Molecular Organization of the Distal Cilium, a Potential Mechanotransduction Site, in Campaniform Receptor of Drosophila Melanogaster

Liang, Xin; Madrid, Johnson; Saleh, Henri S.; Howard, Jonathon

doi:10.1016/j.bpj.2010.12.1742

Cited by 1 publication

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“…Second, NOMPC is distinct to the other TRP channels by a special structural feature, its N-terminal ankyrin repeats. In NOMPC 29 ankyrins form one turm of a functional spring (Howard et al, 2004;Liang et al, 2011) . The predicted gating spring stiffness of hair cell transduction channels matches the stiffness of this spring (Howard et al, 2004;Lee et al, 2006;Sotomayor et al, 2005) .…”

Section: Auditory Transduction In Drosophilamentioning

confidence: 99%

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A Functional Characterisation of Drosophila Chordotonal Organs

Wiek¹

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A constant challenge for the insect's nervous system is the omnipresence of diverse stimuli. These external cues trigger the organisms behavioural patterns to alternating environmental conditions. Therefore insects are endowed with specialized sense organs. These sensory neurons respond adequately to specific stimulation, as specific behaviour is only evoked by specific sensory stimuli.The basis for specific behavioural patterns of insects is modulated by the senses of photoreception, proprioreception, chemoreception, thermosensation, touch and hearing. In Drosophila, specific sounds entail courtship (Spieth et al., 1952, Shorey et al., 1962, Narda et al., 1966 . It is ascertained that Drosophila does hear with its antennae via scolopidia, which are arranged in a chordotonal organ called Johnston´s Organ (JO) (Johnston 1855; Boekhoff-Falk G et al., 2005; Yack et al., 2004) . The JO in the antenna is the largest chordotonal organ in Drosophila followed by the femoral chordotonal organ (FCO) in the leg of Drosophila. Insect legs are endowed with mechanoreceptors, the femoral chordotonal organs (FCOs) (Göpfert et al., 2005;Nishino et al., 2003; Field et al., 1998; Büschges et al., 1994) , that detect angular changes between the femur and the tibia. In Drosophila, little is known about the function of the FCO (Shanbhag et al., 1992) .Here I show that functional properties of the Drosophila FCO can be dissected by use of the Gal4-UAS system in combination with transcuticular in vivo Calcium imaging. As sinusoidal stimulation at relative small stimulus amplitudes elicited changes in intracellular ionic calcium (Ca 2+ ) concentration in the FCO, the effect of substrate vibration on Drosophila walking behaviour was proposed. But lately developed fly tracking software for high-throughput ethomics (Branson et al., 2009) showed that substrate vibrations have no specific effect on Drosophila walking behaviour. Instead present evidence suggests that Drosophila compensates for substrate vibration by detection via the FCO and therefore is able to control its body posture. A modification of 1 | Introduction 1.2 | Sensory Neurons of Insects 1.2.1 | External Sense Organs transcuticular in vivo Calcium imaging was used to locate the neuronal activity region of Inactive, a protein involved in Drosophila hearing and a mechanotransduction channel candidate. I could show that inactive mutants still respond to sound like stimulation with ciliary calcium currents. | Sensory Neurons of InsectsThe peripheral nervous system of insects comprises two major classes of sensory organs: Type I neurons are monodendritic and ciliated, bearing a single ciliated dendrite (Tracey et al., 2003) , whereas type II neurons are multidendritic neurons (MD) whose dendrites form no ciliar structures (Keil et al., 1997) .Type I organs are further subclassified into photoreceptors, external sensory organs (ES) (involving gustatoric, olfactoric neurons and mechanoreceptive sensilla like bristle and campaniform sensilla) and mechanosensitive chordoton...

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Section: Auditory Transduction In Drosophilamentioning

confidence: 99%

“…This model is in consistency with the localisation of the afore mentioned channels. In the JO NOMPC is located at the most distal part of the scolopidial cilium that is directly attached to the sound receiver (Cheng et al, 2010 ;Lee et al, 2010;Liang et al, 2011)…”

Section: Auditory Transduction In Drosophilamentioning

confidence: 99%

A Functional Characterisation of Drosophila Chordotonal Organs

Wiek¹

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Molecular Organization of the Distal Cilium, a Potential Mechanotransduction Site, in Campaniform Receptor of Drosophila Melanogaster

Cited by 1 publication

References 0 publications

A Functional Characterisation of Drosophila Chordotonal Organs

A Functional Characterisation of Drosophila Chordotonal Organs

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