Molecular phylogeny and evolutionary history of the Eurasiatic orchid genus Himantoglossum s.l. (Orchidaceae)

Sramkó, Gábor; Attila, Molnár V.; Hawkins, Julie A.; Bateman, Richard M.

doi:10.1093/aob/mcu179

Cited by 46 publications

(95 citation statements)

References 68 publications

(85 reference statements)

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“…(i.e. including the former genera Comperia and Barlia) derived by Bateman et al (2003), and placed the Caucasian endemic H. formosum in the predicted position as sister to the remaining taxa of Himantoglossum s.s. (Sramkó et al, 2013). However, no reliable phylogenetic structure was identified within this residual clade, which identified only single autapomorphic states in ITS supporting the putative eastern Mediterranean endemics H. montis-tauri and H. galilaeum (Fig.…”

Section: Its Ribotypesmentioning

confidence: 88%

“…Experimental details of molecular data acquisition were given by Sramkó et al (2013). Here, we focus on the preceding sampling strategy and succeeding data analysis.…”

Section: Molecular Analysesmentioning

confidence: 99%

“…Direct sequencing of ITS (performed in Korea by Macrogen) in a total of 46 plants revealed frequent individuals that showed additive polymorphic sites in ITS. Hence, one sample from three selected populations of H. (Sramkó et al, 2013). Most variable sites were single nucleotide polymorphisms, but ITS 'populations' within five plants were length polymorphic (i.e.…”

Section: Molecular Analysesmentioning

confidence: 99%

“…Further molecular investigations, conducted as part of a broader phylogenetic study of the genus Himantoglossum sensu lato (Sramkó et al, 2013), included five samples of H. hircinum that were selected to span the full geographic distribution of the species: Newmarket (England) to the northwest, Habkirchen (SW Germany) to the northeast, Grasse (SE France) representing the core distribution, Pacios (NW Spain) representing the northern Iberian outlier, and Calvello (S Italy) representing the southern Italian outlier. These samples were sequenced for the first intron of the low-copy (and developmentally crucial) nuclear gene LEAFY and for three rapidly mutating and phylogenetically informative plastid regions: the accD-psaI intron, the psbA-trnH intron, and two introns of the trnL-ndhF region, specifically trnL-rpl32 and rpl32-ndhF.…”

Section: Molecular Analysesmentioning

confidence: 99%

“…Delforge, 1999;Sramkó et al, 2013), the process has been less rigorous than in several other genera of European Orchideae (cf. Bateman, 2013).…”

mentioning

confidence: 99%

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Himantoglossum hircinum(Lizard Orchid) reviewed in the light of new morphological and molecular observations

Bateman

Rudall

Hawkins

et al. 2013

New Journal of Botany

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[Running Head: Bateman et al. Observations on Lizard Orchid populations]Himantoglossum hircinum is one of the rarer and more charismatic orchids in the British flora. Morphometric comparison of the two largest and best-known populations in southern England -the coastal dune population at Sandwich and the chalk grassland population at Newmarket -using 46 characters showed that they differ only subtly, the Sandwich plants being on average more vegetatively robust and slightly more darkly pigmented, but possessing less extensive lip-spots and substantially longer 'arms'. A comparatively morphologically divergent semi-desert population from Ifrane, Morocco differs from the English populations in having broader stems, less recurved 'arms', a more strongly down-curved spur and in lacking near-circular spots within the sepals. Molecular comparison of 46 plants, representing 13 English populations and 18 populations from Continental Europe and Morocco, revealed only subtle distinctions in the high-copy nuclear gene ITS, and smaller-scale comparisons of the low-copy nuclear (LEAFY) and plastid (four intron) regions proved to be even less discriminatory. These results reinforce prior morphological inferences that H. hircinum is a cohesive species. Scanning electron microscopy elucidated the ontogeny of these remarkable flowers, suggesting that the exceptionally elongate central labellar lobe originated by accelerated heterochronic growth and showing that the characteristic spiral torsion always runs counter-clockwise. Lateral fusion of the paired viscidia is convergent with several other lineages of subtribe Orchidinae. Review of pollination and life-history features of H. hircinum suggest that they are typical of food-deceptive species within Orchidinae. The Lizard Orchid is infamous for geographic mobility; its cycles of expansion and contraction through the last century have been interpreted as reflecting a net northward migration in response to recent climate change. Our data tentatively suggest relatively recent colonisation of Morocco at high altitudes and an overall northwestward direction of migration into the UK. ITS ribotypes indicate multiple immigration events leading to levels of genetic diversity in England comparable with those on the Continent. A nonrecent origin is inferred for H. hircinum which, despite recent systematic revisions, may *Manuscript Click here to download Manuscript: Hircinum_NJB_Text_v8. doc 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 harbour further cryptic species; the taxonomic status of supposed outlying populations in southern Italy in particular is questioned by the present genetic data.

