Molecular Microfluidic Bioanalysis: Recent Progress in Preconcentration, Separation, and Detection

Abstract

This chapter reviews the state-of-art of microfluidic devices for molecular bioanalysis with a focus on the key functionalities that have to be successfully integrated, such as preconcentration, separation, signal amplification, and detection. The first part focuses on both passive and electrophoretic separation/sorting methods, whereas the second part is devoted to miniaturized biosensors that are integrated in the last stage of the fluidic device.

Micropatterning in BioMEMS for Separation of Cells/Bioparticles

“…Nonetheless in recent years Klebsiella species has now become one of the important antibody in infections like nosocomial even there are some important members of Klebsiella breed of Entero-bacteriaceae and some have been exhibited in human laboratory specimens like K. rhinoscleromatis and K. oxytoca [7]. However separation and detection for the manipulations from the fluidic sample of bioparticles using microfluidics are sensible and challenging issues in laboratories depending on the flow rates, throughput and clogging on microscale [5].…”

“…First part of this chapter deals with the active and passive approaches for bioparticles and cellular separation depending on the fluid velocities and its concentration depending on applications and designing. The second half of the chapter focuses on the simulations of the sorting and the detection techniques [5].…”