Molecular markers in embryonic stem cells

Nieden, Nicole I. zur; Ruf, L.J; Kempka, G.; Hildebrand, H.; Ahr, Hans-Juergen

doi:10.1016/s0887-2333(01)00071-6

Cited by 64 publications

(23 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…21,22 Indeed, the cardiac gene profiling and induction of spontaneous chronotropic function in the bone marrow cultures was also comparable to that observed during murine ES cell cardiac differentiation. 16,30 Pallante et al Cardiomyogenesis From Bone Marrow Oct3/4 ؉ Cells e7…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow Oct3/4 ⁺ Cells Differentiate Into Cardiac Myocytes via Age-Dependent Paracrine Mechanisms

Pallante

Duignan

Okin

et al. 2007

Circulation Research

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Bone Marrow Oct3/4 ⁺ Cells Differentiate Into Cardiac Myocytes via Age-Dependent Paracrine Mechanisms

Pallante

Duignan

Okin

et al. 2007

Circulation Research

View full text Add to dashboard Cite

show abstract

“…Accordingly, various types of in vitro tests have incorporated gene expression analyses to enhance their performance (Buesen et al, 2009; Gao et al, 2014; Panzica-Kelly et al, 2013; Pennings et al, 2011; Suzuki et al, 2011; Yu et al, 2015; zur Nieden et al, 2001). In the present study, the impact of BHA and MP was evaluated based on the expression patterns of 16 genes that encode regulators of germ layer formation and axial patterning (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Developmental toxicity assessment of common excipients using a stem cell-based in vitro morphogenesis model

Yuan

Marikawa

2017

Food and Chemical Toxicology

View full text Add to dashboard Cite

Various chemical compounds can inflict developmental toxicity when sufficiently high concentrations are exposed to embryos at the critical stages of development. Excipients, such as coloring agents and preservatives, are pharmacologically inactive ingredients that are included in various medications, foods, and cosmetics. However, concentrations that may adversely affect embryo development are largely unknown for most excipients. Here, the lowest observed adverse effect level (LOAEL) to inflict developmental toxicity was assessed for three coloring agents (allura red, brilliant blue, and tartrazine) and three preservatives (butylated hydroxyanisole, metabisulfite, and methylparaben). Adverse impact of a compound exposure was determined using the stem cell-based in vitro morphogenesis model, in which three-dimensional cell aggregates, or embryoid bodies (EBs), recapitulate embryonic processes of body axis elongation and patterning. LOAEL to impair EB morphogenesis was 200 μM for methylparaben, 400 μM for butylated hydroxyanisole, 600 μM for allura red and brilliant blue, and 1,000 μM for metabisulfite. Gene expression analyses of excipient-treated EBs revealed that butylated hydroxyanisole and methylparaben significantly altered profiles of developmental regulators involved in axial elongation and patterning of the body. The present study may provide a novel in vitro approach to investigate potential developmental toxicity of common excipients with mechanistic insights.

show abstract

“…The cytotoxicity assay was performed as described [7], the cytotoxic effects of EGF on 3T3 and ES cells were determined by CCK8. On day 1, 500 cells per 200 µL medium were seeded in a 96-well plate and cultivated with a series of different concentrations of EGF for 10 days.…”

Section: Cytotoxicity Measurementmentioning

confidence: 99%

Assessment of the Developmental Toxicity of Epidermal Growth Factor using Embryonic Stem Cell Test

Chen¹,

Cao²,

Su³

et al. 2014

Trop. J. Pharm Res

View full text Add to dashboard Cite

Purpose: To determine whether epidermal growth factor (EGF) is involved in reproductive developmental toxicity, using the embryonic stem cell test (EST), as well as ascertain how EGF influences embryonic development. Methods: To predict developmental toxicity on the basis of reducing cell viability and inhibition of differentiation of embryonic stem cells, EST was used to assess changes in different blastodermic genes and expression of proteins including ectodermal-specific genes Pax6, NF-H and glial fibrillary acidic protein (GFAP), mesodermal-specific genes BMP4, GATA4, and MyoD, viz, transthyretin (TTR)

show abstract

Molecular markers in embryonic stem cells

Cited by 64 publications

References 7 publications

Bone Marrow Oct3/4 ⁺ Cells Differentiate Into Cardiac Myocytes via Age-Dependent Paracrine Mechanisms

Bone Marrow Oct3/4 ⁺ Cells Differentiate Into Cardiac Myocytes via Age-Dependent Paracrine Mechanisms

Developmental toxicity assessment of common excipients using a stem cell-based in vitro morphogenesis model

Assessment of the Developmental Toxicity of Epidermal Growth Factor using Embryonic Stem Cell Test

Contact Info

Product

Resources

About

Molecular markers in embryonic stem cells

Cited by 64 publications

References 7 publications

Bone Marrow Oct3/4 + Cells Differentiate Into Cardiac Myocytes via Age-Dependent Paracrine Mechanisms

Bone Marrow Oct3/4 + Cells Differentiate Into Cardiac Myocytes via Age-Dependent Paracrine Mechanisms

Developmental toxicity assessment of common excipients using a stem cell-based in vitro morphogenesis model

Assessment of the Developmental Toxicity of Epidermal Growth Factor using Embryonic Stem Cell Test

Contact Info

Product

Resources

About

Bone Marrow Oct3/4 ⁺ Cells Differentiate Into Cardiac Myocytes via Age-Dependent Paracrine Mechanisms

Bone Marrow Oct3/4 ⁺ Cells Differentiate Into Cardiac Myocytes via Age-Dependent Paracrine Mechanisms