Molecular Mechanisms of Brassinosteroid-Mediated Responses to Changing Environments in Arabidopsis

Abstract

Plant adaptations to changing environments rely on integrating external stimuli into internal responses. Brassinosteroids (BRs), a group of growth-promoting phytohormones, have been reported to act as signal molecules mediating these processes. BRs are perceived by cell surface receptor complex including receptor BRI1 and coreceptor BAK1, which subsequently triggers a signaling cascade that leads to inhibition of BIN2 and activation of BES1/BZR1 transcription factors. BES1/BZR1 can directly regulate the expression of thousands of downstream responsive genes. Recent studies in the model plant Arabidopsis demonstrated that BR biosynthesis and signal transduction, especially the regulatory components BIN2 and BES1/BZR1, are finely tuned by various environmental cues. Here, we summarize these research updates and give a comprehensive review of how BR biosynthesis and signaling are modulated by changing environments and how these changes regulate plant adaptive growth or stress tolerance.Int. J. Mol. Sci. 2020, 21, 2737 3 of 20 BR signal cascade by phosphorylating BR SIGNALING KINASE 1 (BSK1) and CDG1, two receptor-like cytoplasmic kinases (RLCKs) that are anchored to the cell membrane [59,60]. Activated CDG1 can subsequently phosphorylate and activate a protein phosphatase BSU1, which then dephosphorylates and inactivates a GSK3 kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) [60][61][62]. BIN2 is a negative regulator in the BR signaling pathway because it phosphorylates and destabilizes two well-characterized downstream transcription factors, BRI1-EMS-SUPPRESSOR 1 (BES1) and BRSSINAZOLE-RESISTANT 1 (BZR1), and presumably other four members of their entire subfamily [63][64][65][66][67]. BR-induced BIN2 inactivation allows the accumulation of nonphosphorylated BES1 and BZR1 in the nucleus, thereby promoting expression of thousands of their target genes [62,68]. The intensity of BR signaling can be controlled at multiple levels (Figure 2). At the receptor level, the function of BRI1 can be post-translationally regulated by PUB12/13-directed ubiquitination, PP2A-mediated dephosphorylation, BKI1-mediated kinase inhibition, and BIK1-and BIR3-mediated competition for the coreceptor BAK1 [69][70][71][72][73]. At the BIN2 level, it has been reported that BIN2 is regulated by OCTOPUSor POLAR-mediated membrane sequestration, HDA6-mediated deacetylation, KIB1-mediated ubiquitination, TTL-enhanced interaction with BSU1, ABI1/2-mediated dephosphorylation, and ROS (reactive oxygen species)-mediated oxidation [74][75][76][77][78][79][80]. At the transcription level, it has been reported that PP2A phosphatases can promote BR signaling by dephosphorylating BES1 and BZR1, whereas 14-3-3 and BRZ-SENSITIVE-SHORT HYPOCOTYL1 (BSS1) negatively regulate BR signaling by inhibiting the translocation of BES1 and BZR1 from the cytosol to the nucleus [81][82][83]. Besides, protein degradation of BES1 and/or BZR1 has been reported to be regulated by many factors, including an F-box protein MORE AXILLARY GROWTH LOCUS2 (MAX2), ubiquitin E3 ligases suc...

Interplay between Hormones and Several Abiotic Stress Conditions on Arabidopsis thaliana Primary Root Development

“…BR: The level of BRI1 protein decreases under high temperature whereas the level of the transcript does not change [255] and bri1 BR receptor mutant has longer PRs than WT plants in response to high temperature (26 • C-29 • C). Accordingly, the constitutive BR response mutants, bzr1-D and bes1-D, and the triple GSK (Glycogen Synthase Kinase) mutant bin2 sk2-2 (bil1) sk2-3 (bil2) (defective in the negative regulation of the BR pathway) have shorter PRs at 26 • C compared to WT plants ( Figure 5A and Table S2) [255,261].…”

Adaptive and maladaptive expression plasticity underlying herbicide resistance in an agricultural weed

“…We were able to annotate 25 of the 26 genes with evidence of maladaptive plasticity . Some of these genes showed connections to stress responses, such as a homolog of the gene BAK1 (AT4G33430.1) Lv and Li 2020) . However, most of the genes did not have clear connections to herbicide or other stress responses (Table S3).…”

Modes of Brassinosteroid Activity in Cold Stress Tolerance

“…The end products of BR biosynthesis castasterone (CS) and brassinolide (BL) are bioactive; they act in minute concentrations, which are closely monitored and adjusted. This is executed by regulation of genes involved in BR biosynthesis and catabolism, depending on the needs of intrinsic growth programs, but also according to requirements for growth adaptation and stress protection (reviewed in Clouse, 2011;Lv and Li, 2020). With respect to cold stress, there is evidence that the BR biosynthetic genes DWF4, CPD, and BR6ox2 are rapidly downregulated by cold treatment (Eremina et al, 2016b).…”