and planning of breeding strategies. Twenty decamer primers were used to generate RAPD markers to evaluate genetic relationship among 16 genotypes (14 species and hybrids) of cultivated Citrus in Iraq. Based on RAPD polymorphisms, the citrus genotypes were classified into two main groups; the first consisted of citron (Citrus medica) and its hybrids (lime and lemon). The second group contained the remaining genotypes including three subgroups; the first being formed of sour orange (Citrus aurantium), sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi), the second the mandarins (Citrus reticulata) and the third the pummelo (Citrus grandis). The RAPD-based classification was consistent with previous studies based on other types of molecular markers.

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Molecular phylogeny in Indian Citrus L. (Rutaceae) inferred through PCR-RFLP and trnL-trnF sequence data of chloroplast DNA

Cited by 54 publications

References 42 publications

Organellar Genome Diversity in <i>Saccharum</i> and <i>Erianthus</i> spp. revealed by PCR-RFLP

Organellar Genome Diversity in <i>Saccharum</i> and <i>Erianthus</i> spp. revealed by PCR-RFLP

Volatile and Flavonoid Composition of the Peel of Citrus medica L. var. Corsican Fruit for Quality Assessment of Its Liqueur

Genetic diversity of citrus (Rutaceae) in Iraq based on random amplified polymorphic DNA (RAPD) markers

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