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Section: Its Ribotypesmentioning

confidence: 88%

“…Experimental details of molecular data acquisition were given by Sramkó et al (2013). Here, we focus on the preceding sampling strategy and succeeding data analysis.…”

Section: Molecular Analysesmentioning

confidence: 99%

Section: Molecular Analysesmentioning

confidence: 99%

Section: Molecular Analysesmentioning

confidence: 99%

“…Delforge, 1999;Sramkó et al, 2013), the process has been less rigorous than in several other genera of European Orchideae (cf. Bateman, 2013).…”

mentioning

confidence: 99%

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Himantoglossum hircinum(Lizard Orchid) reviewed in the light of new morphological and molecular observations

Bateman

Rudall

Hawkins

et al. 2013

New Journal of Botany

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Hundreds of nuclear and plastid loci yield novel insights into orchid relationships

Pérez‐Escobar

Dodsworth

Bogarín

et al. 2021

American J of Botany

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The inference of evolutionary relationships in the species-rich family Orchidaceae has hitherto relied heavily on plastid DNA sequences and limited taxon sampling. Previous studies have provided a robust plastid phylogenetic framework, which was used to classify orchids and investigate the drivers of orchid diversification. However, the extent to which phylogenetic inference based on the plastid genome is congruent with the nuclear genome has been only poorly assessed. METHODS:We inferred higher-level phylogenetic relationships of orchids based on likelihood and ASTRAL analyses of 294 low-copy nuclear genes sequenced using the Angiosperms353 universal probe set for 75 species (representing 69 genera, 16 tribes, 24 subtribes) and a concatenated analysis of 78 plastid genes for 264 species (117 genera, 18 tribes, 28 subtribes). We compared phylogenetic informativeness and support for the nuclear and plastid phylogenetic hypotheses.RESULTS: Phylogenetic inference using nuclear data sets provides well-supported orchid relationships that are highly congruent between analyses. Comparisons of nuclear gene trees and a plastid supermatrix tree showed that the trees are mostly congruent, but revealed instances of strongly supported phylogenetic incongruence in both shallow and deep time. The phylogenetic informativeness of individual Angiosperms353 genes is in general better than that of most plastid genes. CONCLUSIONS:Our study provides the first robust nuclear phylogenomic framework for Orchidaceae and an assessment of intragenomic nuclear discordance, plastid-nuclear tree incongruence, and phylogenetic informativeness across the family. Our results also demonstrate what has long been known but rarely thoroughly documented: nuclear and plastid phylogenetic trees can contain strongly supported discordances, and this incongruence must be reconciled prior to interpretation in evolutionary studies, such as taxonomy, biogeography, and character evolution.

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Development and characterization of novel SSR markers in the endangered endemic species Ferula sadleriana

et al. 2020

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Premise Ferula sadleriana (Apiaceae) is a polycarpic, perennial herb with a very limited range and small populations. It is listed as “endangered” on the IUCN Red List of Threatened Species. Microsatellite markers can contribute to conservation efforts by allowing the study of the genetic structure of its shrinking populations. Methods and Results We used a microsatellite‐enriched library combined with an Illumina sequencing approach to develop simple sequence repeat markers in our target species. Out of 44 tested primer pairs, 22 provided specific products, and 13 showed heterologous amplification in the target species. Cross‐species amplification was achieved at 20 and 19 loci in two congeneric species, F. soongarica and F. tatarica, respectively. Conclusions The primers described here are the first tools that enable the population genetic characterization of F. sadleriana. Our results suggest a wider applicability in the genus Ferula.

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Molecular phylogeny and evolutionary history of the Eurasiatic orchid genus Himantoglossum s.l. (Orchidaceae)

Cited by 46 publications

References 68 publications

Himantoglossum hircinum(Lizard Orchid) reviewed in the light of new morphological and molecular observations

Himantoglossum hircinum(Lizard Orchid) reviewed in the light of new morphological and molecular observations

Hundreds of nuclear and plastid loci yield novel insights into orchid relationships

Development and characterization of novel SSR markers in the endangered endemic species Ferula sadleriana

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