Molecular markers linked to papaya ring spot virus resistance and Fusarium race 2 resistance in melon

Abstract

In melon, the Fom-1 gene confers monogenic resistance against the soil-borne fungus Fusarium oxysporum f. sp. melonis, races 0 and 2, while the closely linked Prv gene specifies resistance against the papaya ring spot virus. Markers linked to these resistance (R) genes were identified using two recombinant inbred line populations, derived from crosses between Cucumis melo Vedrantais and C. melo PI 161375, and between C. melo Vedrantais and C. melo PI 414723, respectively. Using bulked segregant analysis, as well as systematic scoring of the mapping populations, we developed two amplified fragment length polymorphism markers, two random amplified polymorphic DNA markers and five restriction fragment length polymorphism (RFLP) markers linked to this locus. Four of the RFLP sequences bear homology to nucleotide-binding site-leucine-rich repeat R genes, indicating the presence of a significant R-gene cluster in this locus. Our study provides the most closely linked markers published so far for these important traits. It also improves the resolution of the whole linkage group IX, which was difficult to order in our previous studies. Two of the markers were converted to cleaved amplified polymorphic sequence markers to facilitate their application in marker-assisted selection. Testing these two markers in several melon lines revealed different marker haplotypes in the melon germplasm and supported multiple, independent origin of the Fusarium races 0 and 2 resistance trait.

Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana

“…conglutinans) which is linked to fusarium wilt resistance were developed in cabbage by Lv et al (2013). There are studies that reported SCAR markers linked to Fox resistance in melon (Brotman et al 2005), cotton (wang et al 2009), ginger (Swetha and Subramanian 2008), chickpea (Sharma et al 2004) and eggplant (Mutlu et al 2008). SCAR markers associated with resistance to Foc4 were developed by wang et al (2010) for banana, namely ScaU1001 and ScaS0901 which amplified in Foc4-resistant banana genotypes but not in susceptible genotypes tested.…”

Breeding melon for resistance to Fusarium wilt: recent developments

“…The first molecular markers linked to this gene, were reported by Brotman et al (2002Brotman et al ( , 2005 using two recombinant inbred line populations developed at ''Institut National de la Recherche AgronomiqueFrance'' (INRA). Based on BSA, Brotman et al (2005) identified a RAPD marker that generated a 1,235-bp fragment linked to the Fom-1 gene in repulsion phase.…”

“…Based on BSA, Brotman et al (2005) identified a RAPD marker that generated a 1,235-bp fragment linked to the Fom-1 gene in repulsion phase. This RAPD was converted to a CAPS marker designated as 62-CAPS (Table 3).…”

An AFLP marker linked to the Pm-1 gene that confers resistance to Podosphaera xanthii race 1 in Cucumis melo

“…In melon, for example, there have been reports of linkage between molecular markers and resistance genes to Fusarium oxysporum f. sp. melonis (Wechter and Dean, 1998;Karsies et al, 2000;Brotman et al, 2005), zucchini yellow mosaic virus (ZYMV: Danin-Poleg et al, 2002), melon necrotic spot virus (MNSV: Baudracco-Arnas and Pitrat, 1996), cucumber mosaic virus (CMV; Dogimont et al, 2000), and papaya ringspot virus-W (PRSV-W: Brotman et al, 2005;Teixeira and Camargo, 2006). In addition, two candidate resistance genes have been identified by positional cloning, one conferring resistance to race 2 of F. oxysporum f. sp.…”