Molecular mechanisms of luteolin-7-O-glucoside-induced growth inhibition on human liver cancer cells: G2/M cell cycle arrest and caspase-independent apoptotic signaling pathways

Abstract

Luteolin-7-O-glucoside (LUT7G), a flavone subclass of flavonoids, has been found to increase anti-oxidant and anti-inflammatory activity, as well as cytotoxic effects. However, the mechanism of how LUT7G induces apoptosis and regulates cell cycles remains poorly understood. In this study, we examined the effects of LUT7G on the growth inhibition of tumors, cell cycle arrest, induction of ROS generation, and the involved signaling pathway in human hepatocarcinoma HepG2 cells. The proliferation of HepG2 cells was decreased by LUT7G in a dose-dependent manner. The growth inhibition was due primarily to the G2/M phase arrest and ROS generation. Moreover, the phosphorylation of JNK was increased by LUT7G. These results suggest that the anti-proliferative effect of LUT7G on HepG2 is associated with G2/M phase cell cycle arrest by JNK activation. [BMB Reports 2013; 46(12): 611-616]

Luteoloside attenuates anoxia/reoxygenation‐induced cardiomyocytes injury via mitochondrial pathway mediated by 14‐3‐3η protein

“…Many studies have shown that flavonoids may protect cardiomyocytes against I/R injury (Yuan et al, ; Zhang et al, ). Lut, a flavonoid compound extracted from honeysuckle, exhibits anti‐inflammatory, antiviral, and anticancer properties (Cao et al, ; Hwang, Lee, Kim, & Hwang, ; Jung, Jin, Min, Kim, & Choi, ; Tian et al, ) and has shown promising results in myocardial cells. A/R injury was used to model I/R injury in vitro.…”

Tat-biliverdin reductase A inhibits inflammatory response by regulation of MAPK and NF-κB pathways in Raw 264.7 cells and edema mouse model

“…Measurement of intracellular ROS levels 2 ,7 -dichlorofluorescein diacetate (DCF-DA) staining was used to determine intracellular ROS levels, we performed as described previously (Ahn et al, 2013;Hwang et al, 2013). After Raw 264.7 cells were treated with Tat-BLVRA protein, the cells were treated with LPS (1 g) for 30 min.…”

HIF‐1α/VEGF signaling‐mediated epithelial–mesenchymal transition and angiogenesis is critically involved in anti‐metastasis effect of luteolin in melanoma cells

“…Previous studies indicate that luteolin suppress cancer cell growth through multiple mechanisms including promoting cancer cell apoptosis and cell cycle arrest, inhibiting cell proliferation and tumor growth, improving drug resistance, and mitigating invasiveness and metastasis of cancer cells (Cook, Liang, Besch-Williford, & Hyder, 2017;Wang et al, 2014). Specifically, it has been found that luteolin inhibited the tumor formation in vivo through its antioxidant and anti-inflammatory activity (Hwang, Lee, Kim, & Hwang, 2013). Furthermore, luteolin effectively blocked progestin-dependent angiogenesis and the stem cell-like phenotype in human breast cancer cells (Cook, 2018).…